Effects of androgen on immunohistochemical localization of androgen receptor and Connexin 43 in mouse ovary

Yang, Mei; Li, Jianhua; An, Yulin; Zhang, Shuiwen

doi:10.1016/j.tice.2015.06.003

Cited by 23 publications

(18 citation statements)

References 37 publications

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“…In Cx37 or Cx43 deficient mice, folliculogenesis is arrested at the early stage, where oocytes fail to reach the meiotic stage [22,23]. Furthermore, Cx43 expression is increased during follicular development and decreased during follicular atresia [24]. Cx37 is a predominant connexin that makes up gap junctions in oocytes.…”

Section: Wwwbjbmsorgmentioning

confidence: 99%

“…Of the 10 eligible articles, three were in vitro studies that involved culture of oocytes derived from the Institute of Cancer Research (ICR) mice [29], Sprague Dawley rats [30], or humans [31]. Six articles were in vivo studies involving mice [24] or pigs [32][33][34][35][36]. One article combined both in vitro and in vivo studies on Sprague Dawley rats [37].…”

Section: Study Characteristicsmentioning

confidence: 99%

“…All studies measured the expression of Cx43 as the parameter, except for Zhang et al [29] who assessed Cx37 expression. Testosterone was used as treatment in three studies [24,29,30], one study used DHT [31], and one study used androstenedione [37]. The remaining five studies used the AR blocker flutamide as treatment [32][33][34][35][36].…”

Section: Study Characteristicsmentioning

confidence: 99%

“…Only two in vivo studies involving direct androgen treatment were included in this review, showing contradictory results. Yang et al [24] reported that testosterone reduced Cx43 expression in the ovaries of mice, while Talhouk et al [37] showed that androstenedione increased Cx43 expression in the ovaries of rats. Moreover, in the Yang study, while Cx43 expression in the ovaries was reduced by testosterone treatment, the AR expression was upregulated [24].…”

Section: In Vivo Studiesmentioning

confidence: 99%

“…Yang et al [24] reported that testosterone reduced Cx43 expression in the ovaries of mice, while Talhouk et al [37] showed that androstenedione increased Cx43 expression in the ovaries of rats. Moreover, in the Yang study, while Cx43 expression in the ovaries was reduced by testosterone treatment, the AR expression was upregulated [24]. This indicates that the effect of testosterone to downregulate the expression of Cx43 is mediated through the AR.…”

Section: In Vivo Studiesmentioning

confidence: 99%

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Androgen effect on connexin expression in the mammalian female reproductive system: a systematic review

Kamal

Ibrahim

Mokhtar

2019

Bosn J of Basic Med Sci

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The functions of androgen and connexin in the mammalian female reproductive system are suggested to be related. Previous research has shown that androgen affects connexin expression and localization in the female reproductive system, altering its function. However, no definitive conclusion on their cause-effect relationship has been drawn yet. In addition, a high prevalence of women with polycystic ovary syndrome (PCOS), who are characterized by elevated androgen levels and failure of ovulation, has prompted the studies on the relationship between androgen and connexin in the ovaries. This systematic review aims to investigate the effect of androgen on connexin expression in the mammalian female reproductive system. The literature search was conducted using the MEDLINE, EBSCOhost, and Scopus databases and the following keywords: “androgen” or “testosterone” or “androgen blocker” or “anti-androgen” or “androstenedione” or “dehydroepiandrosterone” or “flutamide AND connexin” or “gap junction” or “cell junction”. We only considered in vitro and in vivo studies that involved treatment by androgen or androgen receptor blockers and measured connexin expression as one of the parameters. Our review showed that the exposure to androgen or androgen blocker affects connexin expression but not its localization in the mammalian ovary. However, it is not clear whether androgen downregulates or upregulates connexin expression. Additionally, it is inconclusive whether the upregulation of connexin positively contributes to the ovarian function.

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Section: Wwwbjbmsorgmentioning

confidence: 99%

Section: Study Characteristicsmentioning

confidence: 99%

Section: Study Characteristicsmentioning

confidence: 99%

Section: In Vivo Studiesmentioning

confidence: 99%

Section: In Vivo Studiesmentioning

confidence: 99%

See 3 more Smart Citations

Androgen effect on connexin expression in the mammalian female reproductive system: a systematic review

Kamal

Ibrahim

Mokhtar

2019

Bosn J of Basic Med Sci

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Gap junctions in polycystic ovary syndrome: Implications for follicular arrest

Zhu,

2024

Developmental Dynamics

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Gap junctions are specialized intercellular conduits that provide a direct pathway between neighboring cells, which are involved in numerous physiological processes, such as cellular differentiation, cell growth, and metabolic coordination. The effect of gap junctional hemichannels in folliculogenesis is particularly obvious, and the down‐regulation of connexins is related to abnormal follicle growth. Polycystic ovary syndrome (PCOS) is a ubiquitous endocrine disorder of the reproductive system, affecting the fertility of adult women due to anovulation. Exciting evidence shows that gap junction is involved in the pathological process related to PCOS and affects the development of follicles in women with PCOS. In this review, we examine the expression of connexins in follicular cells of PCOS and figure out whether such communication could have consequences for PCOS women. While along with results from clinical and related animal studies, we summarize the mechanism of connexins involved in the pathogenesis of PCOS.

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Ovarian follicular dynamics after aromatizable or non aromatizable neonatal androgenization

Anesetti

Chávez-Genaro

2016

J Mol Hist

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The effects of neonatal testosterone or dihydrotestosterone exposure on ovarian follicular dynamics were analysed at prepubertal, pubertal or adult age in Wistar rats. Both androgens induced a transitory increase on follicular endowment that was partially corrected at puberty. At adult age testosterone prevented ovulation, without significant modifications on follicular dynamics. An increased number of cystic structures were observed from puberty to adult age. However, ovaries of rats treated with dihydrotestosterone showed follicles with evident morphological alterations in granulosa and thecal layers although several corpora lutea were observed. A significant increase in preantral follicles and few cystic structures were detected at advanced adulthood. The size of cyst increased with age. No immunohistochemical changes on growth factors or enzymes related to steroidogenesis in growing follicles were obvious in any group. In both androgenized groups, cysts shared immunohistochemical characteristics exhibited by preovulatory follicles but they were unable to ovulate spontaneously. Our results provide an insight into the role of different androgens in female reproductive system development, indicating a direct effect of dihydrotestosterone on ovarian tissues whereas a central effect would be the main feature of neonatal testosterone exposure. Heterogeneous clinical manifestations seen in pathologies such as polycystic ovary syndrome among women could be associated with subtle hormonal changes during follicular population development.

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Effects of androgen on immunohistochemical localization of androgen receptor and Connexin 43 in mouse ovary

Cited by 23 publications

References 37 publications

Androgen effect on connexin expression in the mammalian female reproductive system: a systematic review

Androgen effect on connexin expression in the mammalian female reproductive system: a systematic review

Gap junctions in polycystic ovary syndrome: Implications for follicular arrest

Ovarian follicular dynamics after aromatizable or non aromatizable neonatal androgenization

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