Effects of 6-Benzylaminopurine and Indole-3-acetic Acid on Growth and Root Development of Banana Explants in Micropropagation

Khan, Arifa; Bashir, Arsalan; Erum, Shazia; Khatak, Jabar Zaman Khan; Muhammad, Aish

doi:10.17582/journal.sja/2021/37.1.9.13

Cited by 5 publications

(4 citation statements)

References 10 publications

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“…In accordance with the statement of [16] that the function of the hormone cytokinin can encourage shoots and inhibit root growth, while auxin does the opposite, encouraging root growth and inhibiting shoot growth. This is also supported by the statement of [17] that high concentrations of BAP can inhibit adventitious meristem elongation and transformation into complete plants. The optimal concentration on the number of shoots was obtained in treatment 4 (MS+1.5) which was 1.11.…”

Section: Resultsmentioning

confidence: 53%

“…The optimal concentration on the number of shoots was obtained in treatment 4 (MS+1.5) which was 1.11. This is probably due to the endogenous hormone levels of auxin in the explants being less than the cytokinin given at a level of 1.5, so it can increase the growth rate of shoots, but if the concentration is more than 1.5 ppm it can reduce multiplication and reduce shoot elongation and growth [17]. This can be proven by the number of shoots obtained in treatment 6, the number of shoots is at least 0.11.…”

Section: Resultsmentioning

confidence: 99%

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In vitro seed germination of local red pummelo (Citrus maxima (Burm.) Merr.) from Pangkep Regency, South Sulawesi

Tuwo

Tambaru

Izzah

2022

IOP Conf. Ser.: Earth Environ. Sci.

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South Sulawesi is one of the centers of citrus development in Indonesia. Potential production centers are in Pangkep Regency. One of the cultivars that are cultivated and become a commercial farm is the red pummelo. Red pummelo has higher total phenolic and carotenoid content, so it is a good source of antioxidants and has the potential to become a superior functional food. The aim of this study was to obtain the optimal concentration of benzyl amino purine (BAP) hormone on in vitro germination of red pummelo. Planting material used in this study was seeds extracted from fruit obtained from citrus plantations in Pangkep Regency, South Sulawesi. After sterilization, the seeds were planted on MS media with the treatment of BAP 0; 0.5; 1; 1.5; 2; and 2.5 ppm. Observations were made for 8 weeks with the parameters of the number of shoots, number of leaves and number of roots. The data were analyzed using the Kruskal Wallis test at the 5% level, and if there was a significant effect, it was continued with the Mann-Whitney test. The results showed that the BAP had a significant effect on the parameters of the number of roots with a value of 0.11 and the number of shoots with a value of 0.16. The results of the Mann-Whitney further test showed that there was a significant difference between the 2.5 ppm and 0 ppm BAP treatments on the number of roots and the 2.5 ppm and 1.5 ppm BAP treatments on the number of shots.

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Section: Resultsmentioning

confidence: 53%

Section: Resultsmentioning

confidence: 99%

In vitro seed germination of local red pummelo (Citrus maxima (Burm.) Merr.) from Pangkep Regency, South Sulawesi

Tuwo

Tambaru

Izzah

2022

IOP Conf. Ser.: Earth Environ. Sci.

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“…Further, MS media supplemented with NAA (2.65 μM) + 0.2% activated charcoal successfully induced rooting in bananas ( Shashikumar et al., 2017 ). Besides, IAA (2.8 μM) in combination with BAP (22.19 μM) has also been widely used by different groups for in vitro rooting of banana shoots ( Khan et al., 2021 ; Quiñonez et al., 2021 ).…”

Section: Micropropagation Of Bananamentioning

confidence: 99%

Biotechnological interventions in banana: current knowledge and future prospects

Justine

Kaur

Savita

et al. 2022

Heliyon

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“…Medium formulations also play an essential role in the growth and accumulation of bioactive compounds in a plant. Using different auxin hormones, such as IAA, IBA, and NAA can cause differences in the rate of root formation (Sevik and Guney, 2013;Khan et al, 2021). In this experiment, formulations "A" and "B" were the same liquid medium but different hormones.…”

Section: Effects Of Various Plant Growth Regulators On Panax Ginseng ...mentioning

confidence: 99%

Mass Production of Panax ginseng C.A. Mey. Root Cultures in Indonesia

Chandra¹,

Vianney²,

Christie³

et al. 2021

SJA

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P. ginseng can be found in several countries, such as Korea (common name: Insam), China (common name: Rénshēn), and Japan (common name Ninjin). It has gained importance in traditional medicine because it can restore fatigue (Mahady et al., 2000). Panax means "all-healing", which comes from the belief that ginseng can cure all diseases in humans. Its benefits include modulating various ion channels Abstract | Panax ginseng C.A. Mey. is one of the most well-known plants in traditional medicine that contains bioactive compounds called ginsenosides. It is widely used as raw material in many pharmaceutical industries in Indonesia. However, to supply for this purpose, they still rely on imports. PT. Kalbe Farma (through its subsidiary, PT. Bintang Toedjoe), University of Surabaya (Ubaya), and Hanbang-Bio Laboratory (holding company of Kyung Hee University) established the Kalbe Ubaya Hanbang-Bio Laboratory (KUH Lab), a collaboration initiated to achieve the independence of raw materials. This laboratory is devoted to developing the plant tissue culture protocol for the mass production of P. ginseng root cultures. This paper is the first report of in vitro P. ginseng culture in Indonesia. The initial stage of the mass production focused on optimizing the culture conditions: inoculum weight (100 g, 150 g, 200 g), medium volume (12 L, 13 L, 14 L), medium formulation ("A", "B"), and incubation temperature (15 °C to 20 °C and 21°C to 25 °C). Based on the biomass yield and ginsenosides content, it was concluded that the optimum growth condition was 150 g of the initial inoculum grown in 13 L of media using formulation B and incubation at 21°C to 25 °C. In the long-term, KUH Lab aims to produce P. ginseng on an industrial scale to sufficiently supply the demands for P. ginseng in the country. Furthermore, this laboratory also intends to make standardized Indonesian herbal materials by using plant tissue culture.

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Effects of 6-Benzylaminopurine and Indole-3-acetic Acid on Growth and Root Development of Banana Explants in Micropropagation

Cited by 5 publications

References 10 publications

In vitro seed germination of local red pummelo (Citrus maxima (Burm.) Merr.) from Pangkep Regency, South Sulawesi

In vitro seed germination of local red pummelo (Citrus maxima (Burm.) Merr.) from Pangkep Regency, South Sulawesi

Biotechnological interventions in banana: current knowledge and future prospects

Mass Production of Panax ginseng C.A. Mey. Root Cultures in Indonesia

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