Effects of alpha-lipoic acid on expression of iron transport and storage proteins in BV-2 microglia cells

Chen, Ping; Li, Fei-Mi; Zhou, Yu‐Fu; Qian, Chunyan; Li, Juan; Jiang, Lirong; Qian, Zhong‐Ming

doi:10.1016/j.pharep.2016.09.011

Cited by 16 publications

(10 citation statements)

References 35 publications

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“…Both iron import and ferritin expression increased following silica exposure of the AMs. Such increased expression of both iron importers and ferritin has been observed previously and is possibly accounted for by temporal disparities in the expression of the proteins. − Particles initially bind cell iron decreasing availability of the metal. Increased import (e.g., transport by divalent metal transporter 1) is proposed to follow, and this would reverse metal deficiency, elevate availability of the metal, and subsequently enhance the expression of storage proteins (e.g., ferritin).…”

Section: Discussionmentioning

confidence: 52%

Quartz Disrupts Iron Homeostasis in Alveolar Macrophages To Impact a Pro-Inflammatory Effect

Ghio

Soukup

Stonehuerner

et al. 2019

Chem. Res. Toxicol.

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The biological response of bronchial epithelial cells to particles is associated with a sequestration of cell metal by the particle surface and a subsequent disruption in host iron homeostasis. The macrophage is the cell type resident in the respiratory tract that is most likely to make initial contact with inhaled particles. We tested the postulates that (1) silica, a prototypical particle, disrupts iron homeostasis in alveolar macrophages (AMs); and (2) the altered iron homeostasis results in both an oxidative stress and pro-inflammatory effects. Human AMs (1.0 × 10 6 /mL) demonstrated an increased import of iron following particle exposure with nonheme iron concentrations of 0.57 ± 0.03, 1.72 ± 0.09, 0.88 ± 0.09, and 3.21 ± 0.11 ppm in cells exposed for 4 h to media, 500 μM ferric ammonium citrate (FAC), 100 μg/mL silica, and both silica and FAC, respectively. Intracellular ferritin concentrations and iron release were similarly increased after AM exposure to FAC and silica. Silica increased oxidant generation by AMs measured using both dichlorofluorescein diacetate fluorescence and reduction of nitroblue tetrazolium salt. Concentrations of interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor-α in macrophage supernatant increased following 100 μg/mL silica exposure for 24 h. Treatment of AMs with 500 μM FAC decreased both oxidant generation and cytokine release associated with silica exposure, supporting a dependence of these effects on sequestration of cell metal by the particle surface. We conclude that (1) silica exposure disrupts iron homeostasis resulting in increased import, accumulation, and release of the metal; and (2) the altered iron homeostasis following silica exposure impacts oxidant generation and pro-inflammatory effects.

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Section: Discussionmentioning

confidence: 52%

Quartz Disrupts Iron Homeostasis in Alveolar Macrophages To Impact a Pro-Inflammatory Effect

Ghio

Soukup

Stonehuerner

et al. 2019

Chem. Res. Toxicol.

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“…Within cells, ferritin serves as the primary complex responsible for iron storage, playing a crucial role in iron homeostasis. Consisting of two subunits, ferritin light polypeptide 1 (FTL1) and ferritin heavy polypeptide 1 (FTH1), this complex forms an intricate structure 47 . It is notable that excess Fe 2+ accumulation within ferritin leads to the formation of an unstable pool of iron.…”

Section: Major Signalling Pathways Regulating Ferroptosismentioning

confidence: 99%

Mechanism of ferroptosis in breast cancer and research progress of natural compounds regulating ferroptosis

Ge,

He,

Zhao

et al. 2023

J Cellular Molecular Medi

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Breast cancer is the most prevalent cancer worldwide and its incidence increases with age, posing a significant threat to women's health globally. Due to the clinical heterogeneity of breast cancer, the majority of patients develop drug resistance and metastasis following treatment. Ferroptosis, a form of programmed cell death dependent on iron, is characterized by the accumulation of lipid peroxides, elevated levels of iron ions and lipid peroxidation. The underlying mechanisms and signalling pathways associated with ferroptosis are intricate and interconnected, involving various proteins and enzymes such as the cystine/glutamate antiporter, glutathione peroxidase 4, ferroptosis inhibitor 1 and dihydroorotate dehydrogenase. Consequently, emerging research suggests that ferroptosis may offer a novel target for breast cancer treatment; however, the mechanisms of ferroptosis in breast cancer urgently require resolution. Additionally, certain natural compounds have been reported to induce ferroptosis, thereby interfering with breast cancer. Therefore, this review not only discusses the molecular mechanisms of multiple signalling pathways that mediate ferroptosis in breast cancer (including metastasis, invasion and proliferation) but also elaborates on the mechanisms by which natural compounds induce ferroptosis in breast cancer. Furthermore, this review summarizes potential compound types that may serve as ferroptosis inducers in future tumour cells, providing lead compounds for the development of ferroptosis‐inducing agents. Last, this review proposes the potential synergy of combining natural compounds with traditional breast cancer drugs in the treatment of breast cancer, thereby suggesting future directions and offering new insights.

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“…Dixon et al experimentally demonstrated that deferoxamine (DFO), an iron chelator, can target the Fenton reaction to reduce ROS production and inhibit ferroptosis (1). Intracellular excess Fe 2+ is usually stored in the form of ferritin, which contains two subunits, FTH1 (ferritin heavy chain 1) and FTL (ferritin light chain) (10). FTH1 functions to store iron.…”

Section: Accumulation Of Ironmentioning

confidence: 99%

Microenvironment immune response induced by tumor ferroptosis—the application of nanomedicine

et al. 2022

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Ferroptosis is a non-apoptotic regulatory form of cell death that has sparked significant interest and research in cancer treatment and certain small chemical inducers have been used in the clinic. These inducers’s weak water solubility, poor targeting, rapid metabolism; and other undesirable characteristics; however, for therapeutic approaches that combine immunotherapy and ferroptosis, challenges such as medication delivery, the complexity of the tumor microenvironment, and immunosuppression remain. The targeted, low toxicity, and efficient distribution benefits of nanotechnology have considerably enhanced the therapeutic efficacy of combining immunotherapy with ferroptosis. This paper describes the distinct mechanism of ferroptosis in tumor therapy and immunotherapy, as well as the application and benefits of nanotechnology in the combination of tumor immunotherapy and ferroptosis.Systematic review registrationhttp://clinicaltrials.gov/, NCT00941070.

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Effects of alpha-lipoic acid on expression of iron transport and storage proteins in BV-2 microglia cells

Abstract: ALA could up-regulate TfR1, DMT1 and ferritin expression when iron is increased outside of the cell, promoting iron deposition into ferritin by increasing cell iron uptake, and then reducing free iron both inside and outside of the cell.

Cited by 16 publications

References 35 publications

Quartz Disrupts Iron Homeostasis in Alveolar Macrophages To Impact a Pro-Inflammatory Effect

Quartz Disrupts Iron Homeostasis in Alveolar Macrophages To Impact a Pro-Inflammatory Effect

Mechanism of ferroptosis in breast cancer and research progress of natural compounds regulating ferroptosis

Microenvironment immune response induced by tumor ferroptosis—the application of nanomedicine

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