Effects of Arsenic and Iron on the Community and Abundance of Arsenite-Oxidizing Bacteria in an Arsenic-Affected Groundwater Aquifer

Pipattanajaroenkul, Phurinat; Chotpantarat, Srilert; Termsaithong, Teerasit; Sonthiphand, Prinpida

doi:10.1007/s00284-021-02418-8

Cited by 12 publications

(4 citation statements)

References 45 publications

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“…However, the IIRP amendment increased the potential for As(III) oxidation in the anaerobic zone, as suggested by the increase in aioA gene abundance in IIRP-TWs in the bottom layer. The observation is consistent with Pipattanajaroenkul et al, who found that the abundances of As(III) oxidizers and aioA genes were positively correlated with Fe content in an anaerobic groundwater aquifer. Fe(III) reduction at the bottom layer of IIRP-TWs would increase the bioavailability of adsorbed As(III), favoring the growth of As(III) oxidizers and thus promoting As(III) oxidation.…”

Section: Discussionmentioning

confidence: 96%

Immobile Iron-Rich Particles Promote Arsenic Retention and Regulate Arsenic Biotransformation in Treatment Wetlands

Liu

Häggblom

et al. 2022

Environ. Sci. Technol.

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Remediation of arsenic (As)-contaminated wastewater by treatment wetlands (TWs) remains a technological challenge due to the low As adsorption capacity of wetland substrates and the release of adsorbed As to pore water. This study investigated the feasibility of using immobile iron-rich particles (IIRP) to promote As retention and to regulate As biotransformation in TWs. Iron-rich particles prepared were immobilized in the interspace of a gravel substrate. TWs with IIRP amendment (IIRP-TWs) achieved a stable As removal efficiency of 63 ± 4% over 300 days, while no As removal or release was observed in TWs without IIRP after 180 days of continuous operation. IIRP amendment provided additional adsorption sites and increased the stability of adsorbed As due to the strong binding affinity between As and Fe oxides. Microbially mediated As(III) oxidation was intensified by iron-rich particles in the anaerobic bottom layer of IIRP-TWs. Myxococcus and Fimbriimonadaceae were identified as As(III) oxidizers. Further, metagenomic binning suggested that these two bacterial taxa may have the capability for anaerobic As(III) oxidation. Overall, this study demonstrated that abiotic and biotic effects of IIRP contribute to As retention in TWs and provided insights into the role of IIRP for the remediation of As contamination.

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Section: Discussionmentioning

confidence: 96%

Immobile Iron-Rich Particles Promote Arsenic Retention and Regulate Arsenic Biotransformation in Treatment Wetlands

Liu

Häggblom

et al. 2022

Environ. Sci. Technol.

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“…Traditional molecular fingerprint methods, such as T-RFLP, DGGE, and clone library, have been used to investigate the distribution and diversity of aioA genes Han et al, 2017;Pipattanajaroenkul et al, 2021). These studies have reported that the community of As(III)-oxidizing bacteria in paddy soil was dominated by Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria (Dong et al, 2014;Jia et al, 2014), most of which are affiliated with Rhizobiales, Burkholderiales, Comamonadaceae, Phyllobacteriaceae, Bradyrhizobiaceae, and Methylobacteriaceae (Zhang S. Y. et al, 2015).…”

Section: Differences In the Aioa Genes From The Paddy Soil And Sediment Samplesmentioning

confidence: 99%

“…In arsenic contaminated soils, phosphate-extractable As was one of the most important factors in shaping the As transformation functional genes (Gu et al, 2017). Furthermore, As(III) was identified as the major environmental factor influencing the community and abundance of As(III)-oxidizing bacteria in groundwater (Pipattanajaroenkul et al, 2021). Normally, As fractionation in soil and sediment includes dissolved, poorly crystalline Fe (oxyhydr)oxide-bound (extracted by NH 4 + -oxalate) and Fe (oxyhydr)oxides-bound (extracted by KH 2 PO 4 and ascorbic acid) forms that represent the different degrees of bioavailability (Liu et al, 2015).…”

Section: Environmental Factors Driving the Diversity Of As(iii)-oxidizing Bacteria In Natural Environmentsmentioning

confidence: 99%

New Arsenite Oxidase Gene (aioA) PCR Primers for Assessing Arsenite-Oxidizer Diversity in the Environment Using High-Throughput Sequencing

Qiao

et al. 2021

Front. Microbiol.

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Gene encoding the large subunit of As(III) oxidase (AioA), an important component of the microbial As(III) oxidation system, is a widely used biomarker to characterize As(III)-oxidizing communities in the environment. However, many studies were restricted to a few sequences generated by clone libraries and Sanger sequencing, which may have underestimated the diversity of As(III)-oxidizers in natural environments. In this study, we designed a primer pair, 1109F (5′-ATC TGG GGB AAY RAC AAY TA−3′) and 1548R (5′-TTC ATB GAS GTS AGR TTC AT−3′), targeting gene sequence encoding for the conserved molybdopterin center of the AioA protein, yielding amplicons approximately 450 bp in size that are feasible for highly parallel amplicon sequencing. By utilizing in silico analyses and the experimental construction of clone libraries using Sanger sequencing, the specificity and resolution of 1109F/1548R are approximated with two other previously published and commonly used primers, i.e., M1-2F/M3-2R and deg1F/deg1R. With the use of the 1109F/1548R primer pair, the taxonomic composition of the aioA genes was similar both according to the Sanger and next-generation sequencing (NGS) platforms. Furthermore, high-throughput amplicon sequencing using the primer pair, 1109F/1548R, successfully identified the well-known As(III)-oxidizers in paddy soils and sediments, and they also revealed the differences in the community structure and composition of As(III)-oxidizers in above two biotopes. The random forest analysis showed that the dissolved As(III) had the highest relative influence on the Chao1 index of the aioA genes. These observations demonstrate that the newly designed PCR primers enhanced the ability to detect the diversity of aioA-encoding microorganisms in environments using highly parallel short amplicon sequencing.

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“…Mobility of As in an aquifer is the main factor leading to its accumulation in water which is controlled by sediment geochemistry, evapotranspiration, flow-through conditions, pH, redox potential, microbial community, and ion availability (Mladenov et al, 2014; Pipattanajaroenkul et al, 2021). Because of the strong affinity of As and adsorption ability of Fe(III) (oxyhydr)oxides, the reductive dissolution of Fe(III) minerals plays an important role in As groundwater accumulation (Yang et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Nitrate leaching and its implication for Fe and As mobility in a Southeast Asian aquifer

Głodowska

Smith

et al. 2022

Preprint

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The drinking water quality of millions of people in South and Southeast Asia is at risk due to arsenic (As) contamination of groundwater and insufficient access to water treatment facilities. Intensive use of nitrogen (N) fertilizer increases the possibility of nitrate (NO3-) leaching into aquifers, yet very little is known about how the N cycle will interact with and affect the iron (Fe) and As mobility in aquifers. We hypothesized that input of NO3- into highly methanogenic aquifers can stimulate nitrate-dependent anaerobic methane oxidation (N-DAMO) and subsequently help to remove NO3- and decrease CH4 emission. We, therefore, investigated the effects of N input into aquifers and its effect on Fe and As mobility, by running a set of microcosm experiments using aquifer sediment from Van Phuc, Vietnam supplemented with 15NO3- and 13CH4. Additionally, we assessed the effect of N-DAMO by inoculating the sediment with two different N-DAMO enrichment cultures (N-DAMO(O) and N-DAMO(V)). We found that native microbial communities and both N-DAMO enrichments could efficiently consume nearly 5 mM NO3- in 5 days. In an uninoculated setup, NO3- was preferentially used over Fe(III) as an electron acceptor and consequently inhibited Fe(III) reduction and As mobilization. The addition of N-DAMO(O) and N-DAMO(V) enrichment cultures led to substantial Fe(III) reduction followed by the release of Fe2+ (0.190 mM and 0.350 mM, respectively) and buildup of sedimentary Fe(II) (11.20 mM and 10.91 mM, respectively) at the end of the experiment (day 64). Only in the N-DAMO(O) inoculated setup, As was mobilized (27.1 ug/L), while in the setup inoculated with N-DAMO(V) a significant amount of Mn (24.15 mg/L) was released to the water. Methane oxidation and 13CO2 formation were observed only in the inoculated setups, suggesting that the native microbial community did not have sufficient potential for N-DAMO. An increase of NH4+ implied that dissimilatory nitrate reduction to ammonium (DNRA) took place in both inoculated setups. The archaeal community in all treatments was dominated by Ca. Methanoperedens while the bacterial community consisted largely of various denitrifiers. Overall, our results suggest that input of N fertilizers to the aquifer decreases As mobility and that CH4 cannot serve as an electron donor for the native NO3- reducing community.

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Effects of Arsenic and Iron on the Community and Abundance of Arsenite-Oxidizing Bacteria in an Arsenic-Affected Groundwater Aquifer

Cited by 12 publications

References 45 publications

Immobile Iron-Rich Particles Promote Arsenic Retention and Regulate Arsenic Biotransformation in Treatment Wetlands

Immobile Iron-Rich Particles Promote Arsenic Retention and Regulate Arsenic Biotransformation in Treatment Wetlands

New Arsenite Oxidase Gene (aioA) PCR Primers for Assessing Arsenite-Oxidizer Diversity in the Environment Using High-Throughput Sequencing

Nitrate leaching and its implication for Fe and As mobility in a Southeast Asian aquifer

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