Effects of 2,4-dichlorophenoxyacetic acid on polyamine synthesis in Chinese hamster ovary cells

Rivarola, Viviana; Balegno, Héctor

doi:10.1016/0378-4274(91)90101-b

Cited by 16 publications

(7 citation statements)

References 5 publications

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“…Total serum protein, the majority of serum proteins which are synthesized in the liver, is used as an indicator of liver impairment (Yang and Chen 2003). Rivarola and Balegno (1991) reported that the reduction in plasma protein in animals treated with pesticides could be attributed to changes in protein and free amino acid metabolism and their synthesis in the liver. In addition, the decrease in blood protein may be due to loss of protein by either reduced protein synthesis or increased proteolytic activity or degradation (Shakoori et al 1990).…”

Section: Resultsmentioning

confidence: 99%

A comparative study on the effects of a pesticide (cypermethrin) and two metals (copper, lead) to serum biochemistry of Nile tilapia, Oreochromis niloticus

Fırat

Çoğun

Yüzereroğlu

et al. 2011

Fish Physiol Biochem

238

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The present study was designed to compare the responses in freshwater fish Oreochromis niloticus exposed to a synthetic pyrethroid, cypermethrin (CYP); an essential metal, copper (Cu); and a nonessential metal, lead (Pb). Fish were exposed to 0.05 μg/l CYP, 0.05 mg/l Cu, and 0.05 mg/l Pb for 4 and 21 days, and the alterations in serum enzyme activities, metabolite, and ion levels were determined. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities increased in response to CYP, Cu, and Pb exposures at both exposure periods. While elevations in alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) activities and in cholesterol level were observed in pesticide-exposed fish at 4 and 21 days, they increased in Cu- and Pb-exposed fish at 21 days. Although metal-exposed fish showed increases in cortisol and glucose levels at 4 days followed by a return to control levels at the end of the exposure period, their levels elevated in pesticide-exposed fish at both exposure periods. Total protein levels decreased in Pb- and pesticide-exposed fish at 21 days. Na+ and Cl− levels decreased in pesticide-exposed fish at both exposure periods and in Cu- and Pb-exposed fish at 21 days. The exposures of pesticide and metals caused an elevation in K+ level at the end of the exposure period. The present study showed that observed alterations in all serum biochemical parameters of fish-treated pesticide were higher than those in fish exposed to metals.

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Section: Resultsmentioning

confidence: 99%

A comparative study on the effects of a pesticide (cypermethrin) and two metals (copper, lead) to serum biochemistry of Nile tilapia, Oreochromis niloticus

Fırat

Çoğun

Yüzereroğlu

et al. 2011

Fish Physiol Biochem

238

View full text Add to dashboard Cite

show abstract

“…RPR-V caused significant decreases in total protein contents in the treated rats; similarly, decreased protein levels were reported in monozeb-treated rats (Baligar and Kaliwal, 2001) and cypermethrin-treated sheep and suggested that the decline in serum protein was mainly due to the decrease in albumin rather than globulin fraction (Yousef et al, 1998). Further, it was also reported that the reduction in protein content in animals treated with pesticides could be attributed to changes in protein and free amino acid metabolism and their synthesis in liver (Rivarola and Balegno, 1991). In addition, the decrease in blood protein may be due to loss of protein either by reduced protein synthesis or increased proteolytic activity or degradation (Shakoori et al, 1990).…”

Section: Discussionmentioning

confidence: 95%

Hematological and Clinical Chemistry Changes Induced by Subchronic Dosing of a Novel Phosphorothionate (RPR-V) in Wistar Male and Female Rats

Rahman

Siddiqui

2006

Drug and Chemical Toxicology

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A novel phosphorothionate [2-butenoic acid-3-(diethoxy phosphinothioyl)-ethyl ester; RPR-V] synthesized at Indian Institute of Chemical Technology (Hyderabad, India) was studied using subchronic doses of 0.033 (low), 0.066 (medium), and 0.099 (high) mg kg(- 1) in male and female rats daily for 90 days. Continuous treatment with RPR-V caused significant (p < 0.05) decreases in body-weight gain, feed intake, hemoglobin (Hb), hematocrit (Hct), and total erythrocyte count (TEC), whereas total leukocyte count (TLC), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were increased. Similarly, RPR-V caused significant elevation in serum clinical chemistry parameters calcium, phosphorus, creatinine, and chloride contents, whereas protein and glucose levels were depressed in both male and female treated rats after 45 and 90 days of treatment. These alterations were significant when compared with two-way ANOVA showing that these changes were dose- and time-dependent. The effects of low dose were generally not statistically significant, whereas medium and high doses caused significant effects. The changes in male rats were not significant when compared with female rats showing no sexual dimorphism by this compound. Recovery was observed after 28 days post-treatment (withdrawal study), indicating that the compound entered into the system was eliminated from the body, and the blood parameters were improved. Hematological and clinical chemistry parameters can be detected rapidly and hence can be used for prediction and diagnosis of pesticide toxicity. Alterations in these parameters show toxic stress in the treated animals especially on blood and blood-forming organs.

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“…A reduced level in total protein and albumin after Cyp toxicity was also earlier reported by Yousef et al (2003), who propose that the decrease in total protein was mainly due to a reduction in albumin instead of globulin fraction [ 37 ]. Rivarola and Balengo deduced that the decrease in plasma protein, especially albumin, by pesticide treatment might be attributed to changes in protein metabolism in the liver [ 38 ]. The changing serum levels of albumin thus provide valuable evidence of acuteness, progression, and prognosis in liver disease [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Hepatoprotective Effect of Curcumin Nano-Lipid Carrier against Cypermethrin Toxicity by Countering the Oxidative, Inflammatory, and Apoptotic Changes in Wistar Rats

et al. 2023

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This study investigated the potential hepatoprotective activity of curcumin-incorporated nano-lipid carrier (Cur-NLC) against cypermethrin (Cyp) toxicity in adult Wistar male rats. All animals in groups III, IV, V, and VI were subjected to Cyp (50 mg/kg) toxicity for 15 days. Three different doses of Cur-NLC (1, 2.5, and 5 mg/kg/day) were administered orally for 10 days. The toxic effects were evaluated considering the increases in serum hepatic biomarkers alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein and albumin, and lipid peroxidation (LPO), as well as a decrease in antioxidative activity (reduced glutathione (GSH), superoxide dismutase (SOD), and catalase) and the upregulation of inflammatory cytokines (IL-1β, IL-6, and TNF-α). Immunohistochemistry studies of proteins (NF-κB, Apaf-1, 4-HNE, and Bax) showed enhanced expression, and histopathological examination revealed architectural changes in liver cells, indicating liver toxicity in animals. Toxicity was determined by quantitative and qualitative determinations of DNA fragmentation, which show massive apoptosis with Cyp treatment. The administration of Cur-NLC significantly ameliorates all changes caused by Cyp, such as a decrease in the levels of serum liver markers, an increase in antioxidative parameters, a decrease in expression of inflammatory cytokines (IL-1β, IL-6, TNF-α, and NF-κB), and apoptosis (caspases-3, 9, Apaf-1, 4-HNE, and Bax), according to calorimetric and immunohistochemistry studies. The smear-like pattern of DNA is ameliorated similarly to the control at a high dose of Cur-NLC. Furthermore, all histopathological changes were reduced to a level close to the control. In conclusion, Cur-NLC could be a potent nutraceutical that exhibits a hepatoprotective effect against Cyp-induced hepatotoxicity in rats.

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Effects of 2,4-dichlorophenoxyacetic acid on polyamine synthesis in Chinese hamster ovary cells

Cited by 16 publications

References 5 publications

A comparative study on the effects of a pesticide (cypermethrin) and two metals (copper, lead) to serum biochemistry of Nile tilapia, Oreochromis niloticus

A comparative study on the effects of a pesticide (cypermethrin) and two metals (copper, lead) to serum biochemistry of Nile tilapia, Oreochromis niloticus

Hematological and Clinical Chemistry Changes Induced by Subchronic Dosing of a Novel Phosphorothionate (RPR-V) in Wistar Male and Female Rats

Hepatoprotective Effect of Curcumin Nano-Lipid Carrier against Cypermethrin Toxicity by Countering the Oxidative, Inflammatory, and Apoptotic Changes in Wistar Rats

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