Effects of alkaline treatment for fibroblastic adhesion on titanium

Abstract

Background:The surface energy of titanium (Ti) implants is very important when determining hydrophilicity or hydrophobicity, which is vital in osseointegration. The purpose of this study was to determine how Ti plates with an alkaline treatment (NaOH) affect the adhesion and proliferation of human periodontal ligament fibroblasts (HPLF).Materials and Methods:In vitro experimental study was carried out. Type 1 commercially pure Ti plates were analyzed with atomic force microscopy to evaluate surface roughness. The plates were treated ultrasonically with NaOH at 5 M (pH 13.7) for 45 s. HPLF previously established from periodontal tissue was inoculated on the treated Ti plates. The adhered and proliferated viable cell numbers were determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method for 60 min and 24 h, respectively. The data were analyzed using Kruskal–Wallis tests and multiple comparisons of the Mann–Whitney U-test,P value was fixed at 0.05.Results:The mean roughness values equaled 0.04 μm with an almost flat surface and some grooves. The alkaline treatment of Ti plates caused significantly (P < 0.05) more pronounced HPLF adhesion and proliferation compared to untreated Ti plates.Conclusion:The treatment of Ti plates with NaOH enhances cell adhesion and the proliferation of HPLF cells. Clinically, the alkaline treatment of Ti-based implants could be an option to improve and accelerate osseointegration.

Anti-Inflammatory and Antibacterial Activity of the Chitosan/Chlorhexidine Gel Commercial Preparation for Postexodontia Treatment: An In Vitro Study

“…The procedure was performed as previously described for the research group; further information of the technique could be consulted at the reference. 16 Prior approval from the bioethics committee, the tissues for primary culture of human bone cells (HBC), human pulp cells (HPC), and human gingival fibroblast (HGF) were obtained from a 26-year-old patient previously written informed consent. HGF-1 was obtained from ATCC (ATCC CRL-2014).…”

Biofunctionalization of Porous Ti Substrates Coated with Ag Nanoparticles for Potential Antibacterial Behavior

“…In this work, concerning the Ti surface modifications, two alternative hydroxylation treatments were evaluated: a basic treatment, consisting of a conventional chemical etching with a NaOH solution [64,65], and acid treatment by the exposing the Ti surface to concentrated oxidant sulfuric acid, ("piranha solution", H 2 O 2 /H 2 SO 4 ) [66,67]. To perform the attack treatment, either with the basic medium or with acid medium, the Ti samples were soaked in 5 M NaOH at 60 • C for 24 h or with the "piranha solution" at 75 • C (acid solution: 30% H 2 O 2 and 70% H 2 SO 4 ) for 1.5 h, respectively.…”