Effects of astaxanthin and canthaxanthin on oxidative stress biomarkers in rainbow trout

Elia, Antonia Concetta; Prearo, Marino; Dörr, Ambrosius Josef Martin; Pacini, Nicole; Magara, Gabriele; Brizio, Paola; Gasco, Laura; Abete, Maria Cesarina

doi:10.1080/15287394.2019.1648346

Cited by 32 publications

(26 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Concerning the effect of dietary AX supplementation on endogenous antioxidant enzymes (both in terms of activities and mRNA level of GR), the enzyme involved in the reduction of GSSG to GSH using NADPH as a reducing cofactor [60] was increased, whereas other antioxidant enzymes were not significantly affected. Similar to our results, an enhanced GR activity was reported in liver of rainbow trout after 8 weeks of AX supplementation [12].…”

Section: Control Of Antioxidant Enzymes By Episodic Hyperoxia and Diesupporting

confidence: 92%

“…The significant increase of GSH/GSSG could be related to the fact that glutathione is recycled in a NADPH-dependent reaction by GR, and the GR activity was significantly increased in fish fed the ASTA diet. Similarly, in a study with rainbow trout fed an AX-supplemented diet for 8 weeks, elevated liver GR activity allowed liver GSH amounts to be sustained [12]. In addition, NADPH synthesis seems to be augmented by the ASTA diet through the increase in g6pd mRNA level.…”

Section: Control Of Glutathione Metabolism By Episodic Hyperoxia and mentioning

confidence: 92%

“…Moreover, it can effectively scavenge lipid radicals and destroy peroxide chain reactions to protect polyunsaturated fatty acids (PUFAs) and sensitive membranes [9].Besides its role in pigmentation, a few studies have focused on the role of AX on fish health [10], and to our knowledge not much work has been undertaken to assess the potential antioxidant function of this xanthophyll. In rainbow trout, dietary AX enhanced plasma [11], liver, and kidney antioxidant defense [12]. Similarly, in European seabass fry, dietary AX together with sodium taurocholate reduced lipid peroxidation and increased total antioxidant status [13].…”

mentioning

confidence: 93%

See 2 more Smart Citations

Influence of Dietary Astaxanthin on the Hepatic Oxidative Stress Response Caused by Episodic Hyperoxia in Rainbow Trout

et al. 2019

View full text Add to dashboard Cite

A 13-week feeding trial was carried out with juvenile rainbow trout to test two diets: a control diet without astaxanthin (AX) supplementation (CTRL diet), and a diet supplemented with 100 mg/kg of synthetic AX (ASTA diet). During the last week of the feeding trial, fish were exposed to episodic hyperoxia challenge for 8 consecutive hours per day. Episodic hyperoxia induced physiological stress responses characterized by a significant increase in plasma cortisol and hepatic glycogen and a decrease in plasma glucose levels. The decrease of plasma glucose and the increase of hepatic glycogen content due to episodic hyperoxia were emphasized with the ASTA diet. Hyperoxia led to an increase in thiobarbituric acid-reactive substances in the muscle, diminished by dietary AX supplementation in both liver and muscle. Muscle and liver AX were increased and decreased respectively after 7-day episodic hyperoxia, leading to an increase in flesh redness. This augment of muscle AX could not be attributed to AX mobilization, since plasma AX was not affected by hyperoxia. Moreover, hyperoxia decreased most of antioxidant enzyme activities in liver, whereas dietary AX supplementation specifically increased glutathione reductase activity. A higher mRNA level of hepatic glutathione reductase, thioredoxin reductase, and glutamate-cysteine ligase in trout fed the ASTA diet suggests the role of AX in glutathione and thioredoxin recycling and in de novo glutathione synthesis. Indeed, dietary AX supplementation improved the ratio between reduced and oxidized glutathione (GSH/GSSG) in liver. In addition, the ASTA diet up-regulated glucokinase and glucose-6-phosphate dehydrogenase mRNA level in the liver, signaling that dietary AX supplementation may also stimulate the oxidative phase of the pentose phosphate pathway that produces NADPH, which provides reducing power that counteracts oxidative stress. The present results provide a broader understanding of the mechanisms by which dietary AX is involved in the reduction of oxidative status. very much linked to oxidative stress, which is the result of an imbalance between the production of reactive oxygen (ROS) and nitrogen species (RNS) by cell respiration and immune responses, and the state of the antioxidant defenses [1]. In order to protect against oxidative stress, organisms have developed antioxidant systems consisting of low-molecular-mass compounds including glutathione, ascorbic and uric acid, tocopherols, and carotenoids, and high-molecular-mass proteins including superoxide dismutases, catalases, Se-dependent glutathione peroxidases, glutathione reductase, and glucose-6-phosphate dehydrogenase [2].Carotenoids are natural pigments with immune-stimulant and antioxidant properties [3,4] and are present in the integument of many vertebrate species, generating bright-colored traits [5] Among carotenoids, astaxanthin (AX) is the most commonly used feed additive in order to achieve the characteristic red-pink coloration in crustaceans and salmonids [6,7]. The red coloration of AX is due ...

show abstract

Section: Control Of Antioxidant Enzymes By Episodic Hyperoxia and Diesupporting

confidence: 92%

Section: Control Of Glutathione Metabolism By Episodic Hyperoxia and mentioning

confidence: 92%

mentioning

confidence: 93%

See 1 more Smart Citation

Influence of Dietary Astaxanthin on the Hepatic Oxidative Stress Response Caused by Episodic Hyperoxia in Rainbow Trout

et al. 2019

View full text Add to dashboard Cite

show abstract

“…In aquatic species, the levels of those biomolecules involved in antioxidant and detoxifying defence can be used as informative biomarkers to investigate the oxidative stress exerted by xenobiotics (Al Kaddissi et al 2012Kaddissi et al , 2014Cozzari et al 2015;Elia et al 2007Elia et al , 2017Elia et al , 2018Elia et al , 2019Elia et al , 2020Magara et al 2018Magara et al , 2019. Metallothioneins (MTs) are proteins able to bind heavy metals, acting as homeostatic buffers.…”

Section: Introductionmentioning

confidence: 99%

Metal load and oxidative stress driven by organotin compounds on rainbow trout

Magara

Elia

Dörr

et al. 2021

Environ Sci Pollut Res

Self Cite

View full text Add to dashboard Cite

Tributyltin-based (TBT) antifouling paints, widely used for the treatment of flooded surfaces, have been banned in 2008 for their high environmental persistence and bioaccumulation in aquatic organisms. Although it is still present in aquatic ecosystems, oxidative stress driven by TBT has been still poorly investigated in fish. The aim of the study was to examine the time-course stress responses in liver of rainbow trout that received a single intraperitoneal injection of tributyltin chloride (TBTC) or tributyltin ethoxide (TBTE), both at a dose of 0.05 and 0.5 mg/kg. Levels of metallothioneins, total glutathione, malondialdehyde, superoxide dismutase, catalase, glutathione peroxidase and glutathione S-transferase were evaluated at 3 and 6 days post-injection. Tin load was measured in the muscle of the same fish. Differences were observed in the time-course accumulation of tin with a clear dose-response relationship. Although individual oxidative stress biomarkers varied, the biomarker profile indicated different stress mechanisms caused by both TBTC and TBTE. The weak induction of metal-trapping metallothioneins and the changes of oxidative stress biomarkers suggested a stress-pressure in both TBT-treated trout, advising for an ecotoxicological risk for freshwater ecosystems.

show abstract

“…Studies have shown that canthaxanthin is capable of scavenging reactive oxygen species and quenching singlet oxygen [ 9 , 27 ]. In addition, in vivo experiments revealed that canthaxanthin supplementation led to a decrease in lipid peroxidation to prevent induced liver DNA damage in rats [ 28 ] and to enhance the antioxidant defense in rat liver [ 29 , 30 ]. At the immunomodulatory level, an interesting example was observed in a mouse cell line, in which canthaxanthin increased the activity of alkaline phosphatase, an enzyme found in several tissues involved in the detoxification of lipopolysaccharides.…”

Section: Introduction: Characterization and Biosynthesis Of Canthamentioning

confidence: 99%

Canthaxanthin, a Red-Hot Carotenoid: Applications, Synthesis, and Biosynthetic Evolution

Rebelo

Farrona

Ventura

et al. 2020

Plants

View full text Add to dashboard Cite

Carotenoids are a class of pigments with a biological role in light capture and antioxidant activities. High value ketocarotenoids, such as astaxanthin and canthaxanthin, are highly appealing for applications in human nutraceutical, cosmetic, and animal feed industries due to their color- and health-related properties. In this review, recent advances in metabolic engineering and synthetic biology towards the production of ketocarotenoids, in particular the red-orange canthaxanthin, are highlighted. Also reviewed and discussed are the properties of canthaxanthin, its natural producers, and various strategies for its chemical synthesis. We review the de novo synthesis of canthaxanthin and the functional β-carotene ketolase enzyme across organisms, supported by a protein-sequence-based phylogenetic analysis. Various possible modifications of the carotenoid biosynthesis pathway and the present sustainable cost-effective alternative platforms for ketocarotenoids biosynthesis are also discussed.

show abstract

Effects of astaxanthin and canthaxanthin on oxidative stress biomarkers in rainbow trout

Cited by 32 publications

References 47 publications

Influence of Dietary Astaxanthin on the Hepatic Oxidative Stress Response Caused by Episodic Hyperoxia in Rainbow Trout

Influence of Dietary Astaxanthin on the Hepatic Oxidative Stress Response Caused by Episodic Hyperoxia in Rainbow Trout

Metal load and oxidative stress driven by organotin compounds on rainbow trout

Canthaxanthin, a Red-Hot Carotenoid: Applications, Synthesis, and Biosynthetic Evolution

Contact Info

Product

Resources

About