Effects of anterior thalamic nuclei stimulation on gene expression in a rat model of temporal lobe epilepsy

Liu, Defeng; Chen, Ying; Zhu, Guanyu; Wang, Xiu; Jiang, Yin; Liu, Huan-Guang; Zhang, Jianguo

doi:10.1007/s13760-019-01240-1

Cited by 7 publications

(2 citation statements)

References 36 publications

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“…Their results showed that this microRNA plays a role in the development of epilepsy by regulating the inflammation or apoptosis [15]. Consistently, the downregulation of miR-15a-5p expression levels in serum have been reported in children with TLE by [44]. They also investigated the effect of the over-expression of this microRNA on the cell viability and apoptosis of hippocampal cells and their results proved that high levels of miR-15a-5p caused the inhibition of apoptosis and enhanced the cell viability.…”

Section: Discussionmentioning

confidence: 60%

Differential Expression Analysis of miRNAs and mRNAs in Epilepsy Uncovers Potential Biomarkers

El Abed,

Baraket,

Nyamari

et al. 2023

Preprint

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Epilepsy is a neurological disease defined by episodes of synchronous convulsions. Recently, miRNAs have been proven as promising biomarkers for multiple ailments like tumors and neurodegenerative disorders; their role in epilepsy is still unclear. This study aimed to understand the involvement of miRNAs in the disease and to detect the potential biomarkers for the treatment of epilepsy. RNA transcripts, and miRNA from brain tissue and plasma small extracellular vesicle samples of epileptogenic patients from 6 different studies downloaded from the NCBI sequence read archive (SRA) were analyzed with particular interest in genes that might be involved in epilepsy. Alignment of transcripts to hg38 was done using HISAT2 and the raw counts were generated using HTseq-count. miRNA genes were identified using miRDeep2. EdgeR and GEO2 were used to identify DEGs for both mRNA and miRNA datasets. Finally, TargetScan web tool was used to predict potentially significantly expressed mRNA target genes using the identified miRNA genes. Analysis of these datasets revealed target genes in epilepsy and their associated miRNAs. SIX4 and KCTD7 were under-expressed in epileptogenic zones of the brain compared to the irritative zone. CABP1, SLC20A1 and SLC35G1 were under-expressed in brain tissues. Hsa-miR-27a-3p was identified as a regulator of CABP1 expression, hsa-let-7b-5p regulates SLC20A1 while hsa-miR-15a-5p and hsa-miR-195-5p are regulators for SLC35G1. These observations highlight the importance of miRNAs as novel biomarkers of epilepsy. Understanding and controlling these regulatory interactions may help to define potential therapies for epilepsy. This would also help to better understand miRNA-mediated gene regulation in epilepsy.

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Section: Discussionmentioning

confidence: 60%

Differential Expression Analysis of miRNAs and mRNAs in Epilepsy Uncovers Potential Biomarkers

El Abed,

Baraket,

Nyamari

et al. 2023

Preprint

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show abstract

“…Although high frequency stimulation of the ANT has been widely used to treat partial seizures [7] there are few studies on ANT DBSrelated mechanisms of action. The variety of possible mechanisms that culminate with seizure reduction include reduction of cell death [46], mossy fiber sprouting [47], apoptosis and inflammation [29,48], altered gene expression in calcium signaling pathways and glutamatergic synapses [49], enhancement of local nonselective norepinephrine and serotonin release [50,51], local decreased glutamate and aspartate release with concomitant increase of GABA [52], and increased levels of adenosine in the hippocampus [22,28]. In line with those mechanisms, our current results demonstrate that ANT-DBS resulted in hippocampal neuroprotection and reduced expression of ADK.…”

Section: Discussionmentioning

confidence: 99%

Deep brain stimulation of the anterior thalamus attenuates PTZ kindling with concomitant reduction of adenosine kinase expression in rats

et al. 2022

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Brain stimulation treatments in epilepsy: Basic mechanisms and clinical advances

Foutz

Wong

2022

Biomedical Journal

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Effects of anterior thalamic nuclei stimulation on gene expression in a rat model of temporal lobe epilepsy

Cited by 7 publications

References 36 publications

Differential Expression Analysis of miRNAs and mRNAs in Epilepsy Uncovers Potential Biomarkers

Differential Expression Analysis of miRNAs and mRNAs in Epilepsy Uncovers Potential Biomarkers

Deep brain stimulation of the anterior thalamus attenuates PTZ kindling with concomitant reduction of adenosine kinase expression in rats

Brain stimulation treatments in epilepsy: Basic mechanisms and clinical advances

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