Effects of acute ethanol and/or diazepam exposure on immediate and delayed hippocampal metabolite levels in rats anesthetized with isoflurane

Carton, Louise; Auger, F.; Laloux, Charlotte; Durieux, Nicolas; Kyheng, Maéva; Potey, Camille; Bergeron, Sandrine; Rolland, Benjamin; Deguil, Julie; Bordet, Régis

doi:10.1111/fcp.12764

Cited by 3 publications

(1 citation statement)

References 54 publications

(59 reference statements)

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“…6D6T for GABA receptor was used and its cocrystallized ligand in diazepam binding site was used to provide coordinates to the software. After successful completion of docking protocol, binding energies of the compounds were compared with diazepam (Carton et al, 2022). The results showed that binding energies of gallic acid (-20 kcal/mol), kaempferol (-16 kcal/mol) were much more negative than the diazepam (-9 kcal/mol), indicating that gallic and kaempferol have much higher binding a nities than the diazepam.…”

Section: In Silico Studymentioning

confidence: 99%

Anticonvulsant potential of Grewia tiliaefolia in pentylenetetrazole induced epilepsy: insights from in vivo and in silico studies

et al. 2023

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Epilepsy is a chronic disease that affects millions of people worldwide and also a major cause of morbidity and mortality. Available antiepileptic drugs have series of side effects which encouraged us to explore different medicinal plants used against neurological disorders in traditional Indian medicinal system (TIMS). Therefore, we explored the antiepileptic potential of the Grewia tiliaefolia (Tiliaeceae) known for its neuroprotective properties in TIMS. Initially, aerial parts of G. tiliaefolia were subjected to extraction with increasing order of polarity viz. hexane, chloroform and methanol. Antioxidant potential of hexane, chloroform and methanol extracts of G. tiliaefolia was evaluated by DPPH, total antioxidant assay, reducing power assay and DNA nicking assay. Additionally, quantitative antioxidant assays were also conducted to quantify total phenolic and total avonoid content. The methanol extract was found to possess very high antioxidant activity; therefore, its anticonvulsant potential was explored in PTZ induced epilepsy in mice. The methanol extract had shown signi cant anticonvulsant activity at 400 mg/kg. It had signi cantly increased the latency to occurrence of myoclonic jerks, generalized tonic clonic seizures (GTCS) and signi cantly decreased the duration of the GTCS and seizure severity score. The Grewia tiliaefolia methanol extract was further screened by HPLC for detection of polyphenolic compounds, among which gallic acid and kaempferol were present in higher amount and were further analyzed by in silico study to predict their possible binding sites and type of interactions these compounds show with gamma amino butyric acid (GABA) receptor and glutamate α amino-3-hydroxyl-5-methyl-4isoxazolepropionic acid (AMPA) receptor. It was revealed that gallic acid and kaempferol had shown agonistic interaction with GABA receptor and antagonistic interaction with Glutamate AMPA receptor. We concluded that G. tiliaefolia showed potent anticonvulsant potential due to the signi cant antioxidant activity possibly because of gallic acid and kaempferol. safe up to 2000 mg/kg, 1/10th (200 mg/kg) of the safe dose was selected. To obtain the dose-response relationship three doses are required due to which one lower dose (1/20th, 100 mg/kg) and one higher dose (1/5th, 400 mg/kg) were selected.

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Section: In Silico Studymentioning

confidence: 99%

Anticonvulsant potential of Grewia tiliaefolia in pentylenetetrazole induced epilepsy: insights from in vivo and in silico studies

et al. 2023

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Promising strategies for the prevention of alcohol-related brain damage through optimised management of acute alcohol withdrawal: A focussed literature review

Quelch,

Lingford-Hughes,

John

et al. 2024

J Psychopharmacol

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There is an increasing awareness of the link between chronic alcohol consumption and the development of cognitive, behavioural and functional deficits. Currently, preventative strategies are limited and require engagement in dedicated long-term rehabilitation and sobriety services, the availability of which is low. The acute alcohol withdrawal syndrome is an episode of neurochemical imbalance leading to autonomic dysregulation, increased seizure risk and cognitive disorientation. In addition to harm from symptoms of alcohol withdrawal (e.g. seizures), the underpinning neurochemical changes may also lead to cytotoxicity through various cellular mechanisms, which long-term, may translate to some of the cognitive impairments observed in Alcohol-Related Brain Damage (ARBD). Here we review some of the pharmacological and neurochemical mechanisms underpinning alcohol withdrawal. We discuss the cellular and pharmacological basis of various potential neuroprotective strategies that warrant further exploration in clinical populations with a view to preventing the development of ARBD. Such strategies, when integrated into the clinical management of acute alcohol withdrawal, may impact large populations of individuals, who currently face limited dedicated service delivery and healthcare resource.

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Revealing Anticonvulsant Potential of Grewia tiliaefolia in Pentylenetetrazole induced Epilepsy in mice: In vitro, in vivo and, in silico analysis

Rajput

Sharma

Kumar

et al. 2022

Preprint

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Epilepsy is a chronic disease that affects millions of people worldwide and also a major cause of morbidity and mortality. Available antiepileptic drugs have series of side effects which encouraged us to explore different medicinal plants used against neurological disorders in traditional Indian medicinal system (TIMS). Therefore, we explored the antiepileptic potential of the Grewia tiliaefolia (Tiliaeceae) known for its neuroprotective properties in TIMS. Initially, aerial parts of G. tiliaefolia were subjected to extraction with increasing order of polarity viz. hexane, chloroform and methanol. Antioxidant potential of hexane, chloroform and methanol extracts of G. tiliaefolia was evaluated by DPPH, total antioxidant assay, reducing power assay and DNA nicking assay. Additionally, quantitative antioxidant assays were also conducted to quantify total phenolic and total flavonoid content. The methanol extract was found to possess very high antioxidant activity; therefore, its anticonvulsant potential was explored in PTZ induced epilepsy in mice. The methanol extract had shown significant anticonvulsant activity at 400 mg/kg. It had significantly increased the latency to occurrence of myoclonic jerks, generalized tonic clonic seizures (GTCS) and significantly decreased the duration of the GTCS and seizure severity score. The Grewia tiliaefolia methanol extract was further screened by HPLC for detection of polyphenolic compounds, among which gallic acid and kaempferol were present in higher amount and were further analyzed by in silico study to predict their possible binding sites and type of interactions these compounds show with gamma amino butyric acid (GABA) receptor and glutamate α amino-3- hydroxyl-5-methyl-4-isoxazolepropionic acid (AMPA) receptor. It was revealed that gallic acid and kaempferol had shown agonistic interaction with GABA receptor and antagonistic interaction with Glutamate AMPA receptor. We concluded that G. tiliaefolia showed potent anticonvulsant potential due to the significant antioxidant activity possibly because of gallic acid and kaempferol.

show abstract

Effects of acute ethanol and/or diazepam exposure on immediate and delayed hippocampal metabolite levels in rats anesthetized with isoflurane

Cited by 3 publications

References 54 publications

Anticonvulsant potential of Grewia tiliaefolia in pentylenetetrazole induced epilepsy: insights from in vivo and in silico studies

Anticonvulsant potential of Grewia tiliaefolia in pentylenetetrazole induced epilepsy: insights from in vivo and in silico studies

Promising strategies for the prevention of alcohol-related brain damage through optimised management of acute alcohol withdrawal: A focussed literature review

Revealing Anticonvulsant Potential of Grewia tiliaefolia in Pentylenetetrazole induced Epilepsy in mice: In vitro, in vivo and, in silico analysis

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