Effects of Antimitotic Agents on Haploid Plant Production from Unpollinated Ovules of Sugar Beet (<i>Beta Vulgaris</i>L.)

Gürel, Songül; Gürel, Ekrem; Kaya, Zeki; Erdal, Mustafa; Güler, Emrah

doi:10.1080/13102818.2003.10817065

Cited by 9 publications

(4 citation statements)

References 16 publications

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“…Buna ek olarak, oksin ilavesinin faydalı olmadığı, %3 sukroz içeren bir MS ortamı kullanılarak 1.0 mg/L NAA uygulanmasıyla sürgünlerde köklenmenin de sağlanabildiği belirlenmiştir [18]. Türkiye'de şeker pancarı ıslahında; bitki doku kültürü çalışmalarıyla genetik materyalin klonla [19][20][21][22][23][24][25][26][27], hücre kültürüyle [28], kallus kültürüyle [15], ovül kültürüyle [21,29] ve protoplast kültürüyle [30], çoğaltılmasına yönelik bir takım çalışmalar yürütülmüştür. Bitki doku kültürü çalışmalarıyla; aynı genetik yapıya sahip birçok birey; hızlı ve sağlıklı olarak çoğaltılabilmektedir [15,[19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37].…”

Section: Introductionunclassified

“…Türkiye'de şeker pancarı ıslahında; bitki doku kültürü çalışmalarıyla genetik materyalin klonla [19][20][21][22][23][24][25][26][27], hücre kültürüyle [28], kallus kültürüyle [15], ovül kültürüyle [21,29] ve protoplast kültürüyle [30], çoğaltılmasına yönelik bir takım çalışmalar yürütülmüştür. Bitki doku kültürü çalışmalarıyla; aynı genetik yapıya sahip birçok birey; hızlı ve sağlıklı olarak çoğaltılabilmektedir [15,[19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37]. Ancak, ıslah programına dâhil edilen önemli kültür bitkilerine yakın akraba doğal türlerinin bitki doku kültürü teknikleriyle çoğaltılmasına yönelik en uygun koşulların belirlenmesi ve tespiti için yeni çalışmalara ihtiyaç duyulmaktadır.…”

Section: Introductionunclassified

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Kıyı Pancarı (Beta vulgaris subsp. maritima (L.) Arcang) Türünün In-vitro Şartlarda Mikro Çoğaltımı

KOLÇAK>¹,

Sarihan

2022

Uşak Üniversitesi Fen Ve Doğa Bilimleri Dergisi

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Bu çalışmada kıyı pancarı (Beta vulgaris subsp. maritima (L.) Arcang) bitkisinin in-vitro koşullarda farklı eksplantlarının değişik bitki büyüme düzenleyicisi (Kinetin ve Indol bütirik asit) kombinasyonlarında rejenerasyon yeteneklerinin belirlenmesi amaçlanmıştır. Kinetin (KIN) ve Indol-3-bütirik asit (İBA)’in farklı dozları (0; 0,5; 1,0; 1,5 ve 2,0 mg/L) ile hazırlanan besi ortamlarında hipokotil, boğum ve kotiledon eksplantlarının rejenerasyon yetenekleri belirlenmiştir. Çalışmada kallus oluşturma oranı (%), sürgün oluşturma oranı (%), köklenme oranı (%), eksplant başına sürgün sayısı (adet), kök sayısı (adet) ve en uzun kök uzunluğu (mm) gibi karakterlerde ölçümler yapılmıştır. Elde edilen sonuçlara göre en iyi sürgün oluşturma oranı (%100) boğum eksplantlarından elde edilmiştir. Bu eksplantın tüm uygulamalarında aynı sonuç elde edilmiştir. Bu eksplantın sürgünlerinin kök oluşum oranı kinetinsiz ortamda, 0,5, 1,5 ve 2,0 mg/L İBA dozlarının uygulandığı ortamlarda %100 olarak gerçekleşmiştir. Elde edilen sonuçlara göre boğum eksplantında 0,0 mg/L KİN + 1,5 mg/L İBA dozunun bu bitki türünün in-vitro çoğaltılmasında en etkili şekilde kullanılabilecek bitki büyüme düzenleyicileri kombinasyonu olduğu belirlenmiştir. Bu bitki türünün hızlı çoğaltılmasında boğum eksplantının çok uygun bir şekilde bitkinin hızlı çoğaltılmasında kullanılabileceği tespit edilmiştir.

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Section: Introductionunclassified

Kıyı Pancarı (Beta vulgaris subsp. maritima (L.) Arcang) Türünün In-vitro Şartlarda Mikro Çoğaltımı

KOLÇAK>¹,

Sarihan

2022

Uşak Üniversitesi Fen Ve Doğa Bilimleri Dergisi

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“…There, basic investigations in different fields of biotechnology including working out of methods of sugar beet breeding for resistance to stresses are concentrated. Using biotechnologies, sugar beet breeding materials were obtained in Germany, Turkey (Gürel et al, 2003), Poland (Tomaszewska-Sowa, 2012, Bulgaria (Kikindonov et al, 2016), and so on. Technological indices of the breeding material produced with the help of sugar beet doubled haploids in Byelorussia, which demonstrate success in breeding at (or above) the level of usual hybrids, are of interest.…”

Section: Introductionmentioning

confidence: 99%

Biotechnological methods as a tool for efficient sugar beet breeding

Zhuzhzhalova¹,

Колесникова²,

Васильченко³

et al. 2020

Vestn. VOGiS

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Here we consider aspects of the application of biotechnological methods to rapid creation, propagation, and maintenance of plants with improved or new traits in sugar beet breeding. The results of the works carried out in these fields by the Federal State Budgetary Scientific Institution “The A.L. Mazlumov All-Russia Research Institute of Sugar Beet” are reviewed. A close association between morphological and physiological changes in in vitro cultured organs and tissues, on the one hand, and breeding traits, on the other hand, which allows the development of experimental systems for non-amphimictic plant reconstruction is shown. The influence of in vitro growth conditions on haploid cells of unfertilized sugar beet ovules in the course of obtaining doubled haploid lines with high degree of homozygosity and maintenance of valuable breeding properties is considered. As compared to common inbreeding, this method shortens the time for development of homozygous material from 10–12 to 3–5 years, which is of great importance for speeding-up the breeding process. The results of studies on the culturing of mature sugar beet zygotic embryos based on in vitro selective systems have made it possible to improve the adaptive potential of plants and to provide complex resistance to environmental stress factors. Strict selection under abiotic stress conditions allowed creation of sugar beet isogenic lines with tolerance of drought, salinity, and soil acidity. It is shown that the proposed original design of mass-scale microclonal in vitro reproduction and deposition of elite plants as components of highly productive hybrids can be used to obtain seeds of uniform high-quality breeding material. The technologies developed by biotechnological methods are a topical and innovative direction of inquiry, since the application of these techniques to sugar beet breeding will promote obtaining of competitive hybrids with a set of commercially valuable traits. The combination of biotechnology methods, including tissue culture, and traditional breeding techniques is expected to provide an opportunity to obtain a new starting material to develop domestic varieties and hybrids of new generation with heterosis effect and a wide resistance spectrum persisting across generations.

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“…colchicine, trifluralin, etc.) that promote the antimitotic activity of the female gametophyte cells and increase the frequency of haploid regeneration [13]. The comparison between morphology of haploids and doubled haploid regenerants revealed the advantages of the first ones in the rate of shoot development and propagation in vitro.…”

Section: Introductionmentioning

confidence: 99%

SUGAR BEET (Beta vulgaris L.) HAPLOID PARTHENOGENESIS in vitro: FACTORS AND DIAGNOSTIC CHARACTERS

Zhuzhzhalova¹,

Podvigina²,

Znamenskaya³

et al. 2016

S-h. biol.

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A b s t r a c tTraditional obtaining of inbred lines and hybrids in sugar beet breeding requires a long time and is labour-consuming because of 2-year cycle of plant development, self-and cross-incompatibility, and inbreeding depression. To induce genotypic diversity in initial population, biotechnology methods including haploid parthenogenesis are promising. We have shown that, when inducing sugar beet (Beta vulgaris L.) haploids in vitro, express-diagnostics using phenotypic and embryological characters that are representative of periods of flowering shoots, organs and buds development, and stages of embryo sac, ovule and pollen grains formation is effective. The regenerative activity is observed in ovules of bud 1 to bud 25 located on ear part of pleochasium upward from the open flower. The nuclei and cells of female gametophyte of isolated ovules under in vitro conditions are capable of neoplasm at all stages of development, but the 8-nuclear or 7-celled embryo sacs are the most appropriate to morphogenesis and switching of development program from gametophyte to sporophyte type. Critical period of embryo sac development has been beforehand determined from the accompanying embryological characters -the presence of single-nuclear and two-three-celled pollen grains of anthers located in the same bud as ovules. The results we obtained indicate that hormonal composition of the Gamborg's B-5 (B5) medium is an important factor that effectively regulates direction of morphogenetic development in isolated ovules through direct regeneration (embryoidogenesis) or via callus (hemorhizogenesis) that is the evidence of totipotency of both sexual and somatic cells in the explant. The obtained data on in vitro reproduction of haploid regenerants add to available scientific notion of morphogenetic potential specificity in sugar beet plants. Stabilizing selection used to produce double haploids promotes detection of valuable morphological features of the regenerants. Determination of chromosome and chloroplast numbers in stomata guard cells as well as isozyme electrophoretic mobility (for Adh-1, Mdh-1, Mdh-2, Me-1, Idh-1, Idh-2, Gdh-1 loci) can serve as markers when inducing haploidy and producing homozygous restitution lines of sugar beet. Efficiency of RFLP-analysis method using Hind III restrictase that has allowed for the first time to identify haploid microclones according cytoplasm type is shown. Molecular markers have indicated that regenerants with normal cytoplasm (N) have one PCR-product of 800 bp in length not digested by Hind III. Two fragments (320 bp and 480 bp) of 800 bp product digestion are found in cytoplasmic male sterile (CMS) forms (S) that reflects combination of recessive and dominant genes. Obtaining haploid regenerants with sterile cytoplasm from initial population is of great theoretical and practical importance for sugar beet breeding thus facilitating the problem of producing homozygous lines with CMS and high-productive hybrids on the sterile basis.Keywords: sugar beet (Beta vulgaris L.) haploid ...

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Effects of Antimitotic Agents on Haploid Plant Production from Unpollinated Ovules of Sugar Beet (Beta VulgarisL.)

Abstract: ABSTRACT

Cited by 9 publications

References 16 publications

Kıyı Pancarı (Beta vulgaris subsp. maritima (L.) Arcang) Türünün In-vitro Şartlarda Mikro Çoğaltımı

Kıyı Pancarı (Beta vulgaris subsp. maritima (L.) Arcang) Türünün In-vitro Şartlarda Mikro Çoğaltımı

Biotechnological methods as a tool for efficient sugar beet breeding

SUGAR BEET (Beta vulgaris L.) HAPLOID PARTHENOGENESIS in vitro: FACTORS AND DIAGNOSTIC CHARACTERS

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