Effects of Acetan on Production of Bacterial Cellulose by <i>Acetobacter xylinum</i>

Ishida, Takehiko; Sugano, Yasushi; Nakai, Tomoya; Shoda, Makoto

doi:10.1271/bbb.66.1677

Cited by 66 publications

(34 citation statements)

References 14 publications

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“…Functionalization or modification of bacterial cellulose has mainly been achieved by chemical or mechanical modifications of the cellulose matrix or via changing culturing conditions (7,22), whereas only a few attempts at genetic engineering have been made (13,23). However, genetic engineering may allow a greater range of materials to be produced, by enabling fine control over cellulose synthesis genes and production of proteincellulose composite biomaterials.…”

Section: Significancementioning

confidence: 99%

Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain

Florea

Hagemann

Santosa

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

194

216

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Bacterial cellulose is a strong and ultrapure form of cellulose produced naturally by several species of the Acetobacteraceae. Its high strength, purity, and biocompatibility make it of great interest to materials science; however, precise control of its biosynthesis has remained a challenge for biotechnology. Here we isolate a strain of Komagataeibacter rhaeticus (K. rhaeticus iGEM) that can produce cellulose at high yields, grow in low-nitrogen conditions, and is highly resistant to toxic chemicals. We achieved external control over its bacterial cellulose production through development of a modular genetic toolkit that enables rational reprogramming of the cell. To further its use as an organism for biotechnology, we sequenced its genome and demonstrate genetic circuits that enable functionalization and patterning of heterologous gene expression within the cellulose matrix. This work lays the foundations for using genetic engineering to produce cellulose-based materials, with numerous applications in basic science, materials engineering, and biotechnology.

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Section: Significancementioning

confidence: 99%

Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain

Florea

Hagemann

Santosa

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

194

216

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“…The production of cellulose and water-soluble polysaccharides was measured as described previously (9,23). To purify the cellulose, a precipitate of culture broth obtained by centrifugation was washed with deionized water and treated with 0.1 M NaOH at 80°C for 20 min.…”

mentioning

confidence: 99%

Control of Acetic Acid Fermentation by Quorum Sensing via N -Acylhomoserine Lactones in Gluconacetobacter intermedius

2008

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A number of gram-negative bacteria regulate gene expression in a cell density-dependent manner by quorum sensing via N-acylhomoserine lactones (AHLs). Gluconacetobacter intermedius NCI1051, a gram-negative acetic acid bacterium, produces three different AHLs, N-decanoyl-L-homoserine lactone, N-dodecanoyl-L-homoserine lactone, and an N-dodecanoyl-L-homoserine lactone with a single unsaturated bond in its acyl chain, as determined by liquid chromatography-tandem mass spectrometry. Two genes encoding an AHL synthase and a cognate regulator were cloned from strain NCI1051 and designated ginI and ginR, respectively. Disruption of ginI or ginR abolished AHL production, indicating that NCI1051 contains a single set of quorum-sensing genes. Transcriptional analysis showed that ginI is activated by GinR, which is consistent with the finding that there is an inverted repeat whose nucleotide sequence is similar to the sequence bound by members of the LuxR family at position ؊45 with respect to the transcriptional start site of ginI. A single gene, designated ginA, located just downstream of ginI is transcribed by read-through from the GinR-inducible ginI promoter. A ginA mutant, as well as the ginI and ginR mutants, grew more rapidly in medium containing 2% (vol/vol) ethanol and accumulated acetic acid at a higher rate with a greater final yield than parental strain NCI1051. In addition, these mutants produced larger amounts of gluconic acid than the parental strain. These data demonstrate that the GinI/GinR quorum-sensing system in G. intermedius controls the expression of ginA, which in turn represses oxidative fermentation, including acetic acid and gluconic acid fermentation.Diverse bacteria communicate intercellularly to regulate the transcription of specific target genes in a cell density-dependent manner. This cell-cell communication is termed quorum sensing. Bacteria monitor their cell density by measuring the concentration of self-produced diffusible signal molecules termed autoinducers or pheromones. Quorum sensing is used to regulate diverse physiological functions, including secondary metabolite production, swimming and swarming motility, conjugal plasmid transfer, biofilm formation, and virulence (25, 27).Many gram-negative bacteria use N-acylhomoserine lactones (AHLs) as quorum sensors to regulate gene expression in concert with cell density (7,8). Two important proteins are involved in AHL-dependent quorum sensing: a LuxR-type protein, which is an AHL-dependent transcriptional regulator, and a LuxI-type protein, which synthesizes AHLs. In general, LuxR-type proteins bind their cognate AHLs once the concentration of the AHL reaches a critical level, and the resulting complex activates the transcription of specific target genes.Acetic acid bacteria are gram-negative, obligately aerobic bacteria with the ability to oxidize ethanol and sugars into their corresponding organic acids. Acetobacter and Gluconacetobacter are used to produce vinegar because of their ability to oxidize ethanol into acetic acid and their...

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“…Water soluble polysaccharide was prepared from the culture medium as previously reported by Ishida et al . 2) In brief, the culture medium was centrifuged to remove cellulose and cells, and the supernatant was treated with two volumes of ethanol. The precipitate was air dried and dissolved in 0.1 M NaOH, and then an equal volume of chloroform was added to this solution.…”

mentioning

confidence: 99%

Structural Analysis of the Water-soluble Carbohydrate from Asaia bogorensis by NMR Spectroscopy

Kato¹,

Mizuno²,

Nakai³

et al. 2007

J. Appl. Glycosci.

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The water-soluble extracellular polysaccharide produced by Asaia bogorensis, an acetic acid bacterium, was investigated. It was suggested that the water-soluble polysaccharide seemed to be a fructan as only fructose was detected as a constituent sugar when it was hydrolyzed by trifluoroacetic acid. To confirm the glycosidic linkage of the fructan, 1 H NMR spectroscopy and 13 C NMR spectroscopy were carried out. From these results, Asaia fructan was identified as levan, which is a β-2,6 linked fructan, similar to the polysaccharide produced by Gluconacetobacter xylinus. This β-2,6-linked levan-type fructan was produced only when sucrose was used as a carbon source.

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Effects of Acetan on Production of Bacterial Cellulose by Acetobacter xylinum

Cited by 66 publications

References 14 publications

Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain

Engineering control of bacterial cellulose production using a genetic toolkit and a new cellulose-producing strain

Control of Acetic Acid Fermentation by Quorum Sensing via N -Acylhomoserine Lactones in Gluconacetobacter intermedius

Structural Analysis of the Water-soluble Carbohydrate from Asaia bogorensis by NMR Spectroscopy

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