Effects of Aroclor 1254 on LH and 17,20β-P secretion in female Prussian carp (Carassius gibelio Bloch) in the spawning season

Abstract

ABSTRACT:The effects of Aroclor 1254 on the secretion of luteinizing hormone (LH) and 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P) in female Prussian carp (Carassius gibelio Bloch) at the time of their natural spawning were determined. Aroclor 1254 at doses of 0.01, 0.1, and 1 mg/kg body weight was dissolved in 500 ml of oil and was administered three times intraperitoneally or rectally to female Prussian carp every 24 h for three days. Blood samples were collected after 3 days of Aroclor 1254 administration to determine the LH and 17,20β-P concentrations using ELISA. At 6, 12, and 24 h after injection of gonadotropin-releasing hormone analogue (GnRH-A) blood samples were collected for stimulated LH secretion determination. Aroclor 1254 administered intraperitoneally (0.1 and 1 mg/kg) and rectally (0.01 and 1 mg/kg) significantly increased spontaneous LH secretion. In the case of GnRH-A-stimulated LH release, Aroclor 1254 (administered intraperitoneally only) at concentrations of 0.1 and 1 mg/kg significantly decreased gonadotropin release. The intraperitoneal injections of the lowest tested concentration of Aroclor 1254 also significantly decreased 17,20β-P secretion. The results show that Aroclor 1254 can affect the reproductive system of Prussian carp by changing the secretion of two very important hormones, LH and 17,20β-P, at the time of natural spawning.

Distribution and estrogenic potential of endocrine disrupting chemicals (EDCs) in estuarine sediments from Mumbai, India

“…Natural and synthetic estrogens can induce endocrine disrupting effects in the aquatic organisms, in both male and female hormonal system (Hatef et al 2012;Socha et al, 2013), or even stimulation of feminization or hermaphroditism in fish at very low concentrations (MacLatchy and Van Der Kraak 1995; Thorpe et al 2003;Nash et al 2004). As limitations associated with bacteria application as pollution tracers, human and animal sterols are currently being used as indicators of anthropogenic contamination in environmental compartments.…”

“…To know the fate of EDCs in sediments is critical for their environmental exposure and risk assessment (Peck et al 2004;Karnjanapiboonwong et al 2010). Socha et al (2013) demonstrate that the short-term exposure to Aroclor 1254 in the spawning season might impair reproduction in female Carassius gibelio by affecting the endocrine system by altering the secretion of luteinizing hormone (LH) and 17,20β-dihydroxy-4-pregnen-3-one (17,20β-P).…”

Sex Dependent Action of Aroclor 1254 on Basal and sGnRHa-Stimulated Secretion of LH from the Pituitary Cells of Common Carp, Cyprinus carpio L.

“…like polychlorinated biphenyls, present in an increasing amount in a polluted environment, may accumulate in organisms living in water (Van Geest et al, 2011). They cause reproductive dysfunctions in vertebrates (Gregoraszczuk et al, 2005;Andric et al, 2006) and in different fish species they cause permanent structural damage to the gonads (Garcia et al, 2016) or affect the secretion of endogenous hormones regulating reproduction (Khan and Thomas, 1997Thomas and Doughty, 2004;Benninghoff and Thomas, 2005;Socha et al, 2013a). The site of their action probably includes all the glands on the hypothalamo-pituitary-gonadal axis.…”