Effects of bacterial cellulose gel on the anorectal resting pressures in rats submitted to anal sphincter injury

Cavalcante, Aline Ribeiro Teixeira; Lima, Rodrigo Pontes de; Souza, Veridiana Sales Barbosa de; Pinto, Flávia Cristina Morone; Júnior, Olávio Campos; Silva, Jaiurte Gomes Martins da; Albuquerque, Amanda Vasconcelos de; Aguiar, José Lamartine de Andrade

doi:10.1016/j.heliyon.2018.e01058

Cited by 7 publications

(7 citation statements)

References 29 publications

(37 reference statements)

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“…BC hydrogel subcutaneous implants were found beneath the hypodermis layer of the skin, a slight inflammatory response was present and the formation of new vessels (neovascularization) was present in the majority of the animals. These effects are supported by previous studies (Rangel et al, 2006;Carvalho Junior et al, 2012;Cordeiro-Barbosa et al, 2012;Lima et al, 2015;Pita et al, 2015;Lima et al, 2017;Cavalcante et al, 2018;Gonçalves-Pimentel et al, 2018).…”

Section: Discussionsupporting

confidence: 87%

“…The bacterial cellulose remains at the injection site (anorectal region), promoted neovascularization and the implant area was colonized by multinucleated giant cells, fibroblasts and dense conjunctive tissue associate to collagen fibers (Cavalcante et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Experimental and clinical studies suggested that sugarcane bacterial cellulose hydrogel (BC) is a biomaterial useful for a variety of surgical procedures, such as correction of hypospadias and other uses in urologic surgery (Martins et al, 2013;Lima et al, 2015;Lucena et al, 2015;Lima et al, 2017), treatment of fecal incontinence after anal trauma (Cavalcante et al, 2018), treatment of eviscerated eyes (Cordeiro-Barbosa et al, 2012), repair of tympanic membrane perforation (Silveira et al, 2016), vascular surgery procedures (Barros-Marques et al, 2012), pressure injury (Oliveira et al, 2019), orthopedic surgery (Albuquerque et al, 2011), and neurosurgery (Lima et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Non-clinical safety study of a sugarcane bacterial cellulose hydrogel

Silva

Pinto

Oliveira

et al. 2020

RSD

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A hydrogel of bacterial cellulose of 0.8%, biopolymer produced from sugarcane molasses and synthesized from a bacteria called Zoogloea sp., was analyzed for its composition and tested by two routes of administration, subcutaneous and intraperitoneal, to clarify the local and systemic adverse effects after skin implantation in Wistar rats (24 males, 24 females, 55 days old). Analysis of two samples of BC films indicates carbon (42.94%; 43.43%), hydrogen (6.73%; 6.76%), nitrogen (0.28%, 0.23%) and oxygen (50.05%, 49.58%). No abnormal behavior, clinical signs of chronic toxicity or inflammation were observed. There was no change in liver injury biomarker levels (ALT, AST and ALP), as well as in renal histology, where it was evaluated to signs of tubular cell injury, glomerular or vascular damage, and renal morphometry, in which it was quantified the number of renal corpuscles, the number of cells per glomerulus and the capsular space area. Thus, the absence of signs and symptoms of toxicity suggests that subcutaneous or intraperitoneal injections of these polymers may be used in clinical situations.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Non-clinical safety study of a sugarcane bacterial cellulose hydrogel

Silva

Pinto

Oliveira

et al. 2020

RSD

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“…Humans rely on cellulose for building material, clothing and fuel [1][2][3] . More recently the polymer has sparked interest in the biotechnology field as a potential source of biofuel feedstock 4 , and in the biomedical industry as a biologically neutral scaffold to promote tissue regeneration 5,6 .…”

Section: Introductionmentioning

confidence: 99%

Structure of the Bacterial Cellulose Ribbon and Its Assembly-Guiding Cytoskeleton by Electron Cryotomography

et al. 2021

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Cellulose is a widespread component of bacterial biofilms, where its properties of exceptional water retention, high tensile strength and stiffness prevents dehydration and mechanical disruption of the biofilm. Bacteria in the Gluconacetobacter genus secrete crystalline cellulose, with a structure very similar to that found in plant cell walls. How this higher-order structure is produced is poorly understood. We used cryo-electron tomography and focused ion beam milling of native bacterial biofilms to image cellulose-synthesizing G. hansenii and G. xylinus bacteria in a frozen-hydrated, near-native state. We confirm previous results suggesting that cellulose crystallization occurs serially following its secretion along one side of the cell, leading to a cellulose ribbon that can reach several microns in length and combine with ribbons from other cells to form a robust biofilm matrix. We were able to take direct measurements in a near-native state of the cellulose sheets. Our results also reveal a novel cytoskeletal structure, that we name the cortical belt, adjacent to the inner membrane and underlying the sites where cellulose is seen emerging from the cell. We find that this structure is not present in other cellulose-synthesizing bacterial species, Agrobacterium tumefaciens and Escherichia coli 1094, which do not produce organized cellulose ribbons. We therefore propose that the cortical belt holds the cellulose synthase complexes in a line, to form higher-order cellulose structures such as sheets and ribbons. Importance This work’s relevance for the microbiology community is two-fold: It delivers for the first time high-resolution near-native snapshots of the Gluconacetobacter spp. (previously Komagataibacter spp.) in the process of cellulose ribbon synthesis, in their native biofilm environment. It puts forward a non-characterized cytoskeleton element associated with the side of the cell where the cellulose synthesis occurs. This represents a step forward in the understanding of the cell-guided process of crystalline cellulose synthesis, particularly studied in the Gluconacetobacter genus and still not fully understood. Additionally, our successful attempt to cryo-FIB mill through biofilms to image the cells in their native environment will drive the community to use this tool for the morphological characterization of other studied biofilms.

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“…The use of bacterial cellulose (BC) gel using sugarcane molasses as substrate is based on numerous preclinical studies demonstrating that it is a stable polymer and is not absorbed by the surrounding tissues (7). It has low genotoxicity and cytotoxicity in vitro studies (8), high biocompatibility and integration in an in vivo model of gutted eyes (9); treatments for vesicoureteral reflux (10) and fecal incontinence (11). When comparing the BC gel implant to Deflux ® by endoscopy directly below the bladder neck, there was no inflammatory reaction and in the longterm better tissue integration (10).…”

Section: Introductionmentioning

confidence: 99%

Injectable semi rigid penile prosthesis: study in rabbits and future perspectives

Lima¹,

Chagas²,

Monteiro³

et al. 2021

Transl Androl Urol

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Background: Penile prostheses are the third option in the treatment of erectile dysfunction, however, despite their proven effectiveness, the occurrence of infections, advanced age of patients and comorbidities are the main limiting factors for this treatment modality. In the continuous search for biointegrated, clinically durable and minimally invasive treatment options, a possible model of penile prosthesis was sought through the use of intracavernous bacterial cellulose (BC) gel, in an experimental model of orchiectomized rabbits.Methods: Thirty adult New Zealand rabbits were equally distributed into three groups: BC; vehicle and control. Each group was then subdivided according to the follow-up time of 3 and 6 months.Bilateral orchiectomy was performed 3 weeks before injection in the BC and vehicle groups. Pachymetry measurements of the penile axis, diameter and length were performed in situ. Histomorphometry analyzes of the corpora cavernosa (CC), thickness of the tunica albuginea, cell density, collagen and elastic fibers postinjection were also performed, in addition to immunohistochemistry for newly formed vessels. Results:The implant of BC increased both the length and thickness of the penis three and six months after the last injection, with a consequent increase in the diameter of the CC. On the other hand, the filling effect was not observed in the control and vehicle groups, confirming the degradation of this tissue after orchiectomy and the effectiveness of BC as a filling agent. Histomorphometry analyzes corroborate the mass effect of BC integrated into the tissue, permeated by predominantly lymphomononuclear inflammatory infiltrate, multinucleated giant foreign body cells, fibroblasts, elastic fibers and newly formed vessels, without degradation or loss of volume, even after six months of implantation.Conclusions: Biocompatibility and biointegration to the host tissue make BC a prosperous penile filling material, with local application and minimally invasive.

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Effects of bacterial cellulose gel on the anorectal resting pressures in rats submitted to anal sphincter injury

Cited by 7 publications

References 29 publications

Non-clinical safety study of a sugarcane bacterial cellulose hydrogel

Non-clinical safety study of a sugarcane bacterial cellulose hydrogel

Structure of the Bacterial Cellulose Ribbon and Its Assembly-Guiding Cytoskeleton by Electron Cryotomography

Injectable semi rigid penile prosthesis: study in rabbits and future perspectives

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