Effects of adenosine 3′,5′-cyclic monophosphate, forskolin, and cholera toxin on hormone production in human term placental cells

Zhou, Fu-zhen; Yuen, Basil Ho; Leung, Peter C.K.

doi:10.1016/0002-9378(87)90296-1

Cited by 16 publications

(4 citation statements)

References 15 publications

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“…As a function of culture time, a parallel study was performed to determine the level of secreted intact hCG and its free a and J3 subunits, using specific monoclonal antibodies and radiometric assay (Bellet et al, 1986;Ozturk et al, 1987). In contrast to data obtained from either term placenta cultured cells (Kliman et al, 1986), or from first trimester explant cultures (Maruo et al, 1987) (Jameson et al, 1987;Zhou et al, 1987;Nulsen et al, 1988). Moreover, the addition of cAMP stimulated the secretion of hPL by cultured cells, in contrast to previous results obtained for term placenta (Zeitler et al, 1983).…”

Section: Discussioncontrasting

confidence: 41%

Characterization and differentiation of human first trimester placenta trophoblastic cells in culture

Dodeur¹,

Malassiné²,

Bellet³

et al. 1990

Reprod. Nutr. Dévelop.

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Summary ― A preparation of highly enriched isolated cytotrophoblasts was obtained from first trimester placenta using dispase incubation of villous tissue at 4 °C, followed by a spontaneous cell release at 37 °C. After 24 h of culture, 90-95% of the cells were immunostained by anticytokeratin antibody, showing their epithelial characteristic. After 48 h of culture, these cells differentiated into syncytiotrophoblast, as shown by optic and electron microscopic study. The secretion of hCG, and of its free a and 13 subunits, and the secretion of hPL were studied as a function of cell culture time. While the level of secreted hCG and its free subunits was stable during 72 h of culture, the hPL level was undetectable during the first 48 h of culture, increasing continuously afterwards. Addition of dibutyryl cAMP from the start or after 96 h of cell culture induced an increase of hCG production and of its free subunits and also stimulated the secretion of hPL. This suggests that these cells maintained the capacity to respond to stimuli which increased intracellular cAMP level. Such a cell culture is of interest in further determining the mechanisms of early gestation involved in the differentiation and growth of placental cytotrophoblasts, and in the regulation of their endocrine functions.

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Section: Discussioncontrasting

confidence: 41%

Characterization and differentiation of human first trimester placenta trophoblastic cells in culture

Dodeur¹,

Malassiné²,

Bellet³

et al. 1990

Reprod. Nutr. Dévelop.

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“…For example, in apparent contrast to earlier studies, others have reported that hCG had either no effect on placental adenylate cyclase (152) and aromatization (141) in minced or homogenized normal placentas, or decreased aromatase activity by normal human term placental cell monolayer cultures (302). Similar observations were recently made by others, in which production of estradiol by human term placental explants (22), or monolayer cell cultures (303), was inhibited by cAMP and unaltered by forskolin and cholera toxin. It appears, therefore, that most work on normal placental tissue is indicative that the aromatase is remarkably resistant to those factors that have been shown effective in other systems.…”

Section: Action Of Campmentioning

confidence: 49%

Placental Steroid Hormone Biosynthesis in Primate Pregnancy*

1990

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Substantial advances in our understanding of placental function have resulted from recent establishment of in vitro approaches, such as cell culture, and application of molecular methods to study placental steroidogenesis. Insight into the processes of placental cell differentiation and hormonal function has been gained from culture of relatively pure preparations of cytotrophoblast. Various factors, e.g. cAMP and peptide growth factors, have been shown to have striking effects on progesterone and estrogen formation by placental tissue under in vitro conditions. Using advanced molecular approaches, the genes governing specific enzymes critical to placental steroidogenesis have been identified. Regulation of the mRNAs encoding specific enzyme peptides and thus expression of the genes by factors, such as cAMP, have been elucidated by Northern analysis and other techniques. It is critical that these contemporary approaches continue to be implemented aggressively to further elucidate placental function. However, it is clear from a survey of the literature, particularly of the past decade, that the vast majority of investigation in the area has been conducted in vitro. It is essential to determine whether the factors that have been observed to regulate placental endocrine function in vitro are operable in vivo. It is only with in vivo study that the dynamics of steroidogenesis and the complex functional relationships between placenta, fetus, and mother will be uncovered and understood. It is increasingly evident that the regulation of placental steroidogenesis involves autocrine and/or paracrine mechanisms, similar to those integral to hormone biosynthesis within other reproductive organs, e.g. ovary and testis. For example, as discussed above, estrogen regulates LDL uptake and P-450scc, and thus apparently is involved in generating substrate for progesterone production within the placenta. Conversely, progesterone has effects on 17 beta-hydroxysteroid oxidoreductase and thus the metabolism of estradiol, while androgens exert marked inhibitory effects on placental progesterone formation, at least in vitro. Not surprisingly, the regulation of placental progesterone and estrogen formation also is multifactorial. Thus, aromatase activity is stimulated synergistically by cAMP and phorbol esters, an effect that is suppressed by peptide growth factors. Therefore, the autocrine/paracrine and multifactorial regulation of hormone biosynthesis that has been relatively well documented in other tissues should be recognized as important in the primate placenta. Finally, the basic mechanisms underlying regulation of steroidogenesis within the fetoplacental unit during primate pregnancy appear similar, in important ways, to those of widely used laboratory animals, such as the rat and rabbit.(ABSTRACT TRUNCATED AT 400 WORDS)

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“…Cyclic AMP and its analogs stimulate the hCG production in the human placental tissue and cell cultures (Handwergeret al 1973;Haninget al 1982;Hilf and Merz 1985;Zeitler, Markoff and Handwerger 1983;Feinman etal. 1986;Zhou, Yuen and Leung 1987;Ulloa-Aguirre et al 1987;Rodway et al 1988). In a preliminary experiment, we found that isoproterenol increased the cAMP content of the cultured human placental cells used in the pre-sent study.…”

Section: Discussionmentioning

confidence: 84%

“…It has been demonstrated that human chorionic gonadotropin (hCG) biosynthesis in placental explants (Handwerger, Barrett, Tyrey and Schomberg 1973;Haning, Choi, Kiggens, Kuzma and Summerville 1982;Hilf and Merz 1985) and placental cells (Zeitler, Markoff and Handwerger 1983;Feinman, Kliman, Caltabiano and Strauss 1986;Zhou, Yuen and Leung 1987;Ulloa-Aguirre, August, Golos, Kao, Sakuragi, Kliman and Strauss 1987;Rodway, Zhou, Benoit, Yuen and Leung 1988) is stimulated by cyclic nucleotides. The placenta has been reported to contain beta adrenergic receptors and catecholamine sensitive adenylate cyclase (Whitsett and Johnson 1979;Whitsett, Johnson, Noguchi, Darovec-Beckerman and Costello 1980).…”

Section: Introductionmentioning

confidence: 99%

Effect of Adrenergic Agonists on Human Chorionic Gonadotropin Release by Human Trophoblast Cells Obtained from First Trimester Placenta

Oike

Iwashita²,

Muraki³

et al. 1990

Horm Metab Res

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The cultured syncytiotrophoblast cells from human first trimester placenta were used to determine the effect of adrenergic agonists on human chorionic gonadotropin (hCG) production in vitro. Beta-adrenergic agonists isoproterenol, ritodrine and isoxsuprine increased the hCG release during the 2 h incubation period, however, alpha-agonists norepinephrine and phenylephrine and a beta 1-agonist dobutamine had no effect. The effect of isoproterenol was blocked by propranolol and butoxamine, but less efficiently by phentolamine and atenolol. These results indicate that placental hCG production can be modulated by stimulation of beta-, possibly beta 2-adrenoceptors but not by alpha-adrenoceptors.

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Effects of adenosine 3′,5′-cyclic monophosphate, forskolin, and cholera toxin on hormone production in human term placental cells

Cited by 16 publications

References 15 publications

Characterization and differentiation of human first trimester placenta trophoblastic cells in culture

Characterization and differentiation of human first trimester placenta trophoblastic cells in culture

Placental Steroid Hormone Biosynthesis in Primate Pregnancy*

Effect of Adrenergic Agonists on Human Chorionic Gonadotropin Release by Human Trophoblast Cells Obtained from First Trimester Placenta

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