Effects of Aging and Food Restriction on the Antioxidant Enzyme Activity of Rat Livers

Gomi, Fujiya; Matsuo, Mitsuyoshi

doi:10.1093/gerona/53a.3.b161

Cited by 47 publications

(31 citation statements)

References 21 publications

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“…Studies measuring 8-hydroxy-2Ј-deoxyguanosine as a marker of DNA oxidative damage have reported that differences emerge between control and CR animals at either 23 mo of age in mice (54) or 30 mo of age in rats (32). Studies of lipid peroxidation in liver have also reported differences between control and CR rats only after the animals reached 30 mo of age (18). Similarly, a study measuring protein carbonyl content in liver from rats reported that significant age-related increases in protein oxidative damage did not occur until the animals were 24 mo old (58).…”

Section: Discussionmentioning

confidence: 99%

Long-term calorie restriction reduces proton leak and hydrogen peroxide production in liver mitochondria

Hagopian¹,

Harper²,

Ram³

et al. 2005

American Journal of Physiology-Endocrinology and Metabolism

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. Longterm calorie restriction reduces proton leak and hydrogen peroxide production in liver mitochondria. Am J Physiol Endocrinol Metab 288: E674 -E684, 2005. First published November 23, 2004 doi: 10.1152/ajpendo.00382.2004.-Calorie restriction (CR) without malnutrition increases maximal life span in diverse species. It has been proposed that reduction in energy expenditure and reactive oxygen species (ROS) production could be a mechanism for life span extension with CR. As a step toward testing this theory, mitochondrial proton leak, H 2O2 production, and markers of oxidative stress were measured in liver from FBNF 1 rats fed control or 40% CR diets for 12 or 18 mo. CR was initiated at 6 mo of age. Proton leak kinetics curves, generated from simultaneous measures of oxygen consumption and membrane potential, indicated a decrease in proton leak after 18 mo of CR, while only a trend toward a proton leak decrease was observed after 12 mo. Significant shifts in phosphorylation and substrate oxidation curves also occurred with CR; however, these changes occurred in concert with the proton leak changes. Metabolic control analysis indicated no difference in the overall pattern of control of the oxidative phosphorylation system between control and CR animals. At 12 mo, no significant differences were observed between groups for H 2O2 production or markers of oxidative stress. However, at 18 mo, protein carbonyl content was lower in CR animals, as was H 2O2 production when mitochondria were respiring on either succinate alone or pyruvate plus malate in the presence of rotenone. These results indicate that long-term CR lowers mitochondrial proton leak and H 2O2 production, and this is consistent with the idea that CR may act by decreasing energy expenditure and ROS production. energy expenditure; aging; oxidative stress; food restriction CALORIE RESTRICTION (CR) without malnutrition is the only intervention that has consistently been shown to increase maximum life span in laboratory rodents (60). In addition to increasing life span in rodents, CR also delays the onset of diabetes, hypertension, and many types of cancer as well as other age-related physiological and pathological conditions (60). This shows that CR not only extends life span but also retards the rate of biological aging. The mechanism(s) responsible for the actions of CR, however, is still unknown.Two theories of aging, the rate of living theory (42) and the free radical theory (24), have been proposed as possible mechanisms for the retardation of aging and diseases by CR (56). However, both of these theories may contain the same mechanism, since mitochondria are central to both energy metabolism and reactive oxygen species (ROS) production. Because both processes contain a common mitochondrial link, it is reasonable to assume that alterations in energy expenditure (defined here as any process that converts food or stored energy to heat and work) could influence ROS production. Thus both theories may be linked by common reduction in mitochondrial ROS pr...

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Section: Discussionmentioning

confidence: 99%

Long-term calorie restriction reduces proton leak and hydrogen peroxide production in liver mitochondria

Hagopian¹,

Harper²,

Ram³

et al. 2005

American Journal of Physiology-Endocrinology and Metabolism

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“…Considering that SOD is a general stress-response factor, one possible but undefined explanation for the increase in SOD in the non-exposed controls might be due to stress arising from their being held in captivity. Another possibility is the starvation-caused alteration of AOE profiles in liver, since food deprivation or starvation has been known to be one of the factors to increase the formation of ROS as reported in both teleosts and mammals (Gomi and Matsuo 1998;Pascual et al 2003;Nam et al 2005). Expression of SOD in aquatic organisms has been known to be significantly affected by a variety of stressors or stimuli, including pathogen infections, exposure to toxic pollutants, nutritional restriction and thermal stress (see Zelko et al 2002).…”

Section: Expression Analysis Of Shark Cu Zn-sod Transcriptsmentioning

confidence: 99%

Characterization of copper, zinc superoxide dismutase from a cartilaginous shark species, Scyliorhinus torazame (Carcharhiniformes)

Nam

Cho

Kim

et al. 2006

Fish Physiol Biochem

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A full-length complementary DNA clone encoding copper, zinc superoxide dismutase (Cu, Zn-SOD), a key antioxidant enzyme, was isolated from the evolutionary lower vertebrate shark species, Scyliorhinus torazame. The shark SOD cDNA comprised 30 bp of 5¢-untranslated region (UTR), 456 bp of a single open reading frame (ORF) encoding 152 amino acids and 401 bp of 3¢-UTR, including consensus polyadenylation signal (AATAAA) and 20 bp of poly-(A+) tail. Deduced amino acid sequence of shark SOD shared relatively high identities with those not only from teleosts (57-72%) but also from other advanced vertebrates (53-65%) with intermediate characteristics. In addition, shark SOD represented unique molecular features that were not seen in other vertebrate SODs. The SOD transcripts were expressed ubiquitously in all tissues examined, including brain, intestine, kidney, liver, muscle and spleen, as determined by reverse transcriptase-polymerase chain reaction (RT-PCR). Acute exposure to cadmium by either injection (0, 1, 2 or 5 mg CdCl 2 kg -1 body weight for 2 days) or immersion (0 or 25 lM Cd for 0, 1, 4 and 7 days) altered hepatic SOD mRNA level, based on RNA blot hybridization and semiquantitative and/or real-time RT-PCR assays. Transcriptional levels of SOD were significantly increased by cadmium exposure generally in a dose-or time-dependent fashion.

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“…In contrast, red crabs collected during C3 had elevated TBARS levels and relatively lower levels of antioxidant enzymes, suggesting an oxidative stress process in red crabs during the autumn of 2006. Decreased antioxidant levels are commonly found under food restriction conditions (Gomi andMatsuo 1998, Bayir et al 2011). Elevated TBARS levels in the absence of increased O 2…”

Section: Discussionmentioning

confidence: 99%

Spatial and temporal variability of oxidative stress indicators in the red crab (Pleuroncodes planipes) from the west coast of the Baja California Peninsula, Mexico

Martínez-Canto

2012

Cienc. Mar.

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ABSTRACT. The red crab Pleuroncodes planipes is a resource with the potential for commercial exploitation. Red crabs have an elevated antioxidant capacity, which is subject to spatial and temporal variability. The objective of this study was to evaluate the variability of the oxidative stress indicators in P. planipes collected on four research cruises off the western coast of the Baja California Peninsula (Mexico). Superoxide radical (O 2•-) production, lipid peroxidation, and antioxidant enzyme activities were quantified in muscle and hepatopancreas of the collected specimens. Significantly higher O 2•-production and antioxidant enzyme activities were found in hepatopancreas than in muscle (P < 0.05), which reflects the specific function of each tissue. Statistically significant differences (P < 0.05) in oxidative stress indicators were found among sampling stations. The results suggest that P. planipes is more susceptible to oxidative damage when sea temperature is warmer, particularly during El Niño conditions.Key words: antioxidants, oxidative damage, oxidative stress, red crab, reactive oxygen species. RESUMEN. La langostillaPleuroncodes planipes es un recurso con potencial de explotación. Las langostillas tienen una elevada capacidad antioxidante, la cual está sujeta a variaciones espaciotemporales. El objetivo de este estudio fue evaluar la variabilidad de los indicadores de estrés oxidativo en P. planipes recolectada en cuatro cruceros de investigación a lo largo de la costa occidental de la península de Baja California (México). Se cuantificó la producción de radical superóxido (O 2•-), la peroxidación de lípidos y la actividad de las enzimas antioxidantes en el hepatopáncreas y músculo de las langostillas recolectadas. Se encontraron niveles significativamente mayores de producción de O 2•-y actividad de las enzimas antioxidantes en el hepatopáncreas que en el músculo (P < 0.05), lo cual refleja la función específica de cada tejido. Se observaron diferencias significativas (P < 0.05) en los indicadores de estrés oxidativo entre las estaciones de muestreo. Los resultados sugieren que P. planipes es más susceptible al estrés oxidativo cuando la temperatura del mar es mayor, particularmente durante condiciones de El Niño.Palabras clave: antioxidantes, daño oxidativo, estrés oxidativo, langostilla, especies reactivas de oxígeno.

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Effects of Aging and Food Restriction on the Antioxidant Enzyme Activity of Rat Livers

Cited by 47 publications

References 21 publications

Long-term calorie restriction reduces proton leak and hydrogen peroxide production in liver mitochondria

Long-term calorie restriction reduces proton leak and hydrogen peroxide production in liver mitochondria

Characterization of copper, zinc superoxide dismutase from a cartilaginous shark species, Scyliorhinus torazame (Carcharhiniformes)

Spatial and temporal variability of oxidative stress indicators in the red crab (Pleuroncodes planipes) from the west coast of the Baja California Peninsula, Mexico

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