Effects of alcohols, povidone-iodine and hydrogen peroxide on biofilms of Staphylococcus epidermidis

Presterl, Elisabeth; Suchomel, Miranda; Eder, Michaela; Reichmann, Sonja; Lassnigg, Andrea; Graninger, Wolfgang; Rotter, M.

doi:10.1093/jac/dkm221

Cited by 113 publications

(89 citation statements)

References 6 publications

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“…Presterl et al compared the efficacy of povidone iodine, alcohol, and H 2 O 2 against Staphylococcus epidermidis biofilm layers and alcohol-H 2 O 2 had a rapid effect on the biofilm layer whereas povidone iodine was comparatively less effective (26).…”

Section: Discussionmentioning

confidence: 99%

The comparison of various disinfectants’ efficacy on Staphylococcus aureus and Pseudomonas aeruginosa biofilm layers

Köse¹,

Yapar²

2017

Turk J Med Sci

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IntroductionA biofilm can be defined as a well-organized microbial community in the extracellular polymeric substance (EPS) that adheres to living or inanimate surfaces (1). Owing to their resistance to antibiotics and antiphagocytic effects, biofilms can frequently cause persistent chronic infections that are difficult to treat. Biofilms are significant causes of morbidity and mortality (2,3). At least 65% of all bacterial infections are associated with biofilm (2,3). Infections such as natural valve endocarditis, otitis media, chronic bacterial prostatitis, cystic fibrosis, and periodontitis develop as a result of biofilms that form on living surfaces. The medical devices on which biofilms can develop include prosthetic heart valves, central venous catheters, urinary catheters, contact lenses, and intrauterine devices (4). Due to the high rates of mortality and morbidity associated with biofilms, several studies have been conducted on antimicrobials and particularly on the effectiveness of antibiotics against biofilms. It has been demonstrated that antibiotics show limited efficacy against biofilms as the biofilm layer persists. For this reason, it should be borne in mind that disinfectants serve as a significant alternative against human mucosa and biofilms on the surfaces of medical device (5). This study aims at investigating the effectiveness of orthophthalaldehyde, peracetic acid, hydrogen peroxide, and sodium hypochlorite against Pseudomonas aeruginosa and Staphylococcus aureus biofilm layers and the live microbial cells on the biofilm layers. Material and methods Bacterial strainsS. aureus (ATCC 6538) and P. aeruginosa (PA01) bacterial strains that are known to produce biofilms were selected (5,6). Bacterial cells stocked at -80 °C were passaged to a tryptic soy agar (TSA) plate (Merck). After being incubated for 24 h at 35-37 °C, a bacterial solution was Background/aim: Because biofilms are resistant to antibiotics and biocides, they usually cause chronic persistent infections, which are arduous to cure and have high mortality and morbidity. Our study aimed to investigate the efficiency of orthophthalaldehyde, peracetic acid, hydrogen peroxide, and sodium hypochlorite on Pseudomonas aeruginosa and Staphylococcus aureus biofilm layers and live microbial cells.Materials and methods: Biofilm layers were determined by crystal violet assay and live microbial cells were determined using a resazurin assay.Results: For Pseudomonas aeruginosa, sodium hypochlorite showed the most influential disinfection because it diminished 83.6% of the biofilm layer and decreased 99.7% of live microbial cells. For Staphylococcus aureus, hydrogen peroxide was determined the most active disinfectant with 80.3% reduction of the biofilm layer. Sodium hypochlorite was also determined to be the most efficient disinfectant with 99.8% reduction of live microbial cells. Sodium hypochlorite was the most influential disinfectant on biofilm layers and live microbial cells of both microorganisms. Conclusion:We concluded that if we use sodi...

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Section: Discussionmentioning

confidence: 99%

The comparison of various disinfectants’ efficacy on Staphylococcus aureus and Pseudomonas aeruginosa biofilm layers

Köse¹,

Yapar²

2017

Turk J Med Sci

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“…[21], yaptıkları çalışmada sadece alkol ve alkol-klorheksidin karışımı içeren In vitro yapılan bu çalışmanın sonuçlarına göre biyofilm oluşturan ve oluşturmayan S. epidermidis suşlarının test edilen antiseptiklere karşı duyarlılığında fark bulunmamıştır.…”

Section: Tablo 2 Kantitatif Süspansiyon Test Sonuçlarıunclassified

Hastanelerde sık kullanılan bazı antiseptiklerin biyofilm oluşturan ve oluşturmayan Staphylococcus epidermidis suşlarına karşı antibakteriyel etkinliğinin araştırılması

GÜRPINAR¹

2017

Ankara Üniversitesi Eczacılık Fakültesi Dergisi

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“…204 Povidone iodine has been found to be less effective than alcohol 60% (v/v) and hydrogen peroxide 3% and 5% on S. epidermidis biofilms. 382 Most iodophor preparations used for hand hygiene contain 7.5-10% povidone-iodine. Formulations with lower concentrations also have good antimicrobial activity, because dilution tends to increase free iodine concentrations.…”

Section: Iodine and Iodophorsmentioning

confidence: 99%

Hand Hygiene

Longtin¹,

Sax²,

Allegranzi³

et al. 2011

N Engl J Med

108

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Effects of alcohols, povidone-iodine and hydrogen peroxide on biofilms of Staphylococcus epidermidis

Abstract: S. epidermidis obtained from infected implants forms thicker biofilms than that of healthy volunteers. Hydrogen peroxide, at a concentration of 3% and 5%, and alcohols rapidly eradicate S. epidermidis biofilms, whereas povidone-iodine is less effective.

Cited by 113 publications

References 6 publications

The comparison of various disinfectants’ efficacy on Staphylococcus aureus and Pseudomonas aeruginosa biofilm layers

The comparison of various disinfectants’ efficacy on Staphylococcus aureus and Pseudomonas aeruginosa biofilm layers

Hastanelerde sık kullanılan bazı antiseptiklerin biyofilm oluşturan ve oluşturmayan Staphylococcus epidermidis suşlarına karşı antibakteriyel etkinliğinin araştırılması

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