Effects of Artemisinin Derivative on the Growth Metabolism of Tetrahymena thermophila BF5 Based on Expression of Thermokinetics

Xuesong, Shen; Qi, Shihua; Zhuangping, Qiu; Xu, Jingyi; Xie, Yanjun; Liu, Han-Fu; Liu, Yi

doi:10.1007/s12011-009-8527-2

Cited by 4 publications

(2 citation statements)

References 15 publications

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“…The antiparasitic potential of ART has previously been described in ciliates such as Tetrahymena thermophila, in which growth was modulated (Shen et al. ). The effect of antimalarial drugs on P. dicentrarchi may occur at the levels of pH homeostasis, as CQ has been described as an alkalinizing agent in several parasites (Gazarini et al.…”

Section: Discussionmentioning

confidence: 97%

Enzymes Involved in Pyrophosphate and Calcium Metabolism as Targets for Anti‐scuticociliate Chemotherapy

Mallo

Lamas

Defelipe

et al. 2016

J Eukaryotic Microbiology

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Inorganic pyrophosphate (PPi) is a key metabolite in cellular bioenergetics under chronic stress conditions in prokaryotes, protists and plants. Inorganic pyrophosphatases (PPases) are essential enzymes controlling the cellular concentration of PPi and mediating intracellular pH and Ca(2+) homeostasis. We report the effects of the antimalarial drugs chloroquine (CQ) and artemisinin (ART) on the in vitro growth of Philasterides dicentrarchi, a scuticociliate parasite of turbot; we also evaluated the action of these drugs on soluble (sPPases) and vacuolar H+-PPases (H+-PPases). CQ and ART inhibited the in vitro growth of ciliates with IC50 values of respectively 74 ± 9 μM and 80 ± 8 μM. CQ inhibits the H+ translocation (with an IC50 of 13.4 ± 0.2 μM), while ART increased translocation of H+ and acidification. However, both drugs caused a decrease in gene expression of H+-PPases. CQ significantly inhibited the enzymatic activity of sPPases, decreasing the consumption of intracellular PPi. ART inhibited intracellular accumulation of Ca(2+) induced by ATP, indicating an effect on the Ca(2+) -ATPase. The results suggest that CQ and ART deregulate enzymes associated with PPi and Ca(2+) metabolism, altering the intracellular pH homeostasis vital for parasite survival and providing a target for the development of new drugs against scuticociliatosis.

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Section: Discussionmentioning

confidence: 97%

Enzymes Involved in Pyrophosphate and Calcium Metabolism as Targets for Anti‐scuticociliate Chemotherapy

Mallo

Lamas

Defelipe

et al. 2016

J Eukaryotic Microbiology

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“…The in vitro hepatoma HepG-2 cells in the log phase were harvested, single-cell suspensions were prepared at a concentration of 1 Â 10 7 viable cells/ml, and the mice were injected subcutaneously in the left flank with 0.2 ml of the cell suspension (Bar-On et al, 2007;Shen et al, 2010;Chernysh et al, 2012). The mice were observed everyday.…”

Section: Establishment Of a Hepatocellular Carcinoma (Hcc) Xenograft mentioning

confidence: 99%

In vivostudy of effects of artesunate nanoliposomes on human hepatocellular carcinoma xenografts in nude mice

et al. 2013

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To investigate the effect of artesunate nanoliposomes on cultured cells in vitro and hepatocellular carcinoma xenografts in BALB/c-nu mice. Fluorescence polarization was applied for measurement of mitochondrial membrane fluidities; inhibition test of tumor cell proliferation in vitro was performed and nude mice xenograft model from human hepatocellular carcinoma (HCC) was established. Cytotoxicity of these compounds was evaluated by MTT assay on hepatocellular carcinoma xenografts in nude mice. Anisotropy (r-value) of blank nanoliposomes didn't change, it had no statistically significance between the blank nanoliposomes group and the control group, it indicated that artesunate had no obvious effect on L-O2 human normal liver cells. IC 50 values of artesunate nanoliposomes and artesunate API (active pharmaceutical ingredient) against HepG-2 cells were 15.997 and 19.706 mg/ml; IC 50 values of the same drugs against L-O2 normal human liver cells were 100.23 and 105.54 mg/ml, respectively. Tumor growth inhibitory effect of artesunate nanoliposomes was 32.7%, and artesunate API was 20.5%, respectively. HepG-2 cells treated with artesunate nanoliposomes showed dose-dependent apoptosis. The antitumor effect of artesunate nanoliposomes on human hepatoma HepG2 cells were stronger than that of artesunate API at the same concentration.

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Transcriptome analysis of Tetrahymena thermophila response to exposure with dihydroartemisinin

Pan

Deng

Zhang

et al. 2023

Heliyon

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Effects of Artemisinin Derivative on the Growth Metabolism of Tetrahymena thermophila BF5 Based on Expression of Thermokinetics

Abstract: The toxic effects of artesunate and dihydroartemisinin on the growth metabolism of Tetrahymena thermophila BF5 were studied by microcalorimetry. The results showed that: (1) low concentrations of artesunate ( Show more

Cited by 4 publications

References 15 publications

Enzymes Involved in Pyrophosphate and Calcium Metabolism as Targets for Anti‐scuticociliate Chemotherapy

Enzymes Involved in Pyrophosphate and Calcium Metabolism as Targets for Anti‐scuticociliate Chemotherapy

In vivostudy of effects of artesunate nanoliposomes on human hepatocellular carcinoma xenografts in nude mice

Transcriptome analysis of Tetrahymena thermophila response to exposure with dihydroartemisinin

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