Effects of a Preparation Containing Pantogam, Succinic Acid, and Chitosan on Activities of the Glutathione System and NADPH-Generating Enzymes in Rat Tissues under Conditions of Cerebral Ischemia/Reperfusion

Сафонова, О. А.; Попова, Т. Н.; Сливкин, А. И.; Talmi, Yu.

doi:10.1007/s10517-015-2927-4

Cited by 6 publications

(5 citation statements)

References 2 publications

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“…Известно, что соли цинка при хроническом действии на организм оказывают токсический эффект на все органы и ткани, в особенности на центральную нервную систему. Так, по данным литературы, увеличение концентрации соединений цинка может приводить к развитию нейродегенеративных заболеваний центральной нервной системы [1,2]. Добыча, обработка и производство цинка способствуют его интенсивному рассеянию в биоценозах.…”

Section: Fad -флавинадениндинуклеотидunclassified

Evaluation of the zinc succinate toxic eﬀect on the cerebral cortex of rat

Piavchenko

Alekseev

Seryogina

et al. 2019

jour

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Обоснование. Антропогенная деятельность человека способствует включению соединений цинка в биологический круговорот на тех территориях, где есть загрязнение данным металлом. Это приводит к накоплению данного вещества в органах и тканях, что может оказывать нейротоксическое воздействие на организм. Цель. Оценить токсический эффект сукцината цинка на структуру и функцию коры больших полушарий головного мозга лабораторных крыс. Материалы и методы. Исследование проводили на 12 крысах-самцах линии Wistar возрастом 2 мес. Животных разделяли на 2 группы-контрольную и экспериментальную, по 6 крыс в каждой. Животным экспериментальной группы в течение 30 дней внутрижелудочно вводили раствор, содержащий сукцинат цинка в дозе 100 мг/кг, крысы из группы 1 служили интактным контролем. Проведен флуоресцентный анализ активности коферментов мозга, для которого использовано возбуждение при длинах волн 365 и 450 нм, что соответствует восстановленной форме никотинамидадениндинуклеотида (NADH) и флавинадениндинуклеотида (FAD); а также морфологический анализ моторной коры больших полушарий головного мозга. Результаты. Анализ спектров флуоресценции продемонстрировал статистически значимое снижение ее интенсивности в экспериментальной группе для кофермента NADH (0,855 [0,092]) по сравнению с контролем (0,709 [0,062]); р<0,05. Отличий по интенсивности спектров флуоресценции для кофермента FAD не установлено. Морфологический анализ выявил наличие токсических и дистрофических процессов в коре головного мозга у животных экспериментальной группы: полнокровие сосудов, местами-расширение периваскулярного пространства, небольшое количество нейронов в виде клеток-теней. Заключение. Действие сукцината цинка на структуры коры головного мозга характеризуется снижением интенсивности флуоресценции кофермента NADH и отсутствием изменений флуоресценции кофермента FAD, что может свидетельствовать об инициации митохондриальной дисфункции и нарушении процессов оксигенации нейронов. Морфологический анализ демонстрирует развитие дистрофических изменений. Ключевые слова: сукцинат цинка, моторная кора, спектральный анализ флуоресценции, никотинамидадениндинуклеотид восстановленный, флавинадениндинуклеотид.

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Section: Fad -флавинадениндинуклеотидunclassified

Evaluation of the zinc succinate toxic eﬀect on the cerebral cortex of rat

Piavchenko

Alekseev

Seryogina

et al. 2019

jour

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“…Treatment with a complex preparation containing pantogs, succinic acid and chitosan normalizes the concentration of reduced glutathione and the activity of glutathione peroxidase, glutathione reductase in animals with experimental hypoxia/reperfusion of the brain. These results are explained by the suppression of free radical oxidation and the normalization of the antioxidant system associated with the neuroprotective, antihypoxic and antioxidant properties of these substances, their involvement in the regulation of cellular metabolism in pathological conditions accompanied by oxidative stress (Safonova et al, 2015). Derivatives of succinic acid showed promising antifungal and antibacterial activity against various test microorganisms and possess antioxidant activity (Raghavendra et al, 2017).…”

Section: Introductionmentioning

confidence: 98%

Assessment of antioxidant properties of grain concentrate and oxidant-antioxidant status pigs after its inclusion in ration feeding

Kuznetsova

Klimova

Uchasov

et al. 2018

Potr. S. J. F. Sci.

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A grain concentrate was developed for use in bread baking based on whole-ground fermented wheat grain, to enhance that the beneficial properties have fermented wholegrain buckwheat grains in an amount of 20% by weight of the fermented wheat. For the fermentation of grain used dry complex enzyme preparation comprising cellulose, β-glucanase and xylanase (producing Penicillin canescens), dissolved in a buffer based on succinic acid. Under the action of the drug, the micro structure surface of grain was changed. It is established that the character of the change in surface micro structure of wheat and buckwheat grain is the same. The results of the study of the content of vitamin E, flavonoids and antioxidant activity in wheat grains, buckwheat and grain concentrate are obtained by different technologies. The results show that grain concentrates from wheat grain with the addition of 20% buckwheat grains prepared using a solution of enzyme preparation of cellulolytic action in a buffer, based on succinic acid has a high antioxidant activity. As a biological model for studying changes oxidant-antioxidant status of the organism under stress when included in a diet designed grain concentrate, used pigs, that are under stress, caused by weaning them from sows and transportation. Investigated the following parameters oxidant-antioxidant status of the organism pigs: the level of malondialdehyde, ceruloplasmin, vitamins A, E and C in the blood of animals. It is concluded that, to improve the oxidative status of the piglets after weaning period recommended addition of concentrate fodder ration of grain wheat and buckwheat prepared using a solution of an enzyme preparation buffered cellulolytic action on the basis of succinic acid. The developed grain concentrate can be used for making the manufacture of cereal products, including grain bread included in the diet of people who live in conditions of oxidative stress.

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“…It is well established that the chronic exposure of mammalian organisms to zinc salts induces toxic effects in nearly all organs and tissues, and especially in the central nervous system [1,2]. According to the literature, the increase of the zinc and succinic acid concentration may lead to the development of neurodegenerative diseases of the central nervous system [3,4]. It has been shown that the increase of zinc concentration in the body might cause dysfunctions of the immune system and provoke a deficiency in the content of other chemical compounds such as iron and copper.…”

Section: Introductionmentioning

confidence: 99%

A complex morphofunctional approach for zinc toxicity evaluation in rats

Piavchenko

Alekseev

Stelmashchuk

et al. 2020

Heliyon

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Anthropogenic activity causes the introduction of zinc compounds into the biological cycle in mining and processing sites and its accumulation in organs and tissues, causing systemic toxicity. A cumulative effect of zinc is predominantly neurotoxic and it also affects the respiratory, cardiovascular and digestive systems. This study evaluates the effects of single-dose intragastric administration of 100 mg/kg zinc succinate on the structure and function of organs and tissues in male Wistar rats 1 month after treatment. The presented morphofunctional approach for the toxicity evaluation included the study of behavioral responses using the automated Laboras® complex, fluorescent spectral analysis of the NADH and FAD activity and histological evaluation of animal organs and tissues. The results of the behavioral activity assessment showed a significant decrease in animals' motor activity, whereas the fluorescence spectra analysis demonstrated a decrease in coenzyme NADH without the reduction of FAD levels. We detected toxic and dystrophic changes in the cerebral cortex, heart, lungs and liver tissues. Our original multiparametric approach enables a comprehensive assessment of the long-term toxic effects of the metal salts such as zinc succinate, especially in the cerebral cortex at the doses much lower than the acute LD 50 reported for the common zinc salts.

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Effects of a Preparation Containing Pantogam, Succinic Acid, and Chitosan on Activities of the Glutathione System and NADPH-Generating Enzymes in Rat Tissues under Conditions of Cerebral Ischemia/Reperfusion

Cited by 6 publications

References 2 publications

Evaluation of the zinc succinate toxic eﬀect on the cerebral cortex of rat

Evaluation of the zinc succinate toxic eﬀect on the cerebral cortex of rat

Assessment of antioxidant properties of grain concentrate and oxidant-antioxidant status pigs after its inclusion in ration feeding

A complex morphofunctional approach for zinc toxicity evaluation in rats

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