In order to study the influence of different aging conditions on the low-temperature crack resistance and water stability of polyester fiber asphalt mixture. Prepare standard Marshall specimens of asphalt mixture with 0.4 % polyester fiber doping, and carry out water immersion Marshall test and low temperature splitting test through indoor asphalt mortar aging, asphalt mixture short-term aging and long-term aging. The results show that: under the three aging conditions, when the water immersion and low temperature time are fixed, with the increase of the aging degree, the water stability and low temperature crack resistance of the asphalt mixture decrease. When the immersion time is 2 h, the stability of asphalt mortar aging and short-term aging decreases by 6.0% and 11.8%, respectively, compared with unaging, but the long-term aging is only 3.6% lower than the short-term aging. When the temperature is -5℃, the split tensile strength of asphalt mortar aged and short-term aged increases by 4.24% and 14.35%, respectively, compared with unaging, while long-term aging only increases 4.18% compared with short-term aging. This indicates that the short-term aging condition has the most significant effect on the water stability and low-temperature crack resistance of polyester fiber asphalt mixes. At the same time, this study established a regression equation between the test temperature and the low temperature evaluation index through quadratic fitting (the correlation coefficient is 0.960-0.998), and the regression relationship can be used to estimate the low temperature evaluation index at different test temperatures.