Effects of Aroclor 1254 on femoral bone metabolism in adult male Wistar rats

Govindan, Ramajayam; Sridhar, M.; Karthikeyan, S.; Lavanya, R; Veni, S.; Vignesh, R.; Ilangovan, R.; Djody, S. Sitta; Gopalakrishnan, V. K.; Arunakaran, J.

doi:10.1016/j.tox.2007.08.086

Cited by 34 publications

(21 citation statements)

References 49 publications

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“…An increase of the ␤ subunit could thus promote the formation of the active enzyme, essentially favoring collagen biosynthesis (83). However, this hypothesis is in contradiction with previous studies highlighting a reduction of collagen synthesis in the presence of PCBs (84,85). Because a protein-disulfide isomerase activity has also been assumed for prolyl 4-hydroxylase ␤ (86, 87), the overexpression of the P4hb subunit is in agreement with the up-regulation of GPR58 and reinforces the hypothesis that tadpole cells and tissues exposed to PCBs initiate defensive responses for protection against modified and misfolded proteins.…”

Section: Discussionmentioning

confidence: 83%

Protein Expression Profiling in the African Clawed Frog Xenopus laevis Tadpoles Exposed to the Polychlorinated Biphenyl Mixture Aroclor 1254

Gillardin

Silvestre

Dieu

et al. 2009

Molecular & Cellular Proteomics

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Exposure to environmental pollutants such as polychlorinated biphenyls (PCBs) is now taken into account to partly explain the worldwide decline of amphibians. PCBs induce deleterious effects on developing amphibians including deformities and delays in metamorphosis. However, the molecular mechanisms by which they express their toxicity during the development of tadpoles are still largely unknown. A proteomics analysis was performed on developing Xenopus laevis tadpoles exposed from 2 to 5 days postfertilization to either 0.1 or 1 ppm Aroclor 1254, a PCB mixture. Two-dimensional DIGE with a minimal labeling method coupled to nanoflow liquid chromatographytandem mass spectrometry was used to detect and identify proteins differentially expressed under PCBs conditions. Results showed that 59 spots from the 0.1 ppm Aroclor 1254 condition and 57 spots from the 1 ppm Aroclor 1254 condition displayed a significant increase or decrease of abundance compared with the control. In total, 28 proteins were identified. The results suggest that PCBs induce mechanisms against oxidative stress (peroxiredoxins 1 and 2), adaptative changes in the energetic metabolism (enolase 1, glycerol-3-phosphate dehydrogenase, and creatine kinase muscle and brain types), and the implication of the unfolded protein response system (glucose-regulated protein, 58 kDa). They also affect, at least at the highest concentration tested, the synthesis of proteins involved in normal cytogenesis (␣-tropomyosin, myosin heavy chain, and ␣-actin). For the first time, proteins such as aldehyde dehydrogenase 7A1, CArG binding factor-A, prolyl 4-hydroxylase ␤, and nuclear matrix protein 200 were also shown to be up-regulated by PCBs in developing amphibians. These data argue that protein expression reorganization should be taken into account while estimating the toxicological hazard of wild amphibian populations exposed to PCBs. Molecular & Cellular Proteomics 8:596 -611, 2009.

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Section: Discussionmentioning

confidence: 83%

Protein Expression Profiling in the African Clawed Frog Xenopus laevis Tadpoles Exposed to the Polychlorinated Biphenyl Mixture Aroclor 1254

Gillardin

Silvestre

Dieu

et al. 2009

Molecular & Cellular Proteomics

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“…The left femur was grinded and about 100 mg was taken and homogenized with 2 ml of 0.1 M Tris-HCl buffer (pH 7.2). The homogenate was centrifuged at 3000 rpm for 30 min at 4°C and the supernatant was used for biochemical and oxidative stress marker estimations (Ramajayam et al, 2007). All animal procedures were conducted according to the "Guide for the Care and Use of Laboratory Animals" and were approved by the local Cairo University animal research ethics committee.…”

Section: Experimental Designmentioning

confidence: 99%

Antiosteoporotic effect of Coelatura aegyptiaca shell powder on ovariectomized rats

Sayed¹,

Soliman²,

Fahmy³

et al. 2013

Afr. J. Pharm. Pharmacol.

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The efficacy of Coelatura aegyptiaca shell powder (CES, 500 mg/kg BW) was evaluated as a calcium supplement in ovariectomized (OVX) rats for ten weeks of treatment. The biochemical components and the antioxidant properties of the shell powder were determined. The bone mineral density (BMD), bone mineral content (BMC), calcium (Ca) and phosphorus (P) contents in serum and bone, serum total alkaline phosphatase (TALP) and bone alkaline phosphatase (BALP), serum calcitonin and parathyroid (PTH) hormones were determined. Furthermore, some of the oxidative stress markers [malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx) and total antioxidant capacity (TAC)] were estimated in bone. The current study revealed that CES contained 19.38% Ca, 0.315% P as well as some of antioxidant amino acids which have a potent antioxidant activity against 1,1-diphenylpicrylhydrazyl (DPPH) free radical. Administration of CES to OVX rats increased BMD, BMC, tibial Ca and P contents and BALP activity, as compared to OVX rats. An ameliorative effect was recorded in the levels of calcitonin, PTH, MDA, SOD, GPx and TAC subsequent to CES administration to OVX rats.This ameliorative effect of CES powder against osteoporosis may be attributed to its antioxidant efficacy and/or to its Ca content. In conclusion, CES may have the potential to develop a clinically useful anti-osteoporotic agent, since its effect was comparable with alendronate (6.5 mg/kg BW/week).

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“…This effect indicates that chromium administration induced stress in the rat subjects, and that the ascorbic acid stored in their femurs was rapidly oxidized [13]. Beyond the aforementioned, it also known that antioxidant deficiency has a negative impact on bone mass [27].…”

Section: Discussionmentioning

confidence: 95%

The peripheral quantitative computed tomographic and densitometric analysis of skeletal tissue in male Wistar rats after chromium sulfate treatment

Bieńko¹,

Radzki²,

Wolski³

2017

Ann Agric Environ Med.

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Introduction. This study evaluates the effects of three different doses of chromium sulphate on bone density and the tomographic parameters of skeletal tissue of rats. Materials and method. The experiment was performed on 40 male Wistar rats which received, by gavage, during 90 days, a chromium sulphate in either a daily dose of 400, 600 or 800 µg/kg BW. At the end of experiment, the rats were scanned using the densitometry method (DXA) to determine the bone mineral density, bone mineral content of total skeleton and vertebral column (L2-L4) and parameters of body composition (Lean Mass and Fat Mass). The isolated femora were scanned using peripheral a quantitative computed tomography method (pQCT) for a separate analysis of the trabecular and cortical bone tissue. The ultimate strength, work to ultimate and the Young modulus of femora was also investigated by the threepoint bending test. Results. The negative impact of chromium was observed in relation to bone tissue. All doses significantly decreased total skeleton density and mineral content, and also had impact upon the isolated femora and vertebral column. Trabecular volumetric bone mineral density and trabecular bone mineral content measured by pQCT in distal femur metaphysis were significantly lower in the experimental groups than in the control. Higher doses of chromium also significantly decreased values of ultimate strength and Young modulus in the investigated femora. Conclusions. The results of the experiment demonstrate that chromium sulphate is dose dependent, and exerts a disadvantageous effect on the skeleton, as it decreases bone density and resistance.

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Effects of Aroclor 1254 on femoral bone metabolism in adult male Wistar rats

Cited by 34 publications

References 49 publications

Protein Expression Profiling in the African Clawed Frog Xenopus laevis Tadpoles Exposed to the Polychlorinated Biphenyl Mixture Aroclor 1254

Protein Expression Profiling in the African Clawed Frog Xenopus laevis Tadpoles Exposed to the Polychlorinated Biphenyl Mixture Aroclor 1254

Antiosteoporotic effect of Coelatura aegyptiaca shell powder on ovariectomized rats

The peripheral quantitative computed tomographic and densitometric analysis of skeletal tissue in male Wistar rats after chromium sulfate treatment

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