Effects of a Bacterial Lipid Byproduct on Human Pulp Fibroblasts In Vitro

Ho, Yung‐Chuan; Chang, Yu‐Chao

doi:10.1016/j.joen.2006.12.022

Cited by 11 publications

(8 citation statements)

References 26 publications

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“…H33258 fluorescence assay using a multiwell fluorescence scanner was developed for screening cytotoxicity to cells cultured in 24‐well microtiter plates by Skehan (1995). H33258 fluorescence was measured according to previous studies (Chang & Chou 2001, Ho & Chang 2007). The cells were plated at an initial density of 2 × 10 4 cells per well into 24‐well culture plates and allowed to attach for 48 h. Culture medium was replaced with fresh DMEM, and then cells were exposed to various concentrations of CHX for 2 h. Finally, the medium was removed and the plates frozen at −80 °C and stored frozen until ready for further processing.…”

Section: Methodsmentioning

confidence: 99%

Cytotoxicity of chlorhexidine on human osteoblastic cells is related to intracellular glutathione levels

Lee

et al. 2010

Int Endodontic J

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Section: Methodsmentioning

confidence: 99%

Cytotoxicity of chlorhexidine on human osteoblastic cells is related to intracellular glutathione levels

Lee

et al. 2010

Int Endodontic J

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“…Human dental pulp fibroblasts were cultured from molars as previously described, with minor modification (Adachi et al, 2007;Ho and Chang, 2007). Molars without any cavity or trauma were obtained from three healthy donors aged 15 to 30 yrs after they provided informed consent.…”

Section: Human Dental Pulp Fibroblast Culturementioning

confidence: 99%

Synergy of TLR2 and H1R on Cox-2 Activation in Pulpal Cells

et al. 2009

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Although pulp fibroblasts are a major cell type in dental pulp, their roles in microbial recognition and pulpal inflammation are not well-understood. Considering the pivotal role of Toll-like receptors (TLRs) in the recognition of micro-organisms, we hypothesized that TLRs on pulp fibroblasts may induce inflammatory signals in dental pulp. In human pulp fibroblasts, TLR2, 3, 4, and 5 were constitutively expressed. Stimulation of TLR2 and 3 induced the expression of pro-inflammatory genes such as CXCL10, CCL5, and/or Cox-2 in pulp fibroblasts. Interestingly, histamine synergistically activated TLR2-mediated Cox-2 expression and PGE(2) production. The synergistic effect of histamine is mediated by histamine receptor-1 (H1R). Studies on the intra-cellular signaling pathways revealed that p38 activation is required for the synergistic activation of Cox-2 by TLR2 and histamine. Analysis of these data suggests that TLR2 on pulp fibroblasts, in concert with H1R, can induce an inflammatory response during microbial infection in dental pulp.

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“…Cytotoxicity was measured by the Hoechst 33258 (H33258) fluorescence assay as described previously (17,18). H33258 is an ultraviolet-excited blue bisbenzimidazole dye that selectively intercalates into A-T rich regions of DNA, undergoing a fluorescence enhancement in the process.…”

Section: Cytotoxicity Assaymentioning

confidence: 99%