2016
DOI: 10.1155/2016/2876916
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Effects of a Nonthermal Atmospheric Pressure Plasma Jet on Human Gingival Fibroblasts for Biomedical Application

Abstract: Nonthermal atmospheric pressure plasma jets (APPJ) have been developed and applied in biomedical research as a cancer treatment or bacterial sterilization. However, the drawback of APPJ on normal oral cells during plasma treatment and underlying cell death mechanisms have not been studied and clearly explained, although there is known to be an influence from reactive oxygen species (ROS). Hence, this study investigates whether and how a nonthermal atmospheric pressure air plasma jet kills human normal gingival… Show more

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Cited by 11 publications
(7 citation statements)
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“…Three minutes of plasma application was more cytotoxic than 1 minute. This data is in accordance to some recent studies that demonstrated dose‐dependent cellular effects in response to cold plasma treatment . Furthermore, LTP treatment is able to get selective killing effect between normal gingival cells and cancer cells due to sensitivity against reactive species …”
Section: Discussionsupporting
confidence: 92%
See 1 more Smart Citation
“…Three minutes of plasma application was more cytotoxic than 1 minute. This data is in accordance to some recent studies that demonstrated dose‐dependent cellular effects in response to cold plasma treatment . Furthermore, LTP treatment is able to get selective killing effect between normal gingival cells and cancer cells due to sensitivity against reactive species …”
Section: Discussionsupporting
confidence: 92%
“…This data is in accordance to some recent studies that demonstrated dose-dependent cellular effects in response to cold plasma treatment. 33,34 Furthermore, LTP treatment is able to get selective killing effect between normal gingival cells and cancer cells due to sensitivity against reactive species. 30,35 The histologic and immunohistochemical results also showed safety biologic response for LTP treatment.…”
Section: Discussionmentioning
confidence: 99%
“…For plasma treatment at a dimmer voltage above 140 V, shattered cell membranes and fragments of broken membranes were observed. This might be associated with cell surface blebbing and breakage of actin filaments (Lee & Kim, 2016). Also, a previous study suggested that the ROS could impose oxidative stress on the cells and dissociating cell membrane proteins (Lin et al, 2017).…”
Section: Resultsmentioning
confidence: 97%
“…The temperature of the plasma plume released was about 35 to 50°C for the first 20 s of operation (Lin et al, 2017). However, the treatment time is also an important factor in regulating the dosage of ROS released (Lee & Kim, 2016). The results indicated that the NTAPPJ system based on dimmer control produced tunable charged species and photon energy, that induced a different level of effects to the biophysical characteristics of cells, ranging from reduced cell adhesion to apoptosis and eventually cell membrane destructions.…”
Section: Resultsmentioning
confidence: 99%
“…Unlike osteoblasts, the proliferation of gingival fibroblasts treated with He-APPJ for 5 minutes was not recovered. Some studies have referred to this continuous cell detachment after plasma radiation exposure as “delayed cell detachment” [ 28 29 ]. The continuous loss of cells is not due to reversible cell detachment, but to the apoptotic behavior of the cells after plasma treatment.…”
Section: Discussionmentioning
confidence: 99%