Effects of Bambusae Caulis in Taeniam Extract on the UVB-induced Cell Death, Oxidative Stress and Matrix Metalloproteinase 1 Expression in Keratinocytes

Seok, Jin Kyung; Kwak, Jun Yup; Seo, Hyeong Ho; Suh, Hwa Jin; Boo, Yong Chool

doi:10.15230/scsk.2015.41.1.9

Cited by 8 publications

(17 citation statements)

References 38 publications

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“…HaCaT cells (a human immortalized keratinocyte cell line) were grown in Dulbecco's modified Eagle's medium/F-12 medium (GIBCO-BRL, Grand Island, NY, USA) supplemented with 10% fetal bovine serum, 100 U mL À1 penicillin, 0.1 mg mL À1 streptomycin, 0.25 μg mL À1 amphotericin B, and 10 μg mL À1 hydrocortisone. For experiments requiring UV exposure, HaCaT cells were seeded in a six-well plate at a density of 3 × 10 5 cells per well and grown for 24 h. Cells were then irradiated with UVB in phosphate buffered saline containing a test material at the specified concentrations, as previously described (Park et al, 2014;Seok et al, 2015). Irradiated cells were cultured with a growth medium for 24 h. Cell viability was determined using 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyltetrazolium bromide (Park et al, 2014;Seok et al, 2015).…”

Section: Methodsmentioning

confidence: 99%

“…For experiments requiring UV exposure, HaCaT cells were seeded in a six‐well plate at a density of 3 × 10 5 cells per well and grown for 24 h. Cells were then irradiated with UVB in phosphate buffered saline containing a test material at the specified concentrations, as previously described (Park et al ., ; Seok et al ., ). Irradiated cells were cultured with a growth medium for 24 h. Cell viability was determined using 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyltetrazolium bromide (Park et al ., ; Seok et al ., ).…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, there is a need for the development of safer and more effective natural ingredients for sun protection. Plant extracts containing UV-shielding and/or antioxidant properties are extremely attractive (Park et al, 2014;Seok et al, 2015;Svobodova et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Scutellaria radix Extract as a Natural UV Protectant for Human Skin

Seok

Kwak

Choi³

et al. 2015

Phytotherapy Research

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Ultraviolet (UV) radiation induces oxidative injury and inflammation in human skin. Scutellaria radix (SR, the root of Scutellaria baicalensis Georgi) contains flavonoids with high UV absorptivity and antioxidant properties. The purpose of this study was to examine the potential use of SR extract as an additive in cosmetic products for UV protection. SR extract and its butanol (BuOH) fraction strongly absorbed UV radiation and displayed free radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl radials and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radicals. They also attenuated the UV-induced death of HaCaT cells. Sunscreen creams, with or without supplementation of SR extract BuOH fraction, were tested in vivo in human trials to evaluate potential skin irritation and determine the sun protection factor (SPF). Both sunscreen creams induced no skin irritation. A sunscreen cream containing 24% ZnO showed an SPF value of 17.8, and it increased to 22.7 when supplemented with 5% SR extract BuOH fraction. This study suggests that SR-derived materials are useful as safe cosmetic additives that provide UV protection.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Scutellaria radix Extract as a Natural UV Protectant for Human Skin

Seok

Kwak

Choi³

et al. 2015

Phytotherapy Research

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“…Ferulic acid, a phenolic compound structurally similar to p-CA, has been previously shown to suppress ultraviolet B radiation-induced MMP expression in mouse skin [32]. Our recent study also showed that p-CA suppressed the expression of MMP1 and the phosphorylation of c-Jun N-terminal kinase in HaCaT keratinocytes exposed to ultraviolet radiation [28]. Thus, p-CA might regulate the signaling pathways that induce MMP1 expression.…”

Section: Discussionmentioning

confidence: 59%

Ascorbyl coumarates as multifunctional cosmeceutical agents that inhibit melanogenesis and enhance collagen synthesis

et al. 2015

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L-Ascorbic acid (AA) and p-coumaric acid (p-CA) are naturally occurring antioxidants that are known to enhance collagen synthesis and inhibit melanin synthesis, respectively. The purpose of this study was to examine hybrid compounds between AA and p-CA as multifunctional cosmeceutical agents. Ascorbyl 3-p-coumarate (A-3-p-C), ascorbyl 2-p-coumarate (A-2-p-C), and their parent compounds were tested for their effects on cellular melanin synthesis and collagen synthesis. At 100 μM, A-3-p-C and A-2-p-C decreased melanin content of human dermal melanocytes stimulated by L-tyrosine, by 65 and 59%, respectively, compared to 11% inhibition of AA and 70% inhibition of p-CA. A-3-p-C and A-2-p-C were less effective than p-CA but more effective than AA at inhibiting tyrosinase activity. A-3-p-C and A-2-p-C were more effective than p-CA at inhibiting the autoxidation of L-3,4-dihydroxyphenylalanine. At 100-300 μM, A-3-p-C and A-2-p-C augmented collagen release from human dermal fibroblasts by 120-144% and 125-191%, respectively, compared to 126-133% increase of AA and 120-146% increase of p-CA. They increased procollagen type I C-peptide release (A-3-p-C, and A-2-p-C) like AA, and decreased matrix metalloproteinase 1 level (A-2-p-C) like p-CA, implicating that they might regulate collagen metabolism by multiple mechanisms. This study suggests that A-3-p-C and A-2-p-C could be used as multifunctional cosmeceutical agents for the attenuation of certain aspects of skin aging.

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“…UV rays induce apoptosis of keratinocytes via intrinsic pathways, involving direct DNA damage; extrinsic pathways, involving activated cell membrane death receptors; and other ROS-mediated pathways [ 38 , 40 ]. Apoptosis can be detected using various markers, including DNA laddering, changes in the expression of pro-apoptotic (Bax, Bak, and Bid) and anti-apoptotic (Bcl-2 and Bcl-x) members of the Bcl-2 protein family, and activation of caspases [ 41 , 42 , 43 ].…”

Section: Uv-induced Toxicity In the Skinmentioning

confidence: 99%

Emerging Strategies to Protect the Skin from Ultraviolet Rays Using Plant-Derived Materials

Boo

2020

Antioxidants

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Sunlight contains a significant amount of ultraviolet (UV) ray, which leads to various effects on homeostasis in the body. Defense strategies to protect from UV rays have been extensively studied, as sunburn, photoaging, and photocarcinogenesis are caused by excessive UV exposure. The primary lines of defense against UV damage are melanin and trans-urocanic acid, which are distributed in the stratum corneum. UV rays that pass beyond these lines of defense can lead to oxidative damage. However, cells detect changes due to UV rays as early as possible and initiate cell signaling processes to prevent the occurrence of damage and repair the already occurred damage. Cosmetic and dermatology experts recommend using a sunscreen product to prevent UV-induced damage. A variety of strategies using antioxidants and anti-inflammatory agents have also been developed to complement the skin’s defenses against UV rays. Researchers have examined the use of plant-derived materials to alleviate the occurrence of skin aging, diseases, and cancer caused by UV rays. Furthermore, studies are also underway to determine how to promote melanin production to protect from UV-induced skin damage. This review provides discussion of the damage that occurs in the skin due to UV light and describes potential defense strategies using plant-derived materials. This review aims to assist researchers in understanding the current research in this area and to potentially plan future studies.

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Effects of Bambusae Caulis in Taeniam Extract on the UVB-induced Cell Death, Oxidative Stress and Matrix Metalloproteinase 1 Expression in Keratinocytes

Cited by 8 publications

References 38 publications

Scutellaria radix Extract as a Natural UV Protectant for Human Skin

Scutellaria radix Extract as a Natural UV Protectant for Human Skin

Ascorbyl coumarates as multifunctional cosmeceutical agents that inhibit melanogenesis and enhance collagen synthesis

Emerging Strategies to Protect the Skin from Ultraviolet Rays Using Plant-Derived Materials

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