The effects of dimethoate on hematological, biochemical parameters, and behavior were investigated in Oncorhynchus mykiss exposed to sublethal concentrations of 0.0735, 0.3675, and 0.7350 mg/l for 5, 15, and 30 days. Significant decrease was determined in erythrocyte and leukocyte counts, hemoglobin, hematocrit, MCV, and MCH, which was pronounced after prolonged exposure indicating the appearance of microcytic hypochromic anemia. There were no prominent changes in thrombocyte and MCHC. The glucose concentration showed an ascending pattern that proved to be positively correlated with duration. The protein concentration declined in higher dimethoate concentrations following 15 and 30 days. Negative and significant correlation was detected between glucose and protein concentrations. The fish showed remarkable behavioral abnormality such as loss of balance, erratic swimming, and convulsion. Present findings revealed that dimethoate exerts its toxic action even in sublethal concentrations and hematological parameters and abnormal behavior may be sensitive indicators to evaluate pesticide intoxication.

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This investigation was conducted to evaluate the effects of clothianidin, a neonicotinoid insecticide, on hepatic oxidative stress biomarkers, biochemical indices of blood serum and liver integrity in juvenile Oncorhynchus mykiss following 7, 14 and 21 days of application to environmentally relevant concentrations of 3, 15 and 30 μg/l. The observed hypertrophy caused elevation in hepatosomatic index, a significant increase in serum glucose and a decrease in tissue protein level with extended period of exposure were determined. The treatment resulted in a marked induction in the activities of antioxidant enzymes which were accompanied with simultaneous elevation in MDA and protein carbonyl level reflecting loss of membrane integrity and protein function. Histopathological examination showed liver injury manifested as hepatocellular degeneration, fibrosis, vacuolation, congestion, necrosis, steatosis and pyknosis proceding with the concentration. The stressful condition triggered hyperglycemic and hypoproteinemic conditions which might be proposed as general adaptive response. Moreover, altered liver histology reveals the hepatotoxic potential of clothianidin via oxidative stress as a common pathological mechanism leading to liver injury.

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