Effects of a hypoproteic diet on myosin-V immunostained myenteric neurons and the proximal colon wall of aging rats

Schoffen, João Paulo Ferreira; Soares, Andréia Assunção; Freitas, Priscila de; Buttow, Nilza Cristina; Natali, Maria Raquel Marçal

doi:10.1016/j.autneu.2005.08.005

Cited by 28 publications

(36 citation statements)

References 35 publications

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“…The mucosa thickness decreased in G3 animals compared to G1 animals (p<0.05), which has also been observed in other studies (Viteri and Schneider 1974, Rodrigues et al 1985, Da CostaRibeiro et al 1987, Torrejais et al 1995, Natali et al 2000, Schoffen et al 2005, De Azevedo et al 2007, Hermes et al 2008. Conversely, the mucosa was less reduced in G4 (7.4%) than in G3 (13.5%) compared to well-nourished G1 animals (p<0.05), as illustrated in Figure 2.…”

Section: Probiotics Protect the Jejunum Of Malnourished Ratsupporting

confidence: 83%

“…The morphological alterations induced by malnutrition have been thoroughly investigated. Studies using experimental proteinmalnutrition models have shown a decreased thickness of bowel wall layers, such as reductions of the mucosa (Rodrigues et al 1985, Firmansyah et al 1989, Torrejais et al 1995, Natali et al 2000, Brandão et al 2003, Gurmini et al 2005, Schoffen et al 2005, De Azevedo et al 2007, Hermes et al 2008, enterocyte height (Brandão et al 2003, De Azevedo et al 2007, Hermes et al 2008, villi height (Firmansyah et al 1989, Natali et al 2000, Gurmini et al 2005, crypt depth (Firmansyah et al 1989, Hermes et al 2008, and muscular layer thickness (Torrejais et al 1995, Natali et al 2000, Brandão et al 2003, De Azevedo et al 2007). Therefore, strategies to correct or reduce the damage caused by malnutrition must be investigated.…”

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confidence: 99%

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Probiotics protect the intestinal wall of morphological changes caused by malnutrition

Azevedo

Hermes-Uliana

Lima

et al. 2014

An. Acad. Bras. Ciênc.

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This study sought to morphometrically analyze the jejunal wall of protein-malnourished rats administered a probiotic supplement. The sample consisted of recently weaned Wistar rats (Rattus norvegicus) distributed among four groups: animals given a commercial diet (G1, n = 4); animals given the same ration as G1 plus a probiotic supplement (G2, n = 4); animals given a 4% protein diet (G3, n = 4); and animals given the same ration as G3 plus a probiotic supplement (G4, n = 4). After 12 weeks, part of the jejunum was harvested and subjected to routine histological processing. Transverse sections with a thickness of 3 µm were stained with HE, and histochemical techniques were used to assay for glycoconjugates, including staining with periodic acid-Schiff (PAS) + diastase, Alcian Blue (AB) solution at pH 2.5, and Alcian Blue solution at pH 1.0. Morphometric analysis of the bowel wall showed that the probiotic culture used in this study induced hypertrophy of several layers of the jejunal wall in well-nourished animals and reduced the bowel wall atrophy usually observed in protein-malnourished animals. Neither malnutrition nor the use of probiotics altered the relationship between the number of goblet cells and the number of enterocytes.

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Section: Probiotics Protect the Jejunum Of Malnourished Ratsupporting

confidence: 83%

mentioning

confidence: 99%

Probiotics protect the intestinal wall of morphological changes caused by malnutrition

Azevedo

Hermes-Uliana

Lima

et al. 2014

An. Acad. Bras. Ciênc.

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“…Similar results were found by Freitas et al (12) , who observed a significant 37.9% reduction in neuronal density in the jejunum in diabetic animals compared with non-diabetic animals. Other studies performed by our group have analyzed different segments of the gastrointestinal tract, including the ileum (3,22,46) , proximal colon (15,24,25,34,39) , and stomach (14) and showed similar results. This reduction in the number of myenteric neurons results from hyperglycemia, which initiates several biochemical events, such as disturbances in the polyol pathway, formation of AGEs, activation of the protein kinase C and hexosamine pathways, and glycolysis.…”

Section: Discussionsupporting

confidence: 64%

Supplementation with 0.1% and 2% vitamin e in diabetic rats: analysis of myenteric neurons immunostained for myosin-V and nNOS in the jejunum

Tronchini

Trevizan

Tashima

et al. 2012

Arq. Gastroenterol.

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-Context -Diabetes mellitus is a disease characterized by hyperglycemia that, when allowed to progress long-term untreated, develops vascular and neurological complications, which are responsible for the development of alterations in the enteric nervous system in diabetic patients. In the gastrointestinal tract, diabetes mellitus promotes motor and sensory changes, and in the reflex function of this system, causing gastroparesis, diarrhea, constipation, megacolon, slow gastrointestinal transit, gastric stasis and dilation with decreased or increased peristaltic contractions. Several studies have shown that oxidative stress is the main responsible for the vascular and neurological complications affecting the enteric nervous system of diabetics. Objective -The effects of 0.1% and 2% vitamin E on myosin-V-and nNOS-immunoreactive neurons in the jejunum of diabetic rats were investigated. Methods -Thirty rats were divided into the groups: normoglycemic, normoglycemic treated with 0.1% vitamin E, normoglycemic treated with 2% vitamin E, diabetic, diabetic treated with 0.1% vitamin E, and diabetic treated with 2% vitamin E. The neuronal density and areas of neuron cell bodies were determined. Results -Diabetes (diabetic group) significantly reduced the number of myosin-V-immunoreactive neurons compared with the normoglycemic group. The diabetic treated with 0.1% vitamin E and diabetic treated with 2% vitamin E groups did not exhibit a greater density than the D group (P>0.05). Nitrergic density did not change with diabetes (P>0.05). The areas of myosin-V-and nNOS-immunoreactive neurons significantly increased in the normoglycemic treated with 2% vitamin E and diabetic groups compared with the normoglycemic group. Conclusion -Supplementation with 2% vitamin E had a neurotrophic effect only in the area of myosin-V-immunoreactive neurons compared with the diabetic group. HEADINGS -Vitamin E. Diabetes mellitus, experimental. Jejunum. Neurons. Myosin type V. Nitric oxide sinthase. Rats.

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“…) Contrariwise, another author who verified a neuroprotective effect for cerebellum granule cells affirms that DHA reduction reactions do not produce ROS, as DHA rapidly reverts to ascorbate, which can be used by the cell or released into the extracellular fluid, features antioxidant properties, and does not generate free radicals (Wilson 2002 Our results reveal there was an increase in neuronal cellular profile with age and that the ganglionic arrangement was maintained, although with visible spaces between the cellular bodies of different sizes in the ganglia of 348 and 428-day-old animals. The maintenance of the ganglionic arrangement and the variety in areas of neuronal cell bodies were also observed in the myenteric ganglia of rats subjected to protein malnutrition using the myosin-V immunohistochemical technique (Schoffen et al 2005), which evidences the rearrangement ability of these neurons. Several authors describe the great adaptive rearrangement of the plexuses, evidencing enteric neural plasticity (Santer and Baker 1988, Saffrey and Burnstock 1994, Hanani et al 2003.…”

Section: Effect Of Ascorbic Acid On Myenteric Neurons Of Ratsmentioning

confidence: 83%

“…The myosin-V immunohistochemical technique used in our study is highly restricted to the neuronal cytoplasm, cellular bodies and nerve fibers, without marking glial cells, and is indicated by several authors as a specific marker for neurons (Drengk et al 2000, Buttow et al 2003, Zanoni et al 2003, Schoffen et al 2005, Marese et al 2007). …”

Section: Effect Of Ascorbic Acid On Myenteric Neurons Of Ratsmentioning

confidence: 95%

Administering ascorbic acid to rats undergoing ageing processes: effects on myosin-V immunoreactive myenteric neurons

Mello

Marese

Brancalhão

et al. 2013

An. Acad. Bras. Ciênc.

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During the ageing process the enteric nervous system undergoes morphofunctional changes, such as enteric neurodegeneration. Neuronal death can be attributed to increase radicals free, and ascorbic acid (AA), known antioxidant, could minimize damage cause by oxidative stress. The objective of this study is to analyse the behaviour of morphoquantative myenteric neurons in the duodenum of adult Wistar rats with aged 90 (C90), 345 (E345) and 428 (E428) days, as well as animals of the same age who received ascorbic acid supplementation for 120 days (EA345 and EA428). Whole-mount preparations of muscle layer from the duodenum of the animals were immunostained by the method myosin V. 80 microscopic fields were quantified (14.8 mm2/animal) and measured 100 neuronal cell bodies per animal. During the aging process, there was a reduction in neuronal density in all animals groups, indicating that the effects of age were not attenuated with AA supplementation. The increase in the neuronal area of the cell bodies in 428-day-old animals proved the influence of age on this parameter. There was no observed a neuroprotective effect of AA (1 mL/g body weight) on the neuronal population myenteric myosin V immunoreactive.

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Effects of a hypoproteic diet on myosin-V immunostained myenteric neurons and the proximal colon wall of aging rats

Cited by 28 publications

References 35 publications

Probiotics protect the intestinal wall of morphological changes caused by malnutrition

Probiotics protect the intestinal wall of morphological changes caused by malnutrition

Supplementation with 0.1% and 2% vitamin e in diabetic rats: analysis of myenteric neurons immunostained for myosin-V and nNOS in the jejunum

Administering ascorbic acid to rats undergoing ageing processes: effects on myosin-V immunoreactive myenteric neurons

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