Effects of Bacterial Coexistence on the Growth of a Marine Diatom &lt;i&gt;Chaetoceros gracilis&lt;/i&gt;

Suminto,; Hirayama, Kazutsugu

doi:10.2331/fishsci.62.40

Cited by 28 publications

(17 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Suminto & Hirayama (1993) studied the relationship between diatom growth and bacterial populations in small mass cultures, and reported an antagonistic relationship. Suminto & Hirayama (1996) recently isolated 12 strains of coexisting bacteria from prawn mass culture tanks, and investigated the effects of these bacteria on the growth of Chaetoceros gracilis. Among the bacteria tested, seven strains were suppressive, four had no effect, and only one strain, belonging to Flavobacterium sp., had stimulative effect on C. gracilis.…”

Section: Effects Of Concomitant Bacteria In Mass Culture Of Food Micrmentioning

confidence: 99%

Stimulative and inhibitory effects of bacteria on the growth of microalgae

Fukami

Nishijima

Ishida

1997

Live Food in Aquaculture

View full text Add to dashboard Cite

Several examples of stimulative and inhibitory effects of bacteria on microalgal growth are introduced, and the importance of bacteria in algal mass culture is investigated. Diatoms are often used as live food for planktonic larvae of sea urchin and bivalves. Monodispersed Chaetoceros ceratosporum has been cultivated by using clean, high nutrient content, deep seawater (DSW). However, the growth rate and cell yield of diatoms fluctuated, to relatively large extent, with the season that DSW was collected. When some bacterial strains isolated from DSW were added to the culture, diatom growth was often stimulated and a relatively constant cell yield was obtained. Another diatom species, C. gracilis, was also stimulated by adding some bacterial strains to cultures. The positive effect of bacteria on diatoms was observed not only for planktonic species, but also on attached species. A benthic diatom, Nitzschia sp., was stimulated by a bacterial film of Alcaligenes on the surface of the substratum. On the other hand, a strain of Flavobacterium sp. isolated from natural seawater during the decline period of an algal bloom had a strong algicidal effect on the red tide plankton, Gymnodinium mikimotoi. Recent reports demonstrate that many bacterial strains have significant algicidal effects on many species of red tide plankton. These results indicate that bacterial effects should be taken into account to obtain stable mass culture of food microalgae.

show abstract

Section: Effects Of Concomitant Bacteria In Mass Culture Of Food Micrmentioning

confidence: 99%

Stimulative and inhibitory effects of bacteria on the growth of microalgae

Fukami

Nishijima

Ishida

1997

Live Food in Aquaculture

View full text Add to dashboard Cite

show abstract

“…Furthermore, the marine bacterium Flavobacterium sp. increased the growth of the diatom obvious effects on the growth of Isochrysis galbana and Pavlova lutheri [24]. All of these results implied that the interactions between bacteria and microalgae are species-specific [12,18].…”

Section: Discussionmentioning

confidence: 99%

“…An overnight culture of 3-5 ml was centrifuged at 13,000 ×g for 1 min, after which the pellet was suspended in 500 μl of ex- Genet Bio) using 27F (5'-AGAGTT TGATCCTGGCTCAG-3') and 1492R (5'-GGTTACCTTGTTA CGACTT-3') [24] primers under the same PCR conditions as described above. The PCR product was sequenced using 16S rRNA gene primers (27F and 1492R).…”

Section: Identification Of Bacterial Isolatesmentioning

confidence: 99%

Growth Promotion of Pavlova viridis by Bacteria Isolated from the Microalga

Ahamed¹,

Kim²,

Tae-O³

et al. 2015

Journal of Life Science

View full text Add to dashboard Cite

The marine microalga Pavlova viridis can grow fast and has the ability to accumulate essential nutrients for culturing marine animals, such as EPA and DHA, and it has been used as food for raring larval fish and prawn. The symbiotic relationship between the flagellate microalga Pavlova viridis and its associated bacteria was investigated. An axenic culture of P. viridis was obtained by repeated treatment of the microalga with an antibiotic cocktail. The axenic status was confirmed after sub-culturing three times in a sterile f/2 medium without an antibiotic. The axenic alga was then co-inoculated with five bacteria, arbitrarily designated as I1-I5, isolated from the alga to test the growth promotion of the algae. All bacterial strains promoted the growth of P. viridis, and bacterial isolate I3 was the most effective among the five bacteria tested. The cell number of P. viridis in the co-culture with I3 was significantly higher than that of the control culture. A sequence analysis of the 16S rRNA gene isolated from I3 revealed a 97% nucleotide sequence similarity to that of Citrobacter sp. The growth of strain I3 was also significantly enhanced by co-culturing with P. viridis, indicating a symbiotic relationship between the microalga and its associated bacterium. The association between the microalga and bacterium was confirmed by scanning electron microscopy.

show abstract

“…This relation between a microscopic algae and bacteria is worth being handled experimentally and monitored because the optimal growth conditions of a microscopic alga usually requires specific accompanying bacteria (Suminto & Hirayama, 1996;Fukami et al, 1997). This close bacterial-algal association is very important.…”

Section: Discussionmentioning

confidence: 99%

Simultaneous enumeration of Phaeodactylum tricornutum (MLB292) and bacteria growing in mixed communities

et al. 2005

View full text Add to dashboard Cite

ABSTRACT. Diatoms play a decisive role in global primary production, have a key role in the marine food web, and serve as food for the industrial culture of aquatic animals. In addition, species of diatoms have been chosen as tools for measuring sea water quality and for bioassay procedures. Among them, Phaeodactylum tricornutum (Bohlin) Lewin, 1958, represents a good biological model due to its ease of handling and also to its physiological, genetic and growth characteristics. We investigated the use of the Epifluorescence Microscopy (EPM) to visualize and enumerate P. tricornutum cells and bacteria in cultures containing both kinds of microorganisms. The cell suspension was filtered, stained with SYBR Green and analyzed by EPM. P. tricornutum can be enumerated using EPM with comparable accuracy as using a hemocytometer. The same kind of growth curve is obtained with either method. EPM is an advantageous alternative to the classic light microscope counting method; it allows the simultaneous enumeration of P. tricornutum and bacteria in a single sample, measuring red autofluorescence and green fluorescence respectively. Moreover, the methodology is suitable for dynamic studies performed to determine the growth dependence between P. tricornutum and bacteria. Another advantage of this technique is that different shapes, sizes and physiological stages of the microbiological communities can be observed.Key words: microscopic algae, bacteria, growth, enumeration, epifluorescence.Enumeración simultánea del crecimiento de Phaeodactylum tricornutum (MLB292) y bacterias en comunidades mixtas RESUMEN. Las diatomeas tienen un rol decisivo en la producción primaria global, un rol clave en la red trófica marina y sirven como alimento en el cultivo industrial de animales acuáticos. Algunas especies han sido elegidas para medir la calidad del agua y para bioensayos. Entre ellas, Phaeodactylum tricornutum (Bohlin) Lewin, 1958, representa un buen modelo biológico debido a su fácil manejo, como también a sus características fisiológicas, genéticas y de crecimiento. Se analiza el uso de la Microscopía de Epifluorescencia (MEP) para visualizar y enumerar células de P. tricornutum y bacterias en cultivos que contienen ambos tipos de microorganismos. Las células en suspensión fueron filtradas, marcadas con SYBR Green y analizadas con MEP. Las células de P. tricornutum se pueden contar con una precisión comparable a la del hemocitómetro y se obtiene la misma curva de crecimiento con ambos métodos. La MEP es una alternativa ventajosa al uso del clásico microscopio de luz, ya que permite el recuento simultáneo de P. tricornutum y bacterias en una misma muestra, midiéndose la autofluorescencia roja y la fluorescencia verde, respectivamente. Más aún, la metodología es apropiada para estudios de dinámica y para determinar la dependencia entre el crecimiento de P. tricornutum y las bacterias. Otra ventaja de esta técnica es que se puede observar diferencias de forma, tamaño y estado fisiológicos de las comunidades microbiológicas.Palabras ...

show abstract

Effects of Bacterial Coexistence on the Growth of a Marine Diatom <i>Chaetoceros gracilis</i>

Cited by 28 publications

References 2 publications

Stimulative and inhibitory effects of bacteria on the growth of microalgae

Stimulative and inhibitory effects of bacteria on the growth of microalgae

Growth Promotion of Pavlova viridis by Bacteria Isolated from the Microalga

Simultaneous enumeration of Phaeodactylum tricornutum (MLB292) and bacteria growing in mixed communities

Contact Info

Product

Resources

About