Effects of Anti-Lobster Nerve Antibody on Membrane Potentials of the Giant Axon of Palinurus Vulgaris

Effect of Specific Antibodies on the Excitability of Internally Perfused Squid Axons

“…Electrical recording in these cases was accomplished over the same type of electrode as was used in the perfusion experiments, inserted through the distal end of the axon. In this way possible penetration of antibodies into the axon, as might occur if the axons were impaled by a microelectrode, was avoided (2). In six of these experiments in which antiaxoplasm antibodies were tested, no effects were apparent.…”

“…axons were impaled by a microelectrode, was avoided (2). In six of these experiments in which antiaxoplasm antibodies were tested, no effects were apparent.…”

“…These authors also reported that lobster giant axons become inexcitable when immersed in a medium containing antibodies against the lobster ventral cord. T h e y conclude that the antibodies exerted their effects from the inner side of the membrane, penetration being achieved by leakage at the microelectrode impalement site (2).…”

“…Interest in the effects of specific immune sera on nervous system function has been stimulated by the recent studies of L. Mihailovid and collaborators (1,2). T h e y have shown that antibodies prepared against tissue from well-defined anatomical areas of the brain will alter the electrophysiological properties of that area when injected diffusely into the central nervous system, in certain instances showing a marked specificity (1).…”