Effects of Acute Exposure to Metribuzin on Some Hematological, Biochemical and Histopathological Parameters of Common Carp (Cyprinus carpio L.)

Abstract

The aim of the study was to evaluate acute toxic effects of the preparation Sencor 70 WG (metribuzin 70% W/V) on hematological, biochemical indices and histology of the common carp (Cyprinus carpio L.). In carp exposed for 96 h to Sencor 70 WG in the concentration of 250.2 mg/L, showed significantly lower (p<0.01) values of plasma total proteins, albumins, total globulins, triacylglycerols, lactate dehydrogenase, lactate, inorganic phosphate, hematocrit, hemoglobin concentration, mean erythrocyte volume, the leucocrite value, lymphocyte, and significantly higher (p<0.01) values of glucose, ammonia, calcium, monocytes, neutrophile granulocytes, developmental forms myeloid sequence and basophiles compared to the control group. Histopathological examination revealed hyaline degeneration of the epithelial cells of renal tubules of the caudal kidney. This alteration of kidney resulted in hypoproteinemia, followed by generation of transudate in body cavity.

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“…Velisek and colleagues (2011) also reported an increase in glucose levels in plasma of common carp after long-term exposure to terbutryn, as a response to metabolic stress. Similar effects were observed in common carp (Velisek et al, 2009) and goldfish after acute poisoning with the herbicide Sencor. However, chronic exposure to prometryn and simazine did not influence plasma glucose levels in common carp (Velisek et al, 2012;2013).…”

“…In the previous study, we found increased relative counts of stab neutrophils and monocytes in fish exposed to Sencor . Similarly, other authors reported increased counts of monocytes, and stab and segmented neutrophils in common carp after chronic exposure to atrazine, as well as acute poisoning with metribuzin (Svobodova & Pecena, 1988;Velisek, Svobodova, Piackova, & Sudova, 2009). Odo et al (2017) reported increased monocytes count in African catfish exposed to insecticide Cyperdicot.…”

“…The decreased plasma LDH activity could indicate a decrease in the glycolytic process resulted from herbicide toxicity. Similarly, acute exposure of common carp to the triazine herbicide metribuzin decreased LDH activity in plasma (Velisek et al, 2009). Previously, Velisek et al (2013) reported no changes of plasma LDH activity in common carp after treatment with prometryn for 30 or 60 days.…”

“…The significant drop in the plasma lactate concentration could indicate a decrease in the glycolytic process due to the lower metabolic rate in fish exposed to Gesagard. Similarly, the triazine herbicide metribuzin decreased lactate concentration in blood plasma of common carp after acute exposure (Velisek et al, 2009). On the contrary, lactate concentration was increased in plasma of common carp exposed to terbutryn or prometryn for a long time (Velisek et al, 2011;2013).…”

“…Similarly, plasma TAG levels were unchanged in common carp exposed to simazine (Velisek et al, 2012). Velisek and colleagues (2009) reported diminished TAG levels in plasma of common carp after acute treatment with Sencor (Velisek et al, 2009).…”