Effects of age and mannanoligosaccharides supplementation on production of volatile fatty acids in the caecum of rabbits

Guedes, Cristina; Mourão, José Luís; Silva, Severiano; Gomes, Maria José; Rodrigues, M.A.M.; Pinheiro, Victor

doi:10.1016/j.anifeedsci.2008.10.004

Cited by 18 publications

(13 citation statements)

References 13 publications

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“…The differences among the previous values may also be due to difficulties in the measurement of starch in the ileal contents of the rabbit due to the low quantities of samples available and variable intake pattern of rabbits fed ad libitum, thereby leading to high inter-individual variability (E20%). From 6 to 10 weeks of age, VFA production by rabbits was similar, although there were changes in the fermentation pattern, with a higher proportion of butyrate and lower proportion of acetate being observed in agreement with previous studies (Gidenne et al, 2002;Guedes et al, 2009). …”

Section: Discussionsupporting

confidence: 80%

Effect of substitution of wheat starch by potato starch on the performance, digestive physiology and health of growing rabbits

Pinheiro¹,

Cunha²,

Mourão³

et al. 2013

Animal

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The goal of this research was to study the effect of the substitution of wheat starch by potato starch (PS) on the performance, health and digestion of growing rabbits. Three experimental diets were formulated with 0%, 7% and 14% PS (PS0, PS7 and PS14, respectively) and similar starch contents (22% dry matter basis), proteins and fibre. The three diets were administered to three groups of 48 rabbits from weaning (28 days) to slaughter (70 days), and growth and health measurements were made. Another 10 rabbits per diet (30 rabbits at each age), reared under similar conditions, were slaughtered at 6 to 10 weeks of age, and the digesta were collected to analyse the caecal microbial activity (pH, volatile fatty acids (VFA) levels, fibrolytic activity) and the starch concentration in the ileal digesta. At the same ages, the whole tract digestibility coefficients were measured in 10 other rabbits for each treatment (30 rabbits). The feed intake between 28 and 42 days of age (days) increased by 11% (P , 0.05) in PS0 v. PS14. Over the whole growth period (28 to 70 days), weight gain was similar among diets (40.5 g/day), whereas the feed intake and feed conversion increased (8.5% and 5.2%, respectively; P , 0.05) with the PS14 diet. Mortality and morbidity were not affected by the diets. The starch concentration of the ileal contents increased (P , 0.01) with the addition of PS to the diet (0.39%, 0.77% and 1.08% for diets PS0, PS7 and PS14, respectively). Starch digestibility was 0.8 percentage units higher (99.8% v. 99.0%) with the PS0 diet than the PS14 diet (P 5 0.04). The bacterial cellulolytic activity in the caecum tended to be higher with the PS14 diet (P 5 0.07). The total VFA caecal concentration increased (P , 0.01) only in 6-week-old rabbits with PS7 compared with PS0 (54.7 v. 74.5 mmol/l). Protein digestibility and ileal starch concentration decreased (P , 0.05) with age (6 v. 10 weeks), and hemicelluloses digestibility increased (P , 0.05). At 10 weeks of age, rabbits showed a higher VFA pool (6.25 mol) and proportion of butyrate (15.9%) and a lower proportion of acetate (79.3%), ammonia level (7.5 mmol/l) and C3/C4 ratio (0.31) than at 6 weeks of age. The intake of potato starch had no effect on the performance, caecal microbial activity or digestive health of growing rabbits.

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Section: Discussionsupporting

confidence: 80%

Effect of substitution of wheat starch by potato starch on the performance, digestive physiology and health of growing rabbits

Pinheiro¹,

Cunha²,

Mourão³

et al. 2013

Animal

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“…Several authors investigated the effect of MOS v. antibiotics on in vivo rabbit performance (mortality and growth rates, feed intake), but did not find significant differences (Fonseca et al, 2004;Pinheiro et al, 2004;Mourao et al, 2006). Recently, Guedes et al (2009) found that the addition of 2.0 g/kg MOS to the diet increased volatile fatty acids (VFAs) concentration in the caecum of growing rabbits, but Pinheiro et al (2009) observed that 1.0 g MOS/kg was not able to reduce the negative effect of low fibre diets on rabbit growth performance. However, all the above-mentioned studies (Fonseca et al, 2004;Pinheiro et al, 2004 andMourao et al, 2006;Guedes et al, 2009) showed that the addition of MOS to the diets resulted in a better intestinal integrity and had a protective effect against common pathogens.…”

Section: Introductionmentioning

confidence: 95%

“…Recently, Guedes et al (2009) found that the addition of 2.0 g/kg MOS to the diet increased volatile fatty acids (VFAs) concentration in the caecum of growing rabbits, but Pinheiro et al (2009) observed that 1.0 g MOS/kg was not able to reduce the negative effect of low fibre diets on rabbit growth performance. However, all the above-mentioned studies (Fonseca et al, 2004;Pinheiro et al, 2004 andMourao et al, 2006;Guedes et al, 2009) showed that the addition of MOS to the diets resulted in a better intestinal integrity and had a protective effect against common pathogens. MOS are able to bind the mannose receptors on the type 1 fimbriae of some pathogen bacteria (as E. coli and Salmonella enteritidis) in order to prevent their attachment to intestinal mucosa (Firon et al, 1983;Spring et al, 2000).…”

Section: Introductionmentioning

confidence: 98%

Effect of mannanoligosaccharides supplementation on caecal microbial activity of rabbits

Bovera

Marono

Meo

et al. 2010

Animal

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A total of 200 weaned (35 days) hybrid Hyla rabbits were randomly divided among five groups housed in bicellular cages (20 cages per group). Between 35 and 60 days of age, the groups were submitted to the following treatments: group ANT (positive control) fed a basal diet supplemented with antibiotics (colistin sulphate, 144 mg/kg; tylosin, 100 mg/kg; and oxytetracyclin, 1000 mg/kg); groups MOS_0.5, MOS_1.0 and MOS_1.5 fed the basal diet supplemented with 0.5, 1.0 and 1.5 g/kg mannanoligosaccharides (MOS), respectively; another group fed the basal diet without antibiotics or mannanoligosaccarides supplementation (negative control). Along the trial, an episode of epizootyc rabbit enteropathy occurs so that in the control group mortality rate was very high (78%) and survivor rabbits showed severe symptoms of disease (diarrhoea). Thus, the control group was discarded from the trial. At 60 days of age, samples of caecal content were collected from 10 rabbits per group and used as inocula for an in vitro gas production trial. At the end of fermentation (120 h of incubation), organic matter digestibility (OMd), cumulative gas production, fermentation kinetics, pH, volatile fatty acid (VFA) and NH3 productions were measured. Inoculum from MOS_1.0 rabbits showed the significant higher values of OMd (64.21%, P < 0.05), gas production (262.32 ml/g, P < 0.05), acetate (96.99 mmol/g OM, P < 0.05) and butyrate (26.21 mmol/g OM, P < 0.05) than the other groups. Slight differences were recorded among the groups ANT, MOS_0.5 and MOS_1.5. In addition, branched chain acids, in proportion to total VFAs, were significantly higher in MOS_1.0 inoculum (0.04, P < 0.05). MOS are able to affect fermentation activity of caecal micro-organism, but their activities seem not proportional to their level in the diet.

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“…Prebiotics and in particular mannan‐oligosaccharides (MOS), derived from the outer cell wall of the yeast Saccharomyces cerevisiae , are considered a promising alternative to antibiotics (Kocher, 2006). Several studies (Fonseca et al., 2004; Pinheiro et al., 2004, 2009; Mourao et al., 2006; Guedes et al., 2009) have shown that the addition of MOS to the diets results in a better intestinal integrity and has a protective effect against common pathogens. MOS are able to bind the mannose receptors on the type 1 fimbriae of some pathogen bacteria (such as Escherichia coli and Salmonella enteritidis ) in order to prevent their attachment to intestinal mucosa (Firon et al., 1983; Spring et al., 2000).…”

Section: Introductionmentioning

confidence: 99%

Effect of dietary mannan‐oligosaccharides onin vivoperformance, nutrient digestibility and caecal content characteristics of growing rabbits

Bovera

Lestingi

Marono

et al. 2011

Animal Physiology Nutrition

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To evaluate the effect of mannan-oligosaccharides (MOS) on in vivo performance, nutrient digestibility, fermentation characteristics and caecal microbial populations of rabbits, 144 thirty-five days old hybrid Hyla were equally divided into three groups, one of which was fed the same diet without additives (control group), one with antibiotics (colistin sulphate, 144 mg/kg; tylosin, 100 mg/kg; oxytetracyclin, 1000 mg/kg) and one with MOS (1 g/kg of diet). Mortality rate, live weight, feed intake and feed conversion ratio were recorded up to 62 days of age. At 60 days nutrient digestibility was measured by acid insoluble ash method. The caecal content of 10 rabbits per group was collected at 62 days and analysed for volatile fatty acids production, ammonia content and microbial count. Rabbits from the control group had a significantly (p < 0.01) lower body weight at 62 days (1638.9 g vs. 1779.4 g and 1862.5 g, respectively for the control, MOS and antibiotic groups) while the antibiotic group showed a higher (p < 0.05) feed intake than the control group (127.9 g/day vs. 109.3 g/day). Rabbits from the MOS group had a higher apparent digestibility of cellulose (34.27% vs. 29.61% and 27.49%, respectively for the MOS, control and antibiotic groups) and, as a consequence a higher level of acetate in the caecal content (39.93 mmol/l vs. 34.21 mmol/l and 23.09 mmol/l, respectively for the MOS, control and antibiotic groups). Caecal microflora of the MOS group rabbits also had a higher fermentative activity in respect of protein source, as demonstrated by the higher productions of branched chain fatty acids. MOS and antibiotics significantly reduced the colonies of Coliformis (2.32 vs. 3.20 vs. 2.40 logCFU/g, respectively for the MOS, control and antibiotic groups, p < 0.01). Mannan-oligosaccharides at 1 g/kg of diet can be used as an alternative to antibiotics during the rabbit's growth period.

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Effects of age and mannanoligosaccharides supplementation on production of volatile fatty acids in the caecum of rabbits

Cited by 18 publications

References 13 publications

Effect of substitution of wheat starch by potato starch on the performance, digestive physiology and health of growing rabbits

Effect of substitution of wheat starch by potato starch on the performance, digestive physiology and health of growing rabbits

Effect of mannanoligosaccharides supplementation on caecal microbial activity of rabbits

Effect of dietary mannan‐oligosaccharides onin vivoperformance, nutrient digestibility and caecal content characteristics of growing rabbits

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