Effects of arachidonic acid metabolites and other compounds on the CTLL assay for interleukin-2

Eskandari, Mark K.; Kunkel, Steven L.; Remick, Daniel G.

doi:10.1016/0022-1759(89)90057-4

Cited by 7 publications

(2 citation statements)

References 13 publications

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“…Similarly, U-937 cells which secrete Interleukin-1 (IL-1) constitutively were treated with Perna and the supernatants added to the sensitive cell line A375.S2 [ 33 , 34 ]. Likewise, Jurkat E6-1 and EL-4 mouse thymoma cells which when stimulated with Ionomycin and phorbol myristate acetate (PMA) respectively secrete IL-2, were treated with Perna and the supernatants were added to the responder cell line CTLL-2 [ 35 - 38 ]. Finally, LS174T colon adenocarcinoma cells that constitutively secrete IL-6 were treated with the Perna extracts and the supernatants added to the responder 7TD1 cell line [ 39 - 41 ].…”

Section: Methodsmentioning

confidence: 99%

In vitro modulation of inflammatory cytokine and IgG levels by extracts of Perna canaliculus

Mani

Lawson

2006

BMC Complement Altern Med

114

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BackgroundInflammation is a predominant characteristic of autoimmune diseases which is characterized by the increased expression of pro-inflammatory cytokines. Soon to be published work from our laboratory has shown that ingestion of Perna canaliculus prevents the development of autoimmune diseases such as Systemic Lupus Erythematosus and rheumatoid arthritis in laboratory animals. The current paper attempts to illustrate how Perna can alleviate inflammation by modulating inflammatory cytokines, cyclooxygenase enzymes and Immunoglobulin-G (IgG) levels.MethodsIn the present study, hydrochloric acid [HCl] and Tween-20 were used to develop extracts of Perna. These extracts were assayed for protein content. Increasing concentrations of these extracts were then tested in cell culture for modulation of inflammatory cytokine, cyclooxygenase enzymes and IgG levels. Parallel tests were run using an available glycogen extract of Perna as a comparison to our in-house laboratory preparations.ResultsTween-20 Perna extracts were found to be more stable and less toxic in cell culture than HCl digest of Perna. They also assayed higher in protein content that HCl extracts. Although both extracts inhibited IgG production in V2E9 hybridomas, Tween-20 extracts were more consistent in IgG suppression than HCl extracts. Overall Tween-20 extracts effectively decreased levels of TNF-α, IL-1, IL-2 and IL-6 as observed using cytokine bioassays. Twenty micrograms of Tween-20 Perna extracts induced such significant decreases in inflammatory cytokine production that when tested on sensitive cell lines, they very nearly abolished the decrease in viability induced by these cytokines. Tween-20 extracts effectively inhibited both COX-1 and COX-2 cyclooxygenase activity. As a comparison, the glycogen extract also demonstrated a similar though weaker effect on COX-1 and COX-2 enzymes. The active components of both extracts (Tween-20 and glycogen) were observed to possess molecular weights above 100 kDa. Although the anti-cytokine activity of the Tween-20 extract was destroyed by Proteinase-K treatment, the anti-COX-1 and anti-COX-2 activity of both the extracts were not sensitive to protease treatment.ConclusionWe have successfully demonstrated modulation in the levels of inflammatory cytokines, cyclooxygenase enzymes and immunoglobulins by our in-house laboratory preparations of Perna canaliculus, whereby suggesting an immunomodulatory role of Perna canaliculus in regulating inflammation.

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Section: Methodsmentioning

confidence: 99%

In vitro modulation of inflammatory cytokine and IgG levels by extracts of Perna canaliculus

Mani

Lawson

2006

BMC Complement Altern Med

114

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show abstract

“…We choose to apply dexamethasone to suppress the tissue responses since dexamethasone is a glucocorticosteroid with a broad suppressing effect on inflammatory agents such as IL-1-Beta, TNF-alpha, and prostaglandines [23][24][25][26]. One may argue, that dexamethasone is not applicable for all encapsulated cell approaches since dexamethasone has a reported effect on insulin resistance [27][28][29] and can consequently therefore not be applied in transplantation of encapsulated pancreatic islets.…”

Section: Article In Pressmentioning

confidence: 99%

Deletion of the tissue response against alginate-pll capsules by temporary release of co-encapsulated steroids

et al. 2005

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Interleukin-1α and interleukin-2 in cerebrospinal fluid of schizophrenic subjects

El‐Mallakh

Suddath

Wyatt

1993

Progress in Neuro-Psychopharmacology and Biological Psychiatry

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Effects of arachidonic acid metabolites and other compounds on the CTLL assay for interleukin-2

Cited by 7 publications

References 13 publications

In vitro modulation of inflammatory cytokine and IgG levels by extracts of Perna canaliculus

In vitro modulation of inflammatory cytokine and IgG levels by extracts of Perna canaliculus

Deletion of the tissue response against alginate-pll capsules by temporary release of co-encapsulated steroids

Interleukin-1α and interleukin-2 in cerebrospinal fluid of schizophrenic subjects

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