Effects of BAP-NAA on plant regeneration from half cotyledon explants of Turkish Common Vetch (Vicia sativa L.) cultivar Kubilay

Mirzapour, Mohsen; Nofouzı, Farzad; Mokhtarzadeh, Sam; Kendir, Hayrettin; Khawar, Khalid Mahmood

doi:10.1016/j.copbio.2013.05.381

Cited by 3 publications

(1 citation statement)

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“…sativa) is a self-pollinated, annual and diploid leguminous forage that can adapt to different soil and climate and x atmospheric nitrogen through its symbiotic relationship with rhizobia to improve soil structure (Chooi 1971;Chung et al 2013b). As an important forage legume crop, common vetch contains high level of protein, starch, and oil and has been commonly used as green manure, pasture, silage, and hay (Chung et al 2013a;Mirzapour et al 2013). In addition to these qualities, the common vetch also has been widely used as good quality animal feedstock and health-promoting foods for human consumption (Uzun et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome-wide Development and Utilization of Novel Intron-length Polymorphic (ILP) Markers in Common Vetch (Vicia Sativa Subsp. Sativa)

Wei¹,

Wang²,

Ma³

et al. 2021

Preprint

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Common vetch (Vicia sativa subsp. sativa) is one of the most economically important forage legumes with rich nutritional value and multiple uses. Though the large-scale development and application of microsatellite markers have been conducted in common vetch germplasm evaluation, the investigation and exploitation of intron-length polymorphic (ILP) markers have not been systematically analyzed. In this study, the intron positions of common vetch genome were located by aligning the RNA-Seq sequences of common vetch with Medicago truncatula, soybean (Glycine max), and Arabidopsis thaliana genomic sequences, and used for VsILP marker development. A total of 10,400 markers were generated from 44,582 common vetch unigenes. Out of 300 randomly selected VsILP markers, 283 resulted in successful amplification in common vetch. Among these markers, 40 produced length variation in 30 common vetch accessions, collectively yielding 166 alleles with an average of 4.0 alleles per locus. The polymorphic information content (PIC) values extended from 0.06 to 0.81 with a mean of 0.49. Of the 283 VsILP markers, 84.8% exhibited transferability to leguminous and non-leguminous species. We presented here the first large-scale development of ILP markers in common vetch and their utility in germplasm evaluation and transferability, which will be valuable for further comparative genomic studies, genetic relationship assessments, and marker-assisted breeding of leguminous and non-leguminous species.

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Section: Introductionmentioning

confidence: 99%

Transcriptome-wide Development and Utilization of Novel Intron-length Polymorphic (ILP) Markers in Common Vetch (Vicia Sativa Subsp. Sativa)

Wei¹,

Wang²,

Ma³

et al. 2021

Preprint

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show abstract

Transcriptome-wide development and utilisation of novel intron-length polymorphic markers in common vetch (

Wei

Wang

et al. 2021

Crop & Pasture Science

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Common vetch (Vicia sativa subsp. sativa) is one of the most economically important forage legumes, with high nutritional value and multiple uses. Although microsatellite markers have been developed and applied on a large scale for evaluation of common vetch germplasm, intron-length polymorphic (ILP) markers have not been systematically investigated and exploited. In this study, introns within the common vetch genome were located by aligning the RNA-Seq sequences of common vetch with barrel medic (Medicago truncatula), soybean (Glycine max) and Arabidopsis thaliana genome sequences, and then used for VsILP marker development. In total, 10400 markers were generated from 44582 common vetch unigenes. Of 300 randomly selected VsILP markers, 283 were successfully amplified in common vetch. Among these markers, 40 produced length variation in 30 accessions of common vetch, collectively yielding 166 alleles with an average of 4.0 alleles per locus. The polymorphic information content values extended from 0.06 to 0.81 with a mean of 0.49. Of the 283 VsILP markers, 84.8% exhibited transferability to other species, both leguminous (common vetch, lucerne (Medicago sativa), barrel medic, soybean, yellow sweet clover (Melilotus officinalis), Lotus corniculatus and Sophora alopecuroides) and non-leguminous (rice (Oryza sativa), Arabidopsis and tobacco (Nicotiana tabacum)). Here, we present the first large-scale development of ILP markers in common vetch and their utility in germplasm evaluation and transferability, which will be valuable for further comparative genomic studies, genetic relationship assessments, and marker-assisted breeding of leguminous and non-leguminous species.

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Exploiting Illumina Sequencing for the Development of 95 Novel Polymorphic EST-SSR Markers in Common Vetch (Vicia sativa subsp. sativa)

Liu

Luo

et al. 2014

Molecules

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Abstract:The common vetch (Vicia sativa subsp. sativa), a self-pollinating and diploid species, is one of the most important annual legumes in the world due to its short growth period, high nutritional value, and multiple usages as hay, grain, silage, and green manure. The available simple sequence repeat (SSR) markers for common vetch, however, are insufficient to meet the developing demand for genetic and molecular research on this important species. Here, we aimed to develop and characterise several polymorphic EST-SSR markers from the vetch Illumina transcriptome. A total number of 1,071 potential EST-SSR markers were identified from 1025 unigenes whose lengths were greater than 1,000 bp, and 450 primer pairs were then designed and synthesized. Finally, 95 polymorphic primer pairs were developed for the 10 common vetch accessions, which included 50 individuals. Among the 95 EST-SSR markers, the number of alleles ranged from three to 13, and the polymorphism information content values ranged from 0.09 to 0.98. The observed heterozygosity values ranged from 0.00 to 1.00, and the expected heterozygosity values ranged from 0.11 to 0.98. These 95 EST-SSR markers developed from the vetch Illumina transcriptome could greatly promote the development of genetic and molecular breeding studies pertaining to in this species.

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Effects of BAP-NAA on plant regeneration from half cotyledon explants of Turkish Common Vetch (Vicia sativa L.) cultivar Kubilay

Cited by 3 publications

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Transcriptome-wide Development and Utilization of Novel Intron-length Polymorphic (ILP) Markers in Common Vetch (Vicia Sativa Subsp. Sativa)

Transcriptome-wide Development and Utilization of Novel Intron-length Polymorphic (ILP) Markers in Common Vetch (Vicia Sativa Subsp. Sativa)

Transcriptome-wide development and utilisation of novel intron-length polymorphic markers in common vetch (

Exploiting Illumina Sequencing for the Development of 95 Novel Polymorphic EST-SSR Markers in Common Vetch (Vicia sativa subsp. sativa)

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Effects of BAP-NAA on plant regeneration from half cotyledon explants of Turkish Common Vetch (Vicia sativa L.) cultivar Kubilay

Cited by 3 publications

References 0 publications

Transcriptome-wide&nbsp;Development and Utilization of Novel Intron-length&nbsp;Polymorphic (ILP) Markers in&nbsp;Common Vetch (Vicia Sativa Subsp. Sativa)

Transcriptome-wide&nbsp;Development and Utilization of Novel Intron-length&nbsp;Polymorphic (ILP) Markers in&nbsp;Common Vetch (Vicia Sativa Subsp. Sativa)

Transcriptome-wide development and utilisation of novel intron-length polymorphic markers in common vetch (

Exploiting Illumina Sequencing for the Development of 95 Novel Polymorphic EST-SSR Markers in Common Vetch (Vicia sativa subsp. sativa)

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Product

Resources

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Transcriptome-wide Development and Utilization of Novel Intron-length Polymorphic (ILP) Markers in Common Vetch (Vicia Sativa Subsp. Sativa)

Transcriptome-wide Development and Utilization of Novel Intron-length Polymorphic (ILP) Markers in Common Vetch (Vicia Sativa Subsp. Sativa)