The cleaning of string musical instruments is challenging due to the traditional finishing treatments used by the makers. Multilayered coating systems were applied to Western musical instruments, while the Nakdong technique was applied in East Asia. Furthermore, by restorations and performance, dust and grime were overlapped together with polishes, adhesives, and varnishes. Gel cleaning is important in the field of conservation because of the ability to selectively remove chemical and biological degradation products from the surface, minimizing the interactions with the inner layers. In this study, hydrogels based on sodium alginate (SA) and konjac glucomannan (KG) polysaccharides were applied on laboratory mock-ups of East Asian and Western instruments to test their ability to remove synthetic soiling and sweat from the surface. In particular, SA cross-linked with calcium cations and KG cross-linked with borate gels were used. To control the exposure of the cleaning solvent on the surface of mock-ups, the moisture content of the gels was determined. The effectiveness of removing synthetic contaminants was investigated by noninvasive analytical methods. Stereomicroscopy and colorimetry, together with Fourier Transform Infrared (FTIR) spectroscopy in reflection mode and X-Ray Fluorescence (XRF), were used to evaluate the cleaning efficacy. Overall, polysaccharide hydrogels resulted in promising cleaning systems on both smooth and rough surfaces of wood.