Effects of autologous platelet concentrate and serum on retinal wound healing in an animal model

Cullinane, Anthony; O'Callaghan, Pat; McDermott, Kieran W.; Keohane, Catherine; Cleary, Philip E.

doi:10.1007/s00417-001-0397-z

Cited by 9 publications

(10 citation statements)

References 28 publications

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“… 5 , 16 , 17 , 38 As in some cases described by other authors, 14 – 17 two of our patients presented with a break in the tissue overlying the ODP, and the vitreous remained attached in both cases, visualized before and during surgery. We believe that the use of APC with its mitogenic and chemotactic properties, 39 associated with posterior hyaloid peeling to eliminate vitreomacular traction, favors the proliferative cellular response and closure of the suspected communication between the vitreous and the retina and, secondarily, promotes reabsorption of intra- and subretinal fluid.…”

Section: Discussionmentioning

confidence: 99%

Autologous platelet concentrate in surgery for macular detachment associated with congenital optic disc pit

Nadal¹,

Figueroa²,

Carreras³

et al. 2015

OPTH

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PurposeTo evaluate the anatomical and functional results obtained with pars plana vitrectomy (PPV) plus autologous platelet concentrate (APC) as a treatment for macular detachment associated with optic disc pit (ODP).MethodsWe performed a prospective interventional study of 19 eyes of 19 consecutive patients with posterior macular detachment due to ODP. All patients underwent PPV, posterior hyaloid peeling, fluid–air exchange, injection of 0.05 mL of APC over the ODP and 15% perfluoropropane (C3F8) endotamponade. Postoperative measures included face-up positioning for 2 hours and then avoidance of the face-up position during the ensuing 10 days. All patients underwent complete ophthalmologic examination and optical coherence tomography preoperatively at 1 month, 3 months, 6 months, 9 months, and 12 months postoperatively and then annually. Outcome measures were best corrected visual acuity (BCVA) by logMAR, improvement of quality of vision, macular attachment, and resolution of intraretinal schisis-like separation.ResultsPreoperatively, the median BCVA was 0.70 (range: 0.30–1.70) and all patients showed improved visual acuity after surgery; BCVA was 0.22 (range: 0.07–0.52) at 12 months follow-up. All patients showed complete reabsorption of intraretinal fluid (median time: 3.5 months [range: 2–8 months]) and macular attachment at the end of follow-up (median: 60 months [range: 12–144 months]), with stable or improved visual acuity. No reoperations were needed and no major adverse events were recorded.ConclusionFor macular detachment associated with ODP, the combination of PPV, posterior hyaloid peeling, APC, and C3F8 tamponade is a highly effective alternative technique with stable anatomical and functional results.

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Section: Discussionmentioning

confidence: 99%

Autologous platelet concentrate in surgery for macular detachment associated with congenital optic disc pit

Nadal¹,

Figueroa²,

Carreras³

et al. 2015

OPTH

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“…For instance, autologous platelet releasate 14 as well as recombinant platelet-derived growth factors 15 may enhance healing of chronic lower extremity diabetic ulcers. Moreover, regeneration of cutaneous wounds, 16 retina, 17 and peri-implant bone 18 by platelets has been reported. However, treatment of surgical lesions with plateletrich plasma has also generated controversial results in clinical trials.…”

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confidence: 99%

Platelets in tissue repair: control of apoptosis and interactions with regenerative cells

Gawaz

Vogel

2013

Blood

173

129

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Besides mediating primary hemostasis and thrombosis, platelets play a critical role in tissue repair and regeneration. They regulate fundamental mechanisms involved in the healing process including cellular migration, proliferation, and angiogenesis. Control of apoptosis/cell survival and interaction with progenitor cells, which are clinically relevant but poorly understood aspects of platelets in tissue repair, will be highlighted in this review. Gaining deeper insight into the less well-characterized molecular mechanisms is necessary to develop new therapeutic platelet-based options.

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“…The combination of adipose tissue and PRP was found by some researchers to enhance graft adherence (Cullinane et al. 2002 ). In a respective cohort study estimating the effectiveness of platelet foot ulcers, the researchers found that the relative risk for a wound to heal after treatment was 95% when compared to the standard of care (Margolis et al.…”

Section: Discussionmentioning

confidence: 99%

Nanosecond Pulse Electric Field Activated-Platelet Rich Plasma Enhances the Return of Blood Flow to Large and Ischemic Wounds in a Rabbit Model

Hargrave

2015

Physiol Rep

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Platelet-rich plasma is a therapeutic strategy used for accelerating wound healing of a wide range of tissues through the release of platelet growth factors. Here, we describe a nonchemical, safe method for preparing platelet-rich plasma using nanosecond-pulsed electric fields (nsPEFs) and investigated the effect of this platelet-rich plasma on reperfusion of blood in large skin flap or ischemic hind limb wounds in New Zealand White rabbits. Laser Doppler images of blood flow to the dorsal surface of skin flap wounds or to ischemic hind limb wounds were obtained from wounds treated with 0.9% saline or nanosecond-pulsed electric field prepared platelet-rich plasma (nsPRP). Reperfusion in the skin flap wounds was greater in the nsPRP-treated wounds than in the wounds treated with saline on postoperative days 3 (P < 0.001) and 21 (P < 0.03). Reperfusion in the ischemic hind-limb treated with nsPRP was greater than in the saline-treated limb on post-operative Day 3 (P < 0.001), post-operative week 1 (P < 0.025) and post-operative week 4 (P < 0.015). In the hind limb ischemic tissue, the number of endothelial cells, collagen, and cells containing vascular endothelial growth factor (VEGF) was greater in the nsPRP-treated tissue. These results demonstrate that nsPRP improves blood flow in large surgical skin wounds and in ischemic wounds.

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Effects of autologous platelet concentrate and serum on retinal wound healing in an animal model

Abstract: We have demonstrated a significantly greater proliferative cellular response in the healing of retinal wounds with the use of platelet concentrate than in control wounds. We consider that this model may be used in further studies on the effects of other agents on retinal wound healing.

Cited by 9 publications

References 28 publications

Autologous platelet concentrate in surgery for macular detachment associated with congenital optic disc pit

Autologous platelet concentrate in surgery for macular detachment associated with congenital optic disc pit

Platelets in tissue repair: control of apoptosis and interactions with regenerative cells

Nanosecond Pulse Electric Field Activated-Platelet Rich Plasma Enhances the Return of Blood Flow to Large and Ischemic Wounds in a Rabbit Model

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