Effects of amino acids on microcystin production of the Microcystis aeruginosa

Dai, Ruwei; Liu, Huijuan; Qu, Jiuhui; Zhao, Xu; Hou, Yining

doi:10.1016/j.jhazmat.2008.04.015

Cited by 72 publications

(29 citation statements)

References 28 publications

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“…At the same time, the medium was inspected to certify whether M. aeruginosa was contaminated by extraneous bacterium. Ten milliliter 80% methanolwater solution was added to the cell pellet to extract MC within cyanobacterial cells (Dai et al, 2009). The extraction solution was disrupted using liquid nitrogen freeze-thawing for 20 min and then was put in 50°C water for 20 min to extract MC.…”

Section: Measurement Of Cell Number and Determination Of Mc-lrmentioning

confidence: 99%

Effect of urea on growth and microcystins production of Microcystis aeruginosa

Yan

Wang

et al. 2015

Bioresource Technology

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Section: Measurement Of Cell Number and Determination Of Mc-lrmentioning

confidence: 99%

Effect of urea on growth and microcystins production of Microcystis aeruginosa

Yan

Wang

et al. 2015

Bioresource Technology

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“…The samples were centrifuged, and the algal body was transferred into boiling water for 20 min to extract microcystin (MC). The MC was purified by Solid-Phase Extraction (SPE) and measured by HPLC according to Dai et al (2009). The MC content was calculated by a standard curve, which was drafted from standard microcystin-LR (MC-LR) that was purchased from the Institute of Hydrobiology, Chinese Academy of Sciences (98% purity).…”

Section: P and N Content Measurementsmentioning

confidence: 99%

Analyses of gene expression and physiological changes in Microcystis aeruginosa reveal the phytotoxicities of three environmental pollutants

et al. 2012

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When the concentrations of ampicillin (Amp), atrazine (Atr) and cadmium chloride (Cd) reach excessive quantities, they become toxic to aquatic organisms. Due to the acceleration of the industrialization and the intensification of human activities, the incidence and concentrations of these types of pollutants in aquatic systems are increasing. The primary purpose of this study was to evaluate the short-term effects of Amp, Atr and Cd on the physiological indices and gene expression levels in Microcystis aeruginosa. These three pollutants significantly induced antioxidant activity but continuously accelerated the cellular oxidative damage in microalgae, which suggests an imbalance between the oxidant and the antioxidant systems. Amp, Atr and Cd also decreased the transcription of psaB, psbD1 and rbcL; the lowest transcription of these genes was only 38.1, 23.7 and 7% of the control, respectively. These three pollutants affected nitrogen (N) and phosphorous (P) uptake by inhibiting the transcription of N or P absorbing and transporting related genes, and they down regulated the transcription of microcystin-related genes, which caused a decrease of microcystin levels; and the lowest level of microcystin was only 42.4% of the control. Our results suggest that these pollutants may cause pleiotropic effects on algal growth and physiological and biochemical reactions, and they may even affect secondary metabolic processes.

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“…Microcystis aeruginosa are widely recognized as one of the most common bloom-forming cyanobacteria in Chinese eutrophic freshwater bodies, such as Taihu Lake (Xie and Liu 2001) and Dianchi Lake (Dai et al 2009), which are both highly impacted by urea loss from agriculture (Wu et al 2010;Yang et al 2011). However, the relationship between the loss of urea-fertilizer and the occurrence of Microcystis blooms is still unknown.…”

mentioning

confidence: 99%

“…The most commonly studied MCs are microcystin-LR (MC-LR) (2: Leu, 4: Arg) and microcystin-RR (MC-RR) (2: Arg, 4: Arg) (Park and Watanabe 1995). MCs can threaten ecosystem integrity as well as public health (Dai et al 2009) and may play a key role in the persistence of cyanobacterial colonies and the dominance of Microcystis (Gan et al 2012). In this context, the present study aims at assessing the ability of M. aeruginosa to utilize urea as a sole N source employing growth, photosynthetic activity as well as toxin production as physiological endpoints.…”

mentioning

confidence: 99%

Effects of Fertilizer-Urea on Growth, Photosynthetic Activity and Microcystins Production of Microcystis aeruginosa Isolated from Dianchi Lake

Huang

2014

Bull Environ Contam Toxicol

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Urea is the most frequently applied nitrogen (N) fertilizer in agriculture, while its loss is assumed triggering algal blooms in adjacent water bodies. In this context the present study assessed the growth, photosynthetic activity as well as toxin production of Microcystis aeruginosa at different urea concentrations (0.125, 1.25, 12.5, 250 and 2,500 mg/L) using BG11 (containing 250 mg/L NO 3 --N) as control. The results showed for all endpoints that M. aeruginosa is capable of using urea as N source: the two highest urea treatments delivered comparable values like the control. Low urea concentrations (0.125 and 1.25 mg/L), which were comparable to environmental urea levels, did not sustainably promote the growth, photosynthesis and toxin production of the test species. While, in certain microenvironments urea might potentially reach the concentrations that may affect M. aeruginosa.

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Effects of amino acids on microcystin production of the Microcystis aeruginosa

Cited by 72 publications

References 28 publications

Effect of urea on growth and microcystins production of Microcystis aeruginosa

Effect of urea on growth and microcystins production of Microcystis aeruginosa

Analyses of gene expression and physiological changes in Microcystis aeruginosa reveal the phytotoxicities of three environmental pollutants

Effects of Fertilizer-Urea on Growth, Photosynthetic Activity and Microcystins Production of Microcystis aeruginosa Isolated from Dianchi Lake

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