Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen

Wang, Hongtao; Lu, Pingli; Yuan, Chongshan; Zhao, Jing; Liu, Hongyu; Lu, Wenfa; Wang, Jun

doi:10.3390/ani11061506

Cited by 9 publications

(7 citation statements)

References 34 publications

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“…The authors noted that API was exceptionally effective for the sperm to acquire better mitochondrial activity, confirming the ability of API to trespass relatively freely through the sperm cell, and thus exhibit its activities intracellularly and have a better protective effect on the mitochondrial membrane. What is more, Wang et al [ 255 ] confirmed that API could reduce bacterial contamination and improve the kinematic parameters and abnormal semen movement in frozen semen as a consequence of bacteriospermia.…”

Section: Apigeninmentioning

confidence: 99%

The Role of Selected Natural Biomolecules in Sperm Production and Functionality

et al. 2021

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Emerging evidence from in vivo as well as in vitro studies indicates that natural biomolecules may play important roles in the prevention or management of a wide array of chronic diseases. Furthermore, the use of natural compounds in the treatment of male sub- or infertility has been proposed as a potential alternative to conventional therapeutic options. As such, we aimed to evaluate the effects of selected natural biomolecules on the sperm production, structural integrity, and functional activity. At the same time, we reviewed their possible beneficial or adverse effects on male reproductive health. Using relevant keywords, a literature search was performed to collect currently available information regarding molecular mechanisms by which selected natural biomolecules exhibit their biological effects in the context of male reproductive dysfunction. Evidence gathered from clinical trials, in vitro experiments and in vivo studies suggest that the selected natural compounds affect key targets related to sperm mitochondrial metabolism and motion behavior, oxidative stress, inflammation, DNA integrity and cell death. The majority of reports emphasize on ameliorative, stimulating and protective effects of natural biomolecules on the sperm function. Nevertheless, possible adverse and toxic behavior of natural compounds has been indicated as well, pointing out to a possible dose-dependent impact of natural biomolecules on the sperm survival and functionality. As such, further research leading to a deeper understanding of the beneficial or adverse roles of natural compounds is necessary before these can be employed for the management of male reproductive dysfunction.

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Section: Apigeninmentioning

confidence: 99%

The Role of Selected Natural Biomolecules in Sperm Production and Functionality

et al. 2021

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“…Semen samples were collected using an artificial vagina twice weekly per bull, in which the cryopreservation and sperm quality assessment were conducted in accordance with the standard commercial procedure (Wang et al, 2021). The criteria for cryopreservation were as follows: semen samples were sent to the laboratory within 30 min at a concentration of at least 1 × 10 9 spermatozoa/ ml, with a sperm motility ≥70% and an abnormality level ≤15%.…”

Section: Bull Semen Collectionmentioning

confidence: 99%

Epigallocatechin 3‐gallate improves the quality of bull semen cryopreservation

Wang

Yuan

et al. 2021

Andrologia

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To determine the effects of epigallocatechin-3-gallate (EGCG) on the cryopreservation of bovine semen, epigallocatechin-3-gallate dissolved with double distilled water to 0.2, 0.4, 0.6 and 0.8 mg/ml were added to the cryopreservation diluent of the bull semen. Then, we used computer-assisted analysis of semen kinematic parameters, staining method to detect membrane function, acrosome integrity, enzyme-linked immunosorbent assay to detect catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehydes (MDA) and reactive oxygen levels.The results showed that adding 0.6 mg/L of epigallocatechin-3-gallate could improve the cryopreserved sperm quality, which significantly increased the total motility, distance average path, distance straight line, distance curved line, average path velocity, curvilinear-velocity, straight-line velocity, amplitude of lateral head displacement and beat/cross frequency, as well as sperm CAT, GSH-Px and SOD levels (p < 0.05), whilst reducing the reactive oxygen species and MDA levels (p < 0.05). Hence, these results indicate that the addition of 0.6 mg/ml of EGCG has a protective effect on the cryopreservation of the bovine semen.

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“…For example, a study on bull semen found that adding 0.2 mmol/L of apigenin to the freezing solution improved the sperm ability to move, maintain their shape, and penetrate of the egg. Apigenin also increased the antioxidant activity of the sperm, which helped them resist against oxidative damage caused by freezing and thawing 17 . However, it is important to note that the concentration of apigenin used can have different effects.…”

Section: Introductionmentioning

confidence: 99%

“…It has been studied for its possible benefits in preserving sperm quality during freezing and thawing processes. Some studies have shown that apigenin can improve the survival and function of sperm cells after cryopreservation, especially in bulls and boars 17 , 18 . For example, a study on bull semen found that adding 0.2 mmol/L of apigenin to the freezing solution improved the sperm ability to move, maintain their shape, and penetrate of the egg.…”

Section: Introductionmentioning

confidence: 99%

“…There are many fruitful scientific areas and practical uses to explore if apigenin works well in this new bird model. While apigenin has shown great promise in protecting sperm from freeze–thaw injuries in bulls and boars 17 , 18 , its effects on avian semen cryopreservation remain unexplored. The aim of this study was to investigate the optimal dose of apigenin for enhancing the cryosurvival of rooster sperm after freezing and thawing.…”

Section: Introductionmentioning

confidence: 99%

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Apigenin supplementation substantially improves rooster sperm freezability and post-thaw function

Najafi,

Mohammadi,

Sharifi

et al. 2024

Sci Rep

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This pioneering research investigated apigenin potential to augment rooster sperm cryosurvival in an extender model. Apigenin is a natural antioxidant flavonoid showing promise for improved post-thaw sperm function. However, its effects on avian semen cryopreservation remain unexplored. This first study supplemented rooster sperm Lake extender with 0, 50, 100, 200, 400 μmol/L apigenin to determine the optimal concentrations for post-thaw quality. Supplementation with 100 μmol/L apigenin resulted in significant enhancements in total motility (from 41.5% up to 71.5%), progressive motility (18.1% to 29.1%) (p < 0.05), membrane integrity (40% to 68%), mitochondrial function (p < 0.001), viability (37% to 62%) and total antioxidant capacity (p < 0.001) compared to the control. It also substantially reduced percentages of abnormal morphology, reactive oxygen species and apoptosis (p < 0.001). Although 200 μmol/L apigenin significantly enhanced some attributes, effects were markedly lower than 100 μmol/L. Higher doses did not improve cryoprotective parameters. This indicates 100 μmol/L as the optimal apigenin concentration. This represents the first report of apigenin protecting rooster sperm from cryodamage. The natural antioxidant improved post-thaw sperm quality, likely by suppressing oxidative stress and apoptosis. Apigenin shows promise for enhancing rooster sperm cryosurvival.

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Effects of Apigenin and Astragalus Polysaccharide on the Cryopreservation of Bull Semen

Cited by 9 publications

References 34 publications

The Role of Selected Natural Biomolecules in Sperm Production and Functionality

The Role of Selected Natural Biomolecules in Sperm Production and Functionality

Epigallocatechin 3‐gallate improves the quality of bull semen cryopreservation

Apigenin supplementation substantially improves rooster sperm freezability and post-thaw function

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