Effects of anion channel blockers on xylem nitrate transport in barley seedlings

Kawachi, Tahei; Nishijo, Chizuru; Fujikake, Hiroyuki; Abdel-Latif, Salwa; Ohtake, Norikuni; Sueyoshi, Kuni; Ohyama, Takuji; Shigeta-Ishioka, Noriko; Watanabe, Satoshi; Osa, A.; Sekine, T.; Matsuhashi, Sachiko; Ito, T.; Mizuniwa, Chizuko; Kume, Tamikazu; Hashimoto, Shouji; Uchida, Hiroshi; Tsuji, Atsunori

doi:10.1080/00380768.2002.10409200

Cited by 10 publications

(3 citation statements)

References 35 publications

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“…To minimize secondary effects (e.g. toxicity) of the anion channel blockers, we added each chemical at concentrations of 50 mM, which were similar to those used in other studies (Ryan et al 1995, Kawachi et al 2002. Among the four blockers, NA and A-9-C showed strong inhibitory effects on citrate excretion (60-70%; Fig.…”

Section: Effects Of Anion Channel Blockers On Citrate Excretionmentioning

confidence: 99%

Characterization of Citrate Transport through the Plasma Membrane in a Carrot Mutant Cell Line with Enhanced Citrate Excretion

Ohno

Koyama

Hara

2003

Plant and Cell Physiology

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;The superior ability of citrate excretion in a carrot (Daucus carota L.) mutant cell line, namely IPG (insoluble phosphate grower) [Takita et al. (1999a) Plant Cell Physiol. 40: 489] cells has been characterized in terms of citrate transport at the plasma membrane. IPG cells released about a 20-fold increase in citrate in comparison with malate, while the concentration of malate was only 35% lower than that of citrate in the cell sap. Citrate excretion was sensitive to anion channel blockers, such as niflumic acid and anthracene-9-carboxylic acid. These results indicate that IPG cells release citrate through the plasma membrane using citrate specific anion channels. The rate of citrate release from IPG cells was not affected by the concentration of aluminum (0 and 50 mM), soluble P i (0 or 2 mM) and the pH (4.5-5.6) of the medium, suggesting that anion channels would not be regulated by such external conditions. Citrate excretion correlated with the H + efflux, possibly from the action of H + -ATPase on the plasma membrane. The activity of plasma membrane H + -ATPase was about three times higher in IPG cells than in wild-type cells, and might be involved in the high citrate excretion ability.Keywords: Aluminum phosphate -Anion channel -Citrate excretion -Daucus carota -H + -ATPase.Abbreviations: A-9-C, anthracene-9-carboxylic acid; DIDS, 4,4¢-diisothiocyanostilbene-2,2¢-disulfonic acid; EA, ethacrynic acid; IPG, insoluble phosphate grower; NA, niflumic acid; WT, wild-type. IntroductionOrganic acid excretion from roots, which plays various roles in efficient nutrient uptake (e.g. phosphate, Schactman et al. 1998; iron, Hirsch and Sussman 1999) and the alleviation of metal toxicities (e.g. aluminum, Miyasaka et al. 1991, Ma 2000, is thought to be one of the most important plant traits for adaptation to problem soils. For example, certain plant species showing an aluminum tolerance or a high P i -acquisition ability, such as Cassia tora L. (Ma et al. 1997) and lupin (Gardner et al. 1981), release large amounts of organic acids from their roots. Genetic manipulation may be one approach to introduce such traits into crop plants. In fact, transgenic tobacco (de la Fuente et al. 1997) and Arabidopsis (Koyama et al. 2000), with overexpression of the citrate synthase gene from bacteria and plants, increased citrate excretion from the roots. On the other hand, overexpression of a malate dehydrogenase in alfalfa (Tesfaye et al. 2001) causes enhanced citrate excretion from the roots, and in turn improves growth in either the presence of Al in the growing medium or with an Al-phosphate as the sole phosphate source. These results indicate that the modification of organic acid metabolisms is a target for improving organic acid excretion from the roots of crop plants. Additionally, several studies have suggested that organic acid transport through plasma membranes is another important factor for attaining high organic acid excretion abilities from roots (Ryan et al. 1995).There are two distinct patterns of organic acid excret...

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Section: Effects Of Anion Channel Blockers On Citrate Excretionmentioning

confidence: 99%

Characterization of Citrate Transport through the Plasma Membrane in a Carrot Mutant Cell Line with Enhanced Citrate Excretion

Ohno

Koyama

Hara

2003

Plant and Cell Physiology

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“…As X‐QUAC is the most frequently observed in barley and maize steles, this channel is likely to be the most influential (Kohler & Raschke, 2000; Gilliham & Tester, 2005). Consistent with this, DIDS (a potent inhibitor of X‐QUAC in barley; Kohler et al ., 2002) significantly reduces anion loading into the xylem of intact excised barley roots (Kawachi et al ., 2002). Furthermore, water stress and ABA treatment result in an accumulation of solutes in maize roots (Cram & Pitman, 1972; Sharp & Davies, 1979); this is entirely consistent with the inhibition of anion loading into the xylem vessels via the downregulation of X‐QUAC in maize root stelar protoplasts by ABA and water stress (Gilliham & Tester, 2005).…”

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confidence: 99%

Plasma membrane anion channels in higher plants and their putative functions in roots

2006

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Contents Summary 647 Introduction 648 Overview of higher plant plasma membrane anion channels 648 Anion channels in higher plant roots 655 Conclusions and prospects 661 Acknowledgements 662 References 662 Summary Recent years have seen considerable progress in identifying anion channel activities in higher plant cells. This review outlines the functional properties of plasma membrane anion channels in plant cells and discusses their likely roles in root function. Plant anion channels can be grouped according to their voltage dependence and kinetics: (1) depolarization‐activated anion channels which mediate either anion efflux (R and S types) or anion influx (outwardly rectifying type); (2) hyperpolarization‐activated anion channels which mediate anion efflux, and (3) anion channels activated by light or membrane stretch. These types of anion channel are apparent in root cells where they may function in anion homeostasis, membrane stabilization, osmoregulation, boron tolerance and regulation of passive salt loading into the xylem vessels. In addition, roots possess anion channels exhibiting unique properties which are consistent with them having specialized functions in root physiology. Most notable are the organic anion selective channels, which are regulated by extracellular Al3+ or the phosphate status of the plant. Finally, although the molecular identities of plant anion channels remain elusive, the diverse electrophysiological properties of plant anion channels suggest that large and diverse multigene families probably encode these channels.

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“…located midway on the root, whereas uptake of the tracer moved to the aerial part. Compared to our previous method of a visualization of 13 N translocation using PETIS (Kawachi et al . 2002; Kiyomiya et al .…”

Section: Resultsmentioning

confidence: 99%

Analysis of NO₃interception of the parasitic angiospermOrobanchespp. using a positron-emitting tracer imaging system and¹³NO⁻₃: A new method for the visualization and quantitative analysis of the NO₃interception ratio

Kawachi

Fujimaki

Sakamoto

et al. 2008

Soil Science and Plant Nutrition

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The root parasitic plants Orobanche spp. (broomrapes) seriously affect agricultural production. A visualization and quantitative analytical method for the interception of nutrients was established using a positron-emitting tracer imaging system and 13 NO 3 -. By using this analytical method that involves volume normalization with 18 Fimages, the nitrogen nutrient interception ratio of the Orobanche spp. was calculated to be 73.6 ± 3.9% in a host-parasite system of red clover (Trifolium pratense L.).

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Effects of anion channel blockers on xylem nitrate transport in barley seedlings

Cited by 10 publications

References 35 publications

Characterization of Citrate Transport through the Plasma Membrane in a Carrot Mutant Cell Line with Enhanced Citrate Excretion

Characterization of Citrate Transport through the Plasma Membrane in a Carrot Mutant Cell Line with Enhanced Citrate Excretion

Plasma membrane anion channels in higher plants and their putative functions in roots

Analysis of NO₃interception of the parasitic angiospermOrobanchespp. using a positron-emitting tracer imaging system and¹³NO⁻₃: A new method for the visualization and quantitative analysis of the NO₃interception ratio

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Effects of anion channel blockers on xylem nitrate transport in barley seedlings

Cited by 10 publications

References 35 publications

Characterization of Citrate Transport through the Plasma Membrane in a Carrot Mutant Cell Line with Enhanced Citrate Excretion

Characterization of Citrate Transport through the Plasma Membrane in a Carrot Mutant Cell Line with Enhanced Citrate Excretion

Plasma membrane anion channels in higher plants and their putative functions in roots

Analysis of NO3interception of the parasitic angiospermOrobanchespp. using a positron-emitting tracer imaging system and13NO−3: A new method for the visualization and quantitative analysis of the NO3interception ratio

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Analysis of NO₃interception of the parasitic angiospermOrobanchespp. using a positron-emitting tracer imaging system and¹³NO⁻₃: A new method for the visualization and quantitative analysis of the NO₃interception ratio