Effects of Achillea millefolium Aqueous Extract on Electrophysiological Properties of Rat Barrel Cortex Neurons

Salari, Elham; Shamsizadeh, Ali; Noorbakhsh, S. Mohammad; Ayoobi, Fatemeh; Sheibani, Vahid; Oryan, Sharbanoo

doi:10.17795/jjnpp-37758

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…As it is known, the influence of various factors on the activity of neurons is usually researched on laboratory animals [ 23 , 24 ]; therefore, Wistar rats were used in our work. During the experiment, the animals were kept in standard conditions, with free access to water and food, in TECNIPLAST type IV S plastic cages, with 3–4 rats in one cage (in accordance with the rules of animal accommodation).…”

Section: Methodsmentioning

confidence: 99%

Electrophysiological Activity and Survival Rate of Rats Nervous Tissue Cells Depends on D/H Isotopic Composition of Medium

et al. 2021

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The deuterium content modification in an organism has a neuroprotective effect during the hypoxia model, affecting anxiety, memory and stress resistance. The aim of this work was to elucidate the possible mechanisms of the medium D/H composition modification on nerve cells. We studied the effect of an incubation medium with a 50 ppm deuterium content compared to a medium with 150 ppm on: (1) the activity of Wistar rats’ hippocampus CA1 field neurons, (2) the level of cultured cerebellar neuron death during glucose deprivation and temperature stress, (3) mitochondrial membrane potential (MMP) and the generation of reactive oxygen species in cultures of cerebellar neurons. The results of the analysis showed that the incubation of hippocampal sections in a medium with a 50 ppm deuterium reduced the amplitude of the pop-spike. The restoration of neuron activity was observed when sections were returned to the incubation medium with a 150 ppm deuterium content. An environment with a 50 ppm deuterium did not significantly affect the level of reactive oxygen species in neuron cultures, while MMP decreased by 16–20%. In experiments with glucose deprivation and temperature stress, the medium with 50 ppm increased the death of neurons. Thus, a short exposure of nerve cells in the medium with 50 ppm deuterium acts as an additional stressful factor, which is possibly associated with the violation of the cell energy balance. The decrease in the mitochondrial membrane potential, which is known to be associated with ATP synthesis, indicates that this effect may be associated with the cell energy imbalance. The decrease in the activity of the CA1 field hippocampal neurons may reflect reversible adaptive changes in the operation of fast-reacting ion channels.

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Section: Methodsmentioning

confidence: 99%

Electrophysiological Activity and Survival Rate of Rats Nervous Tissue Cells Depends on D/H Isotopic Composition of Medium

et al. 2021

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“…One of the neighboring whiskers (in same row with PW) with the least neural responses was considered as AW. The rest of rat whiskers were shortened by a scissor to avoid miss-stimulations (Salaria et al, 2016).…”

Section: Extracellular Single-unit Recordingmentioning

confidence: 99%

Modulatory Effects of Memantine on Neuronal Response Properties in Rat Barrel Cortex

Nasiri

Fatemi

Azin

et al. 2021

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ntroduction: Memantine as N-Methyl-D-aspartic acid (NMDA) receptor antagonist is used in some neurological disorders. It has been reported that memantine has modulatory effects on the somatosensory information processing in healthy subjects. This study investigated the effect of memantine on electrophysiological properties of barrel cortex neurons in male rats. Methods: Single unit recording was used to evaluate the electrophysiological properties of barrel cortex neurons. The neural responses to the principal whisker (PW), adjacent whisker (AW) and combined displacement of two whiskers [20 ms inter-stimulus intervals (ISIs)] were recorded before and 2 hours after memantine gavage (10 mg/kg). A condition test ratio (CTR) was calculated for assessing inhibitory interactions. Results: Two hours after memantine gavage, neuronal ON and OFF responses to PW deflection were decreased. Also, CTR for both ON and OFF responses was decreased following memantine administration. Conclusions: The current study demonstrated that memantine modified neural response properties in the rat barrel cortex.

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Achillea millefolium is beneficial as an add-on therapy in patients with multiple sclerosis: A randomized placebo-controlled clinical trial

et al. 2019

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Effects of Achillea millefolium Aqueous Extract on Electrophysiological Properties of Rat Barrel Cortex Neurons

Cited by 3 publications

References 24 publications

Electrophysiological Activity and Survival Rate of Rats Nervous Tissue Cells Depends on D/H Isotopic Composition of Medium

Electrophysiological Activity and Survival Rate of Rats Nervous Tissue Cells Depends on D/H Isotopic Composition of Medium

Modulatory Effects of Memantine on Neuronal Response Properties in Rat Barrel Cortex

Achillea millefolium is beneficial as an add-on therapy in patients with multiple sclerosis: A randomized placebo-controlled clinical trial

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