Effects of Atrazine on the Development of Neural System of Zebrafish,<i>Danio rerio</i>

Wang, Hao; Mu, Shumei; Zhang, Fengjuan; Wang, Hailin; Liu, Huan; Zhang, Han; Kang, Xianjiang

doi:10.1155/2015/976068

Cited by 11 publications

(6 citation statements)

References 44 publications

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Section: Discussionmentioning

confidence: 99%

“…All four studies had null findings. Although there are no epidemiological studies of atrazine and eye defects, animal studies have reported abnormal eye development with exposure to atrazine (Scahill 2008;Wang et al 2015). PAX6 is important for glucose metabolism, and disruption of PAX6 expression increases the risk of diabetes (Panneerselvam et al 2019).…”

Section: Discussionmentioning

confidence: 99%

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Epigenome-Wide DNA Methylation and Pesticide Use in the Agricultural Lung Health Study

Hoang

Paul

et al. 2021

Environ Health Perspect

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Background: Pesticide exposure is associated with many long-term health outcomes; the potential underlying mechanisms are not well established for most associations. Epigenetic modifications, such as DNA methylation, may contribute. Individual pesticides may be associated with specific DNA methylation patterns but no epigenome-wide association study (EWAS) has evaluated methylation in relation to individual pesticides. Objectives: We conducted an EWAS of DNA methylation in relation to several pesticide active ingredients. Methods: The Agricultural Lung Health Study is a case–control study of asthma, nested within the Agricultural Health Study. We analyzed blood DNA methylation measured using Illumina’s EPIC array in 1,170 male farmers of European ancestry. For pesticides still on the market at blood collection (2009–2013), we evaluated nine active ingredients for which at least 30 participants reported past and current (within the last 12 months) use, as well as seven banned organochlorines with at least 30 participants reporting past use. We used robust linear regression to compare methylation at individual C-phosphate-G sites (CpGs) among users of a specific pesticide to never users. Results: Using family-wise error rate ( ) or false-discovery rate ( ), we identified 162 differentially methylated CpGs across 8 of 9 currently marketed active ingredients (acetochlor, atrazine, dicamba, glyphosate, malathion, metolachlor, mesotrione, and picloram) and one banned organochlorine (heptachlor). Differentially methylated CpGs were unique to each active ingredient, and a dose–response relationship with lifetime days of use was observed for most. Significant CpGs were enriched for transcription motifs and 28% of CpGs were associated with whole blood cis -gene expression, supporting functional effects of findings. We corroborated a previously reported association between dichlorodiphenyltrichloroethane (banned in the United States in 1972) and epigenetic age acceleration. Discussion: We identified differential methylation for several active ingredients in male farmers of European ancestry. These may serve as biomarkers of chronic exposure and could inform mechanisms of long-term health outcomes from pesticide exposure. https://doi.org/10.1289/EHP8928

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Epigenome-Wide DNA Methylation and Pesticide Use in the Agricultural Lung Health Study

Hoang

Paul

et al. 2021

Environ Health Perspect

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“…Atrazine is a commonly used herbicide that prevents the growth of broad leaf and grassy weeds and also impacts vertebrate development (Song et al, 2014; Wang et al, 2015; Wirbisky et al, 2016a). Although it has been banned by the European Union (Sass and Colangelo, 2006), atrazine is still widely used across the United States and countries in Asia on plants such as corn, sugarcane, cotton, and pineapple.…”

Section: Introductionmentioning

confidence: 99%

Atrazine affects craniofacial chondrogenesis and axial skeleton mineralization in zebrafish (Danio rerio)

2018

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Atrazine is a commonly used herbicide that has previously been implicated as an endocrine-disrupting compound. Previous studies have shown that estrogenic endocrine-disrupting compounds affect the development of the heart, cartilage, and bone in zebrafish ( Danio rerio). To determine whether atrazine has effects similar to other endocrine disruptors, zebrafish embryos were treated with a range of atrazine concentrations. Atrazine treatment at a low concentration of 0.1 µM resulted in significant differences in craniofacial cartilage elements, while concentrations ≥1 µM led to decreased survival and increased heart rates. Fish treated with ≥1 µM atrazine also developed with delayed vertebrae mineralization. Higher concentrations of atrazine caused gross craniofacial defects and decreased hatching rates. Further studies into the molecular pathways disrupted in these developmental processes could shed light on a link between endocrine-disrupting compounds and developmental abnormalities.

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“…Chronic ATR exposure results in high accumulative levels in multiple organs and tissues [10-14]. It has been shown that ATR and its metabolites, known as environmental endocrine disruptors, affect the central nervous and reproductive systems [15-17]. In terms of its nervous system toxicity, ATR decreases the level of striatal dopamine (DA) and the number of tyrosine hydroxylase (TH)-positive dopaminergic neurons in rodents, resulting in pathology and behavior similar to that of a Parkinson’s disease (PD) model.…”

Section: Introductionmentioning

confidence: 99%

Isoflavones Induce BEX2-Dependent Autophagy to Prevent ATR-Induced Neurotoxicity in SH-SY5Y Cells

Ma¹,

Wu²,

Wu³

et al. 2017

Cell Physiol Biochem

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Background/Aims: Atrazine (ATR) is a broad-spectrum herbicide in wide use around the world. However, ATR is neurotoxic and can cause cell death in dopaminergic neurons, leading to neurodegenerative disorders. Autophagy is the basic cellular catabolic process involving the degradation of proteins and damaged organelles. Studies have shown that certain plant compounds can induce autophagy and prevent neuronal cell death. This prompted us to investigate plant compounds that might reduce the neurotoxic effects of ATR. Methods: By CCK-8 and flow cytometry, we tested the ability of five candidate compounds—isoflavones, resveratrol, quercetin, curcumin, and green tea polyphenols—to protect cells from ATR. Changes in the expression of tyrosine hydroxylase (TH) and brain-expressed X-linked 2 (BEX2), autophagy-related proteins and key factors in mTOR signaling, were detected by Western blotting. Results: Isoflavones had the strongest activity against ATR-induced neuronal apoptosis. ATR reduced the expression of TH and BEX2, whereas isoflavones increased TH and BEX2 expression. In addition, ATR inhibited autophagy, whereas isoflavones induced autophagy through the accumulation of LC3-II and decreased expression of p62; this effect was abolished by 3-methyladenine (3-MA). Furthermore, BEX2 siRNA abolished isoflavone-mediated autophagy and neuroprotection in vitro. Conclusion: Isoflavones activate BEX2-dependent autophagy, protecting against ATR-induced neuronal apoptosis.

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Effects of Atrazine on the Development of Neural System of Zebrafish,Danio rerio

Cited by 11 publications

References 44 publications

Epigenome-Wide DNA Methylation and Pesticide Use in the Agricultural Lung Health Study

Epigenome-Wide DNA Methylation and Pesticide Use in the Agricultural Lung Health Study

Atrazine affects craniofacial chondrogenesis and axial skeleton mineralization in zebrafish (Danio rerio)

Isoflavones Induce BEX2-Dependent Autophagy to Prevent ATR-Induced Neurotoxicity in SH-SY5Y Cells

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