Effects of 4-MBC and triclosan in embryos of the frog Pelophylax perezi

Martins, Diana; Monteiro, Marta S.; Soares, Amadeu M.V.M.; Quintaneiro, Carla

doi:10.1016/j.chemosphere.2017.03.038

Cited by 45 publications

(21 citation statements)

References 68 publications

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“…It looks like that TCS exposed hatchlings needed more energy for overcoming the stress of TCS even during the post exposure period as LDH activity declined over 14 days values but was still more than the respective 7 days values. Increase in the activity of LDH with the concentration of TCS in the present study gets support from Martins et al 61 and Jha and Mitra-Mazumder 64 . For production of energy, conversion of pyruvate to lactate is catalyzed by LDH in the anaerobic pathway and the activity of LDH has been widely used as an indicator of cellular injury and changes in energy production in stressed animals 23,65,66 .…”

Section: Discussionsupporting

confidence: 88%

“…GSTs help in generating less toxic and more hydrophilic products of a xenobiotic. Olivera et al 60 and Martins et al 61 also observed higher GST activity in the TCS exposed embryos of zebrafish and Pelophylax perezi , respectively. Observation of Martinez-Paz 62 that TCS increased expression of GSTd3, GSTe1, GSTo1 and GSTt1 corroborates the concentration dependent increase in GST activity of the hatchlings till 7 days in our study.…”

Section: Discussionmentioning

confidence: 89%

See 1 more Smart Citation

Biomarkers for the toxicity of sublethal concentrations of triclosan to the early life stages of carps

Dar

Sharma

Singh

et al. 2020

Sci Rep

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Accumulation, contents of protein, non-enzymatic antioxidant glutathione (GSH and GSSG), lipid peroxidation product (melondialdehyde-MDA) and organic acids (fumarate, succinate, malate and citrate), and activities of neurological (acetylcholinesterase-AChE), detoxification (glutathione S-transferase-GST) and metabolic (lactate dehydrogenase-LDH, aspartate transaminase-AST and alanine transaminase-ALT) enzymes were recorded in the hatchlings of Cyprinus carpio, Ctenopharyngodon idella, Labeo rohita and Cirrhinus mrigala after 7 and 14 days exposure and 10 days post exposure (recovery period) to sublethal concentrations (0.005, 0.01, 0.02 and 0.05 mg/L) of triclosan, a highly toxic and persistent biocide used in personal care products. Accumulation was maximum between 7–14 days at 0.01 mg/L for C. carpio and L. rohita but at 0.005 mg/L for C. idella and C. mrigala. No triclosan was observed at 0.005 mg/L in C. carpio and C. mrigala after recovery. Significant decline in protein, glutathione and acetylcholinesterase but increase in glutathione S-transferase, lactate dehydrogenase, aspartate transaminase, alanine transaminase, melondialdehyde and organic acids over control during exposure continued till the end of recovery period. Integrated biomarker response (IBR) analysis depicted higher star plot area for glutathione and glutathione S-transferase during initial 7 days of exposure, thereafter, during 7–14 days of exposure and the recovery period, higher star plot area was observed for acetylcholinesterase, aspartate transaminase, alanine transaminase and organic acids. Higher star plot area was observed for protein in all the species throughout the study. The study shows that L. rohita is most sensitive and glutathione, acetylcholinesterase, aspartate transaminase and alanine transaminase are the biomarkers for the toxicity of sublethal concentrations of TCS.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 89%

Biomarkers for the toxicity of sublethal concentrations of triclosan to the early life stages of carps

Dar

Sharma

Singh

et al. 2020

Sci Rep

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“…Likewise, TCS stimulated the GST activity in mussel digestive gland indicating that TCS may be a substrate for phase II enzymes (Canesi et al, 2007). Such results are concordant with the previous reports of the effects of TCS on other species such as zebrafish (Oliveira et al, 2009); swordtail fish (Liang et al, 2013); Daphnia magna (Peng et al, 2013); Yellow Catfish (Ku et al, 2014); copepod (Park et al, 2017); frog (Martins et al, 2017); and Brachionus koreanus ( (Han et al, 2016). In contrast to the above, no significant effect on GST activity was noticed at lower concentrations (Falisse et al, 2017;Oliveira et al, 2009).…”

Section: Discussionsupporting

confidence: 90%

Assessment of triclosan impact on enzymatic biomarkers in an Indian major carp, Catla catla

Hemalatha

Rangasamy

Nataraj

et al. 2019

JoBAZ

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Background: The present study was designed to determine the median lethal concentration (96-h LC50) of triclosan (TCS) and to investigate the effect of a sublethal concentrations of TCS on the activities of glutamic oxaloacetic transaminases (GOT), glutamic pyruvic transaminases (GPT), and glutathione S-transferase (GST) in the gill, liver, and muscle of an Indian major carp Catla catla. Results: In this study, static renewal procedure was followed to determine the 96 LC50 value. The results indicated that the 96-h LC50 of TCS for C. catla was 0.36 mg L −1. 1/10th, 1/25th, and 1/50th of 96-h LC50 value (0.036, 0.014, 0.007 mg L −1) were selected for sublethal studies, and the fish were examined every 5 days for 20 days. The results indicated that TCS exposure to fish at three different sublethal concentrations significantly (p < 0.05, p < 0.01) enhanced GOT, GPT, and GST enzyme activity in all the tissues with increased dose and exposure period. Conclusion: Prolonged exposure (20 days) to TCS at sublethal concentrations induces severe physiological alterations in Catla catla, and these biomarkers can be used as a mechanistic tool for evaluating the toxicity-derived alterations in fish.

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“…The oxidative potential of TCS was also evident in the rotifer Brachionus koreanus , detected as ROS overproduction and enhanced GST activity, in addition to transcriptional modulation of cytochromes, antioxidant genes Gst , Gpx , Sod , and Cat and chaperons (Table 3) [120]. Moreover, TCS inhibited Sod and phospholipid hydroperoxide glutathione peroxidase ( Phgpx ) expression in the liver of Bufo gargarizans tadpoles [121] and induced GST in Pelophylax perezi frog larvae [122].…”

Section: Oxidative Stressmentioning

confidence: 99%

Triclosan: An Update on Biochemical and Molecular Mechanisms

Alfhili

Lee

2019

Oxidative Medicine and Cellular Longevity

102

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Triclosan (TCS) is a synthetic, chlorinated phenolic antimicrobial agent commonly used in commercial and healthcare products. Items made with TCS include soaps, deodorants, shampoos, cosmetics, textiles, plastics, surgical sutures, and prosthetics. A wealth of information obtained from in vitro and in vivo studies has demonstrated the therapeutic effects of TCS, particularly against inflammatory skin conditions. Nevertheless, extensive investigations on the molecular aspects of TCS action have identified numerous adversaries associated with the disinfectant including oxidative injury and influence of physiological lifespan and longevity. This review presents a summary of the biochemical alterations pertaining to TCS exposure, with special emphasis on the diverse molecular pathways responsive to TCS that have been elucidated during the present decade.

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Effects of 4-MBC and triclosan in embryos of the frog Pelophylax perezi

Cited by 45 publications

References 68 publications

Biomarkers for the toxicity of sublethal concentrations of triclosan to the early life stages of carps

Biomarkers for the toxicity of sublethal concentrations of triclosan to the early life stages of carps

Assessment of triclosan impact on enzymatic biomarkers in an Indian major carp, Catla catla

Triclosan: An Update on Biochemical and Molecular Mechanisms

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