Chlorine, sodium and silicon have long been considered as elements that are possibly essential to the growth and development of sugar beets and other plants. Of these elements Cl has been found just recently by Broyer et al (2) to correct a severe nutritional deficiency of the tomato plant when grown in low halide culture solutions. Raleigh (28) Much has been written about Na in terms of direct and indirect effects upon plant growth (5,9,10,19,20,23 In view of these reports, often indefinite in their conclusions, it was thought worthwhile to re-examine Na, Si and Cl in relation to the growth of sugar beet plants by adding these elements to otherwise complete culture solutions prepared from "chemically pure" salts. The initial experiment was of a factorial design using two levels of the elements in question. The first level of Cl, Na or Si was the amount present as an impurity in the salts and distilled water. The other level was an exact quantity added. It was thought that if the requirement for any one or more of these elements was larger than that provided by the impurities present, it would be revealed by the increased growth of the plants at the higher nutrient level. If increased growth was not obtained, the second step to be taken would include a further purification of all salts for each of the elements not found to enhance growth or development of the sugar beet plant. Two other factors were also included in the experiment; K at 2 levels and 2 beet varieties, giving a total of 5 factors each at 2 levels.
METHODS AND MATERIALSPLANT CULTURE: The sugar beet (Beta vulgaris) varieties used in the 25 factorial experiment were U.S. 22/3 and GW 304, and for the chloride series experiment, U.S. 75