Effects of autologous platelet‑rich plasma injections on facial skin rejuvenation

Abstract

Autologous serum platelet-rich plasma (PRP) has been used to rejuvenate wrinkled and aged skin for years; however, the molecular mechanism for the positive effects of PRP on the skin remains unclear. The present study aimed to clarify the potential molecular mechanisms for the role of PRP in wrinkled and aged skin rejuvenation, and provide evidence for future clinical applications. A total of 30 healthy females were recruited for PRP treatment and signed informed consent was obtained. A total of 3 autologous PRP injections were administered to each patient with 15-day intervals between injections. The effects of PRP injections were evaluated using the VISIA ® Complexion Analysis System and skin computed tomography. A human organotypic skin model was established and treated with PBS or PRP before ultraviolet (UV)-B light (10 mJ/cm 2) irradiation. The distribution of the epidermal structure and dermal fibers were analyzed by hematoxylin and eosin and Masson's trichome staining. Expression of matrix metalloproteinase-1 (MMP-1), tyrosinase, fibrillin and tropoelastin was detected by reverse transcription-quantitative PCR, western blotting and immunofluorescence. The present results showed that PRP treatment improved skin quality in the participants. In addition, the VISIA ® results showed that wrinkles, texture and pores were decreased in the PRP groups compared with the PBS treatment. The in vitro study demonstrated that PRP treatment ameliorated photoaging by inhibiting UV-B-induced MMP-1 and tyrosinase upregulation, and by inducing fibrillin and tropoelastin expression that was downregulated by UV-B. Collectively, it was demonstrated that PRP treatment ameliorated skin photoaging through regulation of MMP-1, tyrosinase, fibrillin and tropoelastin expression.

Injectable platelet‐rich fibrin for facial rejuvenation: A prospective, single‐center study

“…A significant improvement in the percentile scores was observed in skin surface spots and pores with a numerical improvement for most of the other parameters. Improvement in these parameters assessed using the VISIA ® system has also been observed in other studies evaluating the efficacy of PRP injections for facial skin rejuvenation 41,42 …”

“…Improvement in these parameters assessed using the VISIA ® system has also been observed in other studies evaluating the efficacy of PRP injections for facial skin rejuvenation. 41,42 In this study, the FACE-Q patient-reported outcome tool was completed by patients at baseline and at the end of treatment. In view of the low baseline score for skin adverse effects (29 of 100), the lack of a statistically significant improvement is not unexpected.…”

The Anti‐photoaging Effects of Pre‐ and Post‐treatment of Platelet‐rich Plasma on UVB‐damaged HaCaT Keratinocytes

“…Besides, PRP can stimulate collagen synthesis and epidermal thickening, thus reducing wrinkles and repairing other signs of facial aging (8) To this end, PRP has been used in the treatment of facial rejuvenation. Although PRP has been reported to be effective in repairing photoaging skin in clinical trials (9), increasing studies are still focusing on elucidating the molecular mechanism of PRP on repairing photoaging skin (10,11).…”

Evaluating the Safety of Intravenous Delivery of Autologous Activated Platelet-rich Plasma

“…Platelet-rich plasma (PRP) is a high platelet concentrate extracted from the processed autologous plasma of the whole blood (1). A total of more than 1100 proteins have been found in PRP with different functions, ranging from enzymes, growth factors, and messengers of the immune system (2). These proteins and many bioactive factors are mainly secreted by three types of granule (alpha, delta, lambda) within the platelets, with alpha granules being the most abundant.…”

“…E-mail: karina@hayandra.com Submitted: 04 March 2021/Accepted: 26 April 2021 DOI: https://doi.org/10.17532/jhsci.2021.1276 cellular proliferation and differentiation, matrix remodeling, and angiogenesis. These biological processes are found to enhance wound healing and tissue regeneration (2).…”