Effects of artificial micro- and nano-structured surfaces on cell behaviour

Martínez, Elena; Engel, Elisabeth; Planell, Josep A.; Samitier, J.

doi:10.1016/j.aanat.2008.05.006

Cited by 365 publications

(289 citation statements)

References 53 publications

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“…during embryogenesis, tissue formation or regeneration) [1][2][3] and can be exploited to manipulate cell morpho-functional responses in vitro [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Interaction of leech neurons with topographical gratings: comparison with rodent and human neuronal lines and primary cells

et al. 2014

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Controlling and improving neuronal cell migration and neurite outgrowth are critical elements of tissue engineering applications and development of artificial neuronal interfaces. To this end, a promising approach exploits nano/microstructured surfaces, which have been demonstrated to be capable of tuning neuronal differentiation, polarity, migration and neurite orientation. Here, we investigate the neurite contact guidance of leech neurons on plastic gratings (GRs; anisotropic topographies composed of alternating lines of grooves and ridges). By high-resolution microscopy, we quantitatively evaluate the changes in tubulin cytoskeleton organization and cell morphology and in the neurite and growth cone development. The topography-reading process of leech neurons on GRs is mediated by filopodia and is more responsive to 4-µm-period GRs than to smaller period GRs. Leech neuron behaviour on GRs is finally compared and validated with several other neuronal cells, from murine differentiated embryonic stem cells and primary hippocampal neurons to differentiated human neuroblastoma cells.

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“…during embryogenesis, tissue formation or regeneration) [1][2][3] and can be exploited to manipulate cell morpho-functional responses in vitro [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Interaction of leech neurons with topographical gratings: comparison with rodent and human neuronal lines and primary cells

et al. 2014

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“…Substrate topography comprised of various microand nanofeatures influences cellular behavior [46]. Dalby et al [19] showed that random circular nanostructures with a diameter of approximately 120 nm promoted and directed osteogenic differentiation of HMSCs in the absence of osteogenic supplements in cell culture media, suggesting that nanofeatures alone can stimulate osteogenic differentiation and mineral production in vitro.…”

Section: Discussionmentioning

confidence: 99%

Nano-ceramic Composite Scaffolds for Bioreactor-based Bone Engineering

Deng

Ulery

et al. 2013

Clinical Orthopaedics &Amp; Related Research

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Background Composites of biodegradable polymers and bioactive ceramics are candidates for tissue-engineered scaffolds that closely match the properties of bone. We previously developed a porous, three-dimensional poly (D,L-lactide-co-glycolide) (PLAGA)/nanohydroxyapatite (n-HA) scaffold as a potential bone tissue engineering matrix suitable for high-aspect ratio vessel (HARV) bioreactor applications. However, the physical and cellular properties of this scaffold are unknown. The present study aims to evaluate the effect of n-HA in modulating PLAGA scaffold properties and human mesenchymal stem cell (HMSC) responses in a HARV bioreactor. Questions/purposes By comparing PLAGA/n-HA and PLAGA scaffolds, we asked whether incorporation of n-HA (1) accelerates scaffold degradation and compromises mechanical integrity; (2) promotes HMSC proliferation and differentiation; and (3) enhances HMSC mineralization when cultured in HARV bioreactors. Methods PLAGA/n-HA scaffolds (total number = 48) were loaded into HARV bioreactors for 6 weeks and monitored for mass, molecular weight, mechanical, and morphological changes. HMSCs were seeded on PLAGA/ n-HA scaffolds (total number = 38) and cultured in HARV bioreactors for 28 days. Cell migration, proliferation, osteogenic differentiation, and mineralization were characterized at four selected time points. The same amount of PLAGA scaffolds were used as controls.

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“…The fiber diameter of such 3D scaffold was measured to be a mixture of micro-(3.3 ± 0.6 µm) and nano-(240 ± 50 nm) fibers. The collected scaffold also replicates the inherent micro-nanoscale features in ECM that is essential in triggering series of cell activities [31][32][33][34][35][36] . The scaffold is therefore termed 3D multi-scale scaffold herein.…”

Section: Fabrication Of 3d Multi-scale Scaffold Using Hypodermic Needmentioning

confidence: 99%

Electrospun 3D multi-scale fibrous scaffold for enhanced human dermal fibroblasts infiltration

Leong

et al. 2024

IJB

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Electrospun polymeric nanofibrous scaffold possesses significant potential in the field of tissue engineering due to its extracellular matrix mimicking topographical features that modulate a variety of key cellular activities. However, traditional two-dimensional (2D) electrospun scaffolds are generally close-packed fiber mats which prohibit cell infiltration and proliferation. Consequently, the applications of electrospun scaffolds in regenerative medicine are limited. In this study, we detail the use of a needle collector to fabricate three-dimensional (3D) electrospun poly-ε-caprolactone (PCL) scaffolds with multi-scale fiber dimensions. The resultant pore size is 4 times larger than conventional 2D electrospun scaffolds with interweaving micro (3.3 ± 0.6 µm) and nano (240 ± 50 nm) fibers. The scaffold was surface modified by grafting with gelatin molecules. It was found that surface modification significantly improved human dermal fibroblasts (HDFs) cell infiltration throughout the 3D multi-scale scaffold. Even after an extended culture period of up to 28 days, cell proliferation was well supported in the surface-modified 3D multi-scale scaffold as confirmed by Ki67 staining. Extracellular matrix proteins secreted by the HDFs was evident on the 3D multi-scale PCL scaffold showing promising potential to facilitate tissue regeneration, in particular dermal tissue engineering.

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Effects of artificial micro- and nano-structured surfaces on cell behaviour

Cited by 365 publications

References 53 publications

Interaction of leech neurons with topographical gratings: comparison with rodent and human neuronal lines and primary cells

Interaction of leech neurons with topographical gratings: comparison with rodent and human neuronal lines and primary cells

Nano-ceramic Composite Scaffolds for Bioreactor-based Bone Engineering

Electrospun 3D multi-scale fibrous scaffold for enhanced human dermal fibroblasts infiltration

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