2004
DOI: 10.1016/j.ab.2003.09.026
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Effects of acetic acid treatment on plant chromosome structures analyzed by atomic force microscopy

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Cited by 24 publications
(17 citation statements)
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“…The chromatin-fiber level structures in the present study are consistent with those of previous studies using different species and preparations (Tamayo 2003a, 2004. This suggests that no artifacts at this scale are caused by the difference in the preparation protocols, since the 30 nm chromatin fiber, a universal component of the eukaryotic chromosome, has been observed as having almost the same structures in all these reports included in this study.…”
Section: Discussionsupporting
confidence: 93%
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“…The chromatin-fiber level structures in the present study are consistent with those of previous studies using different species and preparations (Tamayo 2003a, 2004. This suggests that no artifacts at this scale are caused by the difference in the preparation protocols, since the 30 nm chromatin fiber, a universal component of the eukaryotic chromosome, has been observed as having almost the same structures in all these reports included in this study.…”
Section: Discussionsupporting
confidence: 93%
“…In our preparation, we observed granules and fibers 50-70 nm in diameter. Similarly sized structures were observed in previous AFM studies of mitotic metaphase chromosome of human (Tamayo 2003a), barley (Sugiyama et al . , 2004, and mouse sperm chromatin .…”
Section: Discussionsupporting
confidence: 60%
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“…AFM analysis revealed that the optimal concentration was 30%; at lower levels sufficiently clean chromosomes samples could not obtain and the chromosome surface structures could not be observed, and at higher levels the chromosome structures were significantly damaged, although the cellular contaminants were effectively removed. 7) Similar studies identified chromosomal damages induced by treatments with NaCl 8) and heavy ion radiation. 9) EB, a heterocyclic organic compound, can insert between base pairs, 10) resulting in increasing DNA rigidity and lengthening of DNA.…”
mentioning
confidence: 62%
“…Nathewet et al (2009) indicated that 60% acetic acid was effective for cleaning the cytoplasm of the somatic cells in octoploid strawberry plants when the chromosome samples were stained with DAPI. However, Sugiyama et al (2004) demonstrated that the chromosomes of barley (Hordeum vulgare) were damaged by a 45% acetic acid treatment at 37 C overnight. Therefore, it could be considered that high concentrations of acetic acid are harmful to chromosomes.…”
Section: Effect Of Acetic Acid Concentration (Experiments 2)mentioning
confidence: 99%