Effects of 24-Epibrassinolide on Antioxidant System in Cucumber Seedling Roots Under Hypoxia Stress

Kang, Yunyan; Guo, Shirong; Li, Juan; JiuJu, Duan

doi:10.1016/s1671-2927(07)60046-7

Cited by 18 publications

(17 citation statements)

References 27 publications

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“…Thus, BRs greatly slowed down the increases of ROS levels and alleviated the chilling-induced oxidative damage. This is in agreement with early finding that increased ROS levels caused by hypoxia stress were inhibited by EBR application (Kang et al 2007).…”

Section: Discussionsupporting

confidence: 94%

“…The 7-day-old stock cultures were transferred into the liquid MS medium containing 0.05 mg/l 24-epibrassinolide (EBR; Sigma, USA) which is one of the most active and stable among many kinds of BRs in field trials (Kang et al 2007) for a period of 3 days. After that, the cultures were incubated in the growth chamber set at 4 or 0°C for 5 days and under continuous shaking at 120 r.p.m.…”

Section: Plant Materials and Chilling Treatmentsmentioning

confidence: 99%

“…BRs can stimulate the antioxidant systems to increase the tolerance of rice to salinity (Núñez et al 2003), cucumber seedlings to hypoxia stress (Kang et al 2007), and chickpea to cadmium ). Nonetheless, scarcely any studies have been conducted on the effects of BRs on the antioxidant defense systems and ROS levels in plant cells under chilling stress.…”

Section: Introductionmentioning

confidence: 99%

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Brassinosteroids alleviate chilling-induced oxidative damage by enhancing antioxidant defense system in suspension cultured cells of Chorispora bungeana

et al. 2009

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Brassinosteroids (BRs), a class of plant steroid hormones, play a significant role in the amelioration of various biotic and abiotic stresses. In order to further explore and elaborate their roles in plants subjected to chilling stress, suspension cultured cells of Chorispora bungeana with or without 24-epibrassinolide (EBR) application were exposed to 4 and 0°C for 5 days. The EBR treated cells exhibited higher viability after exposure to low temperatures compared with the control. Under chilling stress, reactive oxygen species (ROS) levels and lipid peroxidation were increased in the cultured cells, which were significantly inhibited by EBR application. The activities of antioxidative enzymes such as ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) were increased during chilling treatments, and these increases were more significant in the EBR applied suspension cells. The EBR treatment also greatly enhanced contents of ascorbic acid (AsA) and reduced glutathione (GSH) under chilling stress. From these results, it can be concluded that EBR could play the positive roles in the alleviation of oxidative damage caused by ROS overproduction through enhancing antioxidant defense system, resulting in improving the tolerance of C. bungeana suspension cultures to chilling stress.

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Section: Discussionsupporting

confidence: 94%

Section: Plant Materials and Chilling Treatmentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Brassinosteroids alleviate chilling-induced oxidative damage by enhancing antioxidant defense system in suspension cultured cells of Chorispora bungeana

et al. 2009

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“…The length of plant exposure to BRs, the frequency of application, the application mode and the type and dose of BRs can also substantially affect the growth/yield promoting activity of these compounds (Fariduddin et al 2003(Fariduddin et al , 2008Khripach et al 2003;Núñez et al 2003;Ramraj et al 1997;Sasse 2003;Vlašánková et al 2009). The degree of BRs influence on plants depends also on plant species and genotype (Ali et al 2008b;Hnilička et al 2007; Kang et al 2007;Khripach et al 2003;Núñez et al 2003;Ramraj et al 1997;Sairam 1994b;Shahbaz et al 2008;Torres-Ruiz et al 2007;Vardhini and Rao 2003;Vlašánková et al 2009;Zhao and Chen 2003).…”

Section: Introductionmentioning

confidence: 99%

The effect of brassinosteroids on the morphology, development and yield of field-grown maize

et al. 2010

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The response of two field-grown inbred lines of maize (Zea mays L.) and their F1 hybrid to the application of 10 -8 -10 -14 M solutions of 24-epibrassinolide or synthetic androstane analogue of castasterone in V3/4 and V6/ 7 developmental stages was followed during the vegetative and early reproductive phases of plant development. Brassinosteroids (BRs) significantly affected (either positively or negatively, depending on the genotype and the developmental stage they were applied) the height of plants during the early weeks after their application, but not the final plant height nor the number of leaves. Spraying of plants with BRs in V3/4 developmental stage usually also increased the length of the 7th to 10th leaf, whereas the application in V6/7 developmental stage had the opposite effect. The beginning of the reproductive phase of plant development and the course of flowering was strongly influenced by the application of BRs. Treatment of plants in V3/4 stage delayed and treatment of plants in V6/7 stage advanced the dates of anthesis and silking, regardless of the type of BR used, its concentration or plant genotype. The influence of BRs on the development of the secondary ear was the least pronounced in the F1 hybrid; in both inbred lines it strongly depended on the concentrations of BRs used. Various yield parameters were also affected by treatment of plants with BRs, but this effect depended on the developmental stage during which the application of BRs occured, the plant genotype, the type of BR and its concentration.

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“…Thus, it seems that BRs can be a mediator of particular aspects of the developmental responses to hypoxia in plants. Our recent study showed that root applied EBR, a highly active and stable steroidal hormone, alleviated oxidative damage induced by root-zone hypoxia in cucumber seedlings and rendered the plants more tolerant to it (Khripach et al 2000;Kang et al 2007). However, there is little information available about the effect of BRs on anaerobic carbohydrate catabolism, a fundamental component of the adaptation of plant tissue to hypoxia in any plant species.…”

Section: Introductionmentioning

confidence: 99%

Effect of root applied 24-epibrassinolide on carbohydrate status and fermentative enzyme activities in cucumber (Cucumis sativus L.) seedlings under hypoxia

Kang

Guo

et al. 2008

Plant Growth Regul

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The effects of 24-epibrassinolide (EBR) added to nutrient solution on growth of cucumber (Cucumis sativus L.) under root-zone hypoxia were investigated. Cucumber seedlings were hydroponically grown for 8 days in normoxic and hypoxic nutrient solutions with and without addition of EBR at 1 lg l -1 . EBR exerted little influence on plant performance in the normoxic nutrient solution, while the chemical alleviated root-zone hypoxia-induced inhibition of root and shoot growth and net photosynthetic rate (Pn). EBR added to hypoxic nutrient solution caused an increase in the concentration of fructose, sucrose, and total soluble sugars in the roots but not in the leaves. Root-zone hypoxia enhanced the activities of lactate dehydrogenase (LDH), alcohol dehydrogenase (ADH), and pyruvate decarboxylase in the roots. Interestingly, EBR further enhanced ADH activity but lowered LDH activity in hypoxic roots. These results suggest that EBR added to hypoxic nutrient solution may stimulate the photosynthate allocation down to roots and the shift from lactate fermentation to alcohol fermentation in hypoxic roots, resulting in the increase in ATP production through glycolysis and the avoidance of cytosolic acidosis and eventually enhanced tolerance of cucumber plants to root-zone hypoxia.

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Effects of 24-Epibrassinolide on Antioxidant System in Cucumber Seedling Roots Under Hypoxia Stress

Cited by 18 publications

References 27 publications

Brassinosteroids alleviate chilling-induced oxidative damage by enhancing antioxidant defense system in suspension cultured cells of Chorispora bungeana

Brassinosteroids alleviate chilling-induced oxidative damage by enhancing antioxidant defense system in suspension cultured cells of Chorispora bungeana

The effect of brassinosteroids on the morphology, development and yield of field-grown maize

Effect of root applied 24-epibrassinolide on carbohydrate status and fermentative enzyme activities in cucumber (Cucumis sativus L.) seedlings under hypoxia

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