Effects of acute and long-term diazepam administrations on neutrophil activity: a flow cytometric study

Silva, Fábio Ribeiro da; Lazzarini, Ricardo; Sá-Rocha, Luiz Carlos de; Morgulis, M. S. F. A.; Massoco, Cristina de Oliveira; Palermo-Neto, João

doi:10.1016/j.ejphar.2003.08.046

Cited by 24 publications

(23 citation statements)

References 39 publications

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“…5). Caffeine increases the cAMP level via two routes; it antagonizes adenosine receptors [17,22] and inhibits intracellular phosphodiesterase (PDE) [7,8]. As to the former aspect, we found that the inhibitory effect of adenosine on I GABA in rat DRG neurons is mediated by subunit A 1 adenosine receptors and not by A 2A receptors [23].…”

Section: Discussionmentioning

confidence: 82%

Caffeine Suppresses GABA Receptor-Mediated Current in Rat Primary Sensory Neurons via Inhibition of Intracellular Phosphodiesterase

Yang

Ren

et al. 2015

Neurophysiology

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In acutely isolated rat primary sensory neurons, the effects of caffeine on GABA receptormediated current (I GABA ) were investigated using a whole-cell patch clamp technique. We found that applications of GABA (10-1000 µM) induced inward currents in a concentration-dependent manner; the currents manifested obvious desensitization. Pretreatment with caffeine (0.01-100 µM) suppressed I GABA in a noncompetitive manner; caffeine shifted the concentrationresponse curve for GABA downwards compared to the control. Theophylline showed a similar and stronger inhibitory effect on I GABA . Isolated application of 1 µM diazepam enhanced I GABA , while pretreatment with 10 µM caffeine and 1 µM diazepam suppressed this current. Intracellular application of the protein kinase A inhibitor H-8 dramatically weakened the inhibitory effect of caffeine on I GABA . Because primary afferent depolarization is related to GABA A receptors, our results suggest that caffeine might antagonize presynaptic inhibitory effects of primary afferents, probably via inhibition of intracellular phosphodiesterase.

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Section: Discussionmentioning

confidence: 82%

Caffeine Suppresses GABA Receptor-Mediated Current in Rat Primary Sensory Neurons via Inhibition of Intracellular Phosphodiesterase

Yang

Ren

et al. 2015

Neurophysiology

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“…Мида золам ингибирует ЛПС индуцируемую активацию циклоокси геназы 2 и индуцибельной NO синтазы в макрофагах, а так же продукцию свободных радикалов [71]. Длительное примене ние бензодиазепинов приводит к стойкому иммуносупрессив ному эффекту [72,73].…”

Section: седация и иммуномодуляцияunclassified

Psychomotor Agitation in Critically Ill Patients and the Role of Sedation

ФедарауМ.¹,

Комполо²,

Papadokos³

2012

rmt

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О Б Щ А Я Р Е А Н И М А Т О Л О Г И Я , 2 0 1 2 , V I I I ; 6 62 www.niiorramn.ru Существующая проблема психомоторных расст ройств, таких как возбуждение, тревога и делирий, многие годы привлекает внимание врачей и сестер отделений реани матологии, а также руководства больниц. Эти состояния мо гут напрямую влиять на оказание медицинской помощи больным, увеличивать их метаболические потребности и в конечном счете увеличивать заболеваемость и летальность. Данные состояния также связаны с увеличением продолжи тельности и стоимости нахождения больных в отделениях реаниматологии. В настоящее время дыхательная недоста точность является наиболее частой причиной поступления больных в отделение реаниматологии и сопровождается вы сокой летальностью. Однако только 16% больных, которым проводится искусственная вентиляция легких, умирают не посредственно от дыхательной недостаточности [1][2][3]. Сре ди внелегочных факторов, влияющих на летальность боль ных в критическом состоянии, возбуждение и делирий оста ются недооцененными и плохо изученными. В связи с этим необходима разработка и применение рекомендаций с сис темным и организационным подходом, направленных на профилактику и лечение данных нарушений.В течение последнего десятилетия были приняты реко мендации и протоколы, посвященные проведению седации в отделении реаниматологии и направленные на поддержание оптимального уровня комфорта и безопасности больных в критическом состоянии [4,5]. Седация и обезболивание рас сматриваются как жизненно важные мероприятия в течение всего периода госпитализации. Это приводит к увеличению усилий медицинского персонала, направленных на седацию и анальгезию у этой категории больных. В последнее время кли ницисты столкнулись со значительной нехваткой седативных препаратов, что повлияло на локальные протоколы отделений реаниматологии по всему миру. Наше растущее понимание важности изменений ментального статуса у больных в крити ческом состоянии и их влиянии на исход в свою очередь по влияло на тактику седации в отделениях реаниматологии. Это привело к изменению оценки и методов лечения больных.Critically ill patients often receive analgesia and sedation to keep them comfortable and to prevent agitation and delirium, which are serious, common and yet frequently underdiagnosed forms of brain dysfunction in intensive care environment with unclear but often unfavorable contribution to patient outcomes. Apart from delirium assessment and screening the most important aspect of ICU management is understanding the drugs given to patients and their specific advantages and disadvantages. It is crucial for clinicians and units to develop guidelines and protocols for the administration of sedatives depending on the type of patient population. Newer drugs, such as dexmedetomidine and remifentanyl, should be intro duced and extensively studied. The immunomodulating effects of sedation also must be explored, as these properties may greatly affect outcome. With an increased understanding of sedative drugs will come an improved ability to use multiple dru...

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“…During stress, the central nervous system is able to influence the immune system response through mediation over neurovegetative functions since both systems share receptors for cytokines, neurotransmitters, hormones and neuropeptides (Blalock, 1984;Silva et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Passionflower supplementation in diets of Japanese qualis at rearing and laying periods

et al. 2010

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-The objective of this study was to assess the effect of the inclusion of passionflower (Passiflora alata) in diets on performance, behavior, heterophil/lymphocyte ratio (H/L), corticosterone plasma levels (CPL) and immune response of quails. The study was carried out using 192 birds, distributed in randomized blocks with 4 diets (0, 125, 250 and 375 and 0, 250, 500 and 750 mg of passionflower/kg of feed for the rearing and laying period, respectively), 8 replicates and 6 birds per experimental unit. The rearing period began when birds were 28 days of age and it lasted for 15 days. In this phase, it was evaluated daily feed intake, daily weight gain, feed conversion, viability, tonic immobility time, intensity of injuries, behavior, heterophil/lymphocyte ratio, and corticosterone plasma levels. Laying phase started when birds were 43 days of age and it finished when they were 140 days of age; it was compesed of six 14-day cycles in which it evaluated feed intake, feed conversion, egg production and quality, viability, tonic immobility time, intensity of injuries, behavior, heterophil/ lymphocyte ratio, corticosterone plasma levels and immune response. In the rearing period, the use of passionflower in the diet of quails influenced the behavioral parameters, and the birds became calmer, mainly at the dosage of 375 mg/kg of ration.However, in the laying period, passionflower was efficient in reducing stress in the quails, and the most promising results are observed when higher dosages of the phytotherapic were applied. Nevertheless, the dose of 750 mg/kg of feeeding reduces egg production, though it does not influence quality of eggs neither immune response of the quails.Key Words: behavior, corticosterone, heterophil/lymphocyte ratio, immune response, stress, tonic immobility Efeitos da utilização de passiflora em rações de codornas japonesas nas fases de recria e postura

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Effects of acute and long-term diazepam administrations on neutrophil activity: a flow cytometric study

Cited by 24 publications

References 39 publications

Caffeine Suppresses GABA Receptor-Mediated Current in Rat Primary Sensory Neurons via Inhibition of Intracellular Phosphodiesterase

Caffeine Suppresses GABA Receptor-Mediated Current in Rat Primary Sensory Neurons via Inhibition of Intracellular Phosphodiesterase

Psychomotor Agitation in Critically Ill Patients and the Role of Sedation

Passionflower supplementation in diets of Japanese qualis at rearing and laying periods

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