Effects of Albumin Infusion on Serum Levels of Albumin, Proinflammatory Cytokines (TNF-α, IL-1, and IL-6), CRP, and MMP-8; Tissue Expression of EGRF, ERK1, ERK2, TGF-β, Collagen, and MMP-8; and Wound Healing in Sprague Dawley Rats

Abstract

In this study, we sought to determine the roles of albumin in wound healing, which is infused both pre- and postoperatively in malnourished patients presenting with hypoalbuminemia. For the purposes of the study, we used 25 male Sprague Dawley rats of predetermined weight and age, which were initially maintained in a standard environment and fed the same diet for 7 days prior to being segregated into one of the following five groups: A, control, normal protein feed (20% casein); B, hypoalbuminemia, 25% rat albumin infusion prior to surgery; C, hypoalbuminemia, normal protein feed (20% casein); D, hypoalbuminemia, 25% rat albumin infusion after surgery; and E, hypoalbuminemia, low-protein feed (casein 2%). The animals in all five groups were subjected to four deep incisions in their dorsal muscle fascia. On days 1, 3, 5, and 7 after surgery, ELISA was used to determine serum levels of TNF-α, IL-1, IL-6, CRP, and MMP-8, whereas immunohistochemistry was used to determine the tissue expression of EGFR, ERK1, ERK2, TGF-β, collagen, and MMP-8. Significant reductions in serum levels of TNF-α, IL-1, and CRP were detected in the groups receiving albumin infusion and the high-casein diet (P<0.05). The administration of albumin and a high-casein diet also increased the tissue expression of EGFR, ERK1, ERK2, TGF-β, and collagen and decreased that of MMP-8 relative to the hypoalbuminemia control (P<0.05). We propose that the administration of albumin promoted NF-κB signaling which, in turn, induced the transduction and transcription of factors involved in wound healing. Albumin infusion and dietary proteins play vital roles in accelerating the wound healing process, as they can contribute to correcting the hypoalbuminemic state. These findings provide insights that will contribute to our understanding of wound healing, particularly in malnourished patients.

The preoperative geriatric nutritional risk index (GNRI) is an independent prognostic factor in elderly patients underwent curative resection for colorectal cancer

“…Hypoalbuminemia induces an impaired immune response, and immunity had influence on cancer prognosis 18 . Additionally, a low serum albumin levels was associated with elevated inflammatory cytokines such as interleukin-1, and interleukin-6, tumor necrosis factor-alpha, CRP which may lead to the progression of CRC 19 . Therefore, a low GNRI may reflect impaired tumor immunity which may cause cancer progression.…”

Study on the Correlation Between NF-κB and Central Fatigue

“…Fatigue is associated with inflammatory markers IL6, tumor necrosis factor α (TNFα) and C-reactive protein (Bryleva et al 2017 ; Akcali et al 2017 ; Bower et al 2013 ). NF-κB can induce transcription of cytokines TNFα, IL1β, IL6 and CRP (Utariani et al 2020 ), which play an important role in the occurrence and development of fatigue. Among them, the activation of proinflammatory cytokine IL1β is the key to induce fatigue (Lampa et al 2012 ).…”

Neutrophil Percentage-to-Albumin Ratio: A Good Parameter for the Evaluation of the Severity of Anti-NMDAR Encephalitis at Admission and Prediction of Short-Term Prognosis

“…The strong binding capacity of albumin can reduce the free concentration of toxic metabolites and reduce the pathophysiological damage caused by the poison ( 13 ). Albumin can downregulate the expression and transport of inflammatory factors and reduce the inflammatory cascade reaction ( 14 ). Albumin can also affect the pharmacokinetics of many drugs and thus the efficacy of drugs ( 15 ).…”