Effects of Antiviral Compounds on Symptoms and Infectivity of Cowpea Chlorotic Mottle Virus

Mancino, L. J. C.

doi:10.1094/pd-69-219

Cited by 4 publications

(2 citation statements)

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“…Chemical and heat therapy procedures have been evaluated for their ability to eradicate plant viruses (3,4,15,32). Even though many studies have been performed under field and greenhouse conditions, few chemicals have been found that will eliminate or substantially reduce the multiplication of viruses in infected plants (7,9,12,13,14,15,19,30). Heat treatment has also been examined as a method for eliminating viruses from plant tissue, and has historically been performed using potted plants grown under greenhouse conditions and (or) growth chamber conditions (21,32).…”

Section: Introductionmentioning

confidence: 99%

Effect of chemical and heat therapy on virus concentrations in in vitro potato plantlets

Griffiths¹,

Slack²,

Dodds³

1990

Can. J. Bot.

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Effect of chemical and heat therapy on virus concentrations in in vitro potato plantlets. Can. J. Bot. 68: 1515-1521.Potato (Solanurn tuberosum) plantlets were established in vitro to evaluate the effectiveness of chemicals and heat therapy for antiviral activity. Ribavirin was shown to be the most active chemical. Further studies were performed in which plantlets established from nodal cuttings were exposed to an alternating 4-h cycle of 35-31°C and ribavirin (20 mg/L) therapy for 4 weeks, and then tested quantitatively for virus titer by the enzyme-linked immunosorbent assay. Plantlets were further propagated via nodal cuttings at room temperature without exposure to ribavirin. Virus assays were performed and virus-free plantlets were grown out as mature plants for a final virus assay. Ribavirin alone or in combination with heat therapy was effective in reducing potato virus M, S, and X titers (between 10-and 60-fold). Virus-free plants were obtained from both treatments. For potato viruses Y and leafroll, about a fourfold reduction in titer was observed after the ribavirin-heat treatments. When multiple viruses were present, quantitative (10-to 20-fold) reductions in potato viruses M, S, X, and leafroll were observed and resulted in plants free of potato viruses M, S, and X. This protocol enables the investigator to quantitatively monitor the impact of a single variable on the virus-host interaction. We have alsoTeen able to demonstrate that virus elimination can be obtained without a requisite meristem-tip excision step. GRIFFITHS, H. M., SLACK, S. A . , et DODDS, J. H. 1990. Effect of chemical and heat therapy on virus concentrations in in vitro potato plantlets. Can. J. Bot. 68 : 1515-1521. Des plantules de Pomme de terre (Solanurn tuberosurn) furent Ctablies in vitro afin d'Cvaluer l'efficacitt de certains produits chimiques et de la thermothtrapie sur 1'activitC antivirale. La ribavirine a Ct C la substance chimique la plus active. D'autres Ctudes furent rCalisCes oh des plantules provenant de sections nodales furent exposCes i une alternance de cycles thkrapeutiques de 35-31°C d'une durCe de 4 h en presence de ribavirine (20 mg/L), pendant 4 semaines, et puis testtes quantitativement pour la concentration en virus par l'essai immunosorbant d'enzymes liCes. Les plantules furent de nouveau propagees via des sections nodales i temperature de la pikce et en l'absence de ribavirine. Des essais viraux furent rCalists et des plantules sans virus furent cultivtes jusqu'au stade de plantes matures en vue d'un essai viral final. La ribavirine seule ou en combinaison avec la themotherapie a rCduit la concentration des virus de la pomme de terre M, S et X de 10 i 60 fois. Des plantes sans virus furent obtenues des deux traitements. Quant au virus Y et i celui de I'enroulement, l'on a observC une rkduction quadruple de la concentration pour les traitements ribavirine-chaleur. Lorsque plusieurs virus Ctaient prksents, des reductions quantitatives des virus M, S, X et de l'enroulement (rtductions de 10 i 20 fois) furen...

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Section: Introductionmentioning

confidence: 99%

Effect of chemical and heat therapy on virus concentrations in in vitro potato plantlets

Griffiths¹,

Slack²,

Dodds³

1990

Can. J. Bot.

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“…Amantadine, when sprayed on cowpea exhibiting symptoms of Cowpea chlorotic mottle virus (Mancino and Agrios 1984). 5-bromouracil (RNA synthesis inhibitor) has been tested against Tobacco mosaic virus that produced local lesions on Phaseolus vulgaris (Dhaliwal 1975).…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Indexing and production of virus-free chrysanthemums

Ram¹,

Verma²,

Singh³

et al. 2005

Biologia plant.

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Chrysanthemum morifolium cv. Regol Time plants were found to be infected with Chrysanthemum B carlavirus (CVB). They were made CVB-free by using meristem tip culture, chemotherapy and thermotherapy. The plants were indexed by biological assay, double antibody sandwitch enzyme linked immunosorbent assay (DAS-ELISA) and reverse transcriptase polymerase chain reaction (RT-PCR). The maximum number of virus-free plants (26.7 %, as indexed by RT-PCR) was obtained with 2-thiouracil at 0.04 g dm -3 concentration. Only 12 % plants were found to be virus-free, after being kept at 38 °C for 30 d. For indexing CVB in chrysanthemums, RT-PCR was found to be the most reliable method.Additional key words: antiviral compounds, Chrysanthemum B carlavirus, Chrysanthemum morifolium, DAS-ELISA, in vitro production, RT-PCR.

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Ribavirin targets sugar transporter 6 to suppress acquisition and transmission of rice stripe tenuivirus by its vector Laodelphax striatellus

Hajano

Raza

Zhang

et al. 2020

Pest Management Science

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BACKGROUND: Rice stripe tenuivirus (RSV) is one of the most destructive pathogens of rice and other cereal crops. The virus is transmitted by the small brown planthopper (SBPH, Laodelphax striatellus) in a circulative-propagative manner. Thus, blocking transmission by the insect vector would provide an effective strategy to prevent epidemic outbreaks of the disease. RESULTS: In this study, we explored the effect of ribavirin on acquisition and transmission of the virus by specifically inhibiting the expression of sugar transporter 6 (LsSt-6), which was recently reported as a key vector component for RSV transmission. Ribavirin at the highest concentration tested (250 ∼mol L-1) significantly reduced RSV acquisition and transmission efficiency by SBPHs through inhibiting LsSt-6 messenger RNA (mRNA) level. Survival of the model insect Spodoptera frugiperda cell line (Sf9) was 95.0 ± 2.2 and 85.6 ± 2.1% after exposure to 250 ∼mol L-1 ribavirin or 8-azaguanine, respectively. Further study confirmed that 250 ∼mol L-1 ribavirin also significantly reduced LsSt-6 mRNA and protein levels in Sf9 cells. However, 8-azaguanine did not significantly inhibit viral infectivity and LsSt-6 mRNA levels in SBPH or the Sf9 cell line. CONCLUSION: This result provides evidence that ribavirin has the potential to disrupt LsSt-6 expression but not others like viral RNAs to prevent acquiring RSV, which leads to less viral accumulation in SBPH tissues and thereby lower transmission efficiency.

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Effects of Antiviral Compounds on Symptoms and Infectivity of Cowpea Chlorotic Mottle Virus

Cited by 4 publications

References 0 publications

Effect of chemical and heat therapy on virus concentrations in in vitro potato plantlets

Effect of chemical and heat therapy on virus concentrations in in vitro potato plantlets

Indexing and production of virus-free chrysanthemums

Ribavirin targets sugar transporter 6 to suppress acquisition and transmission of rice stripe tenuivirus by its vector Laodelphax striatellus

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