Effects of basic fibroblast growth factor on human periodontal ligament cells

Abstract

In order to clarify the regulatory mechanisms of periodontal regeneration by basic fibroblast growth factor (bFGF), effects of bFGF on proliferation, alkaline phosphatase activity, calcified nodule formation and extracellular matrix synthesis of human periodontal ligament (PDL) cells were examined in this study. bFGF enhanced the proliferative responses of PDL cells in a dose-dependent manner. The maximum mitogenic effect of bFGF on PDL cells was observed at the concentration of 10 ng/ml. In contrast, bFGF inhibited the induction of alkaline phosphatase activity and the mineralized nodule formation by PDL cells. Moreover, employing the reverse transcription-polymerase chain reaction (RT-PCR) technique, we observed that the levels of laminin mRNA of human PDL cells was specifically upregulated by bFGF stimulation, but that of type I collagen mRNA was downregulated. On the other hand, the expression of type III collagen and fibronectin mRNA were not altered even when the cells were activated by bFGF. These results suggest that suppressing cytodifferentiation of PDL cells into mineralized tissue forming cells, bFGF may play a role in wound healing by inducing growth of immature PDL cells and that in turn accelerates periodontal regeneration.

The effect of selected growth factors on human anterior cruciate ligament cell interactions with a three‐dimensional collagen‐GAG scaffold

“…The stimulation of ACL cell proliferation by FGF-2 is consistent with previous reports [6,29,41]. The combination of cell proliferation and slowing of scaffold dimensional change may be useful for applications where it is desirable for the scaffold to maintain its shape during remodeling.…”

Periodontal regeneration following application of basic fibroblast growth factor-2 in combination with beta tricalcium phosphate in class III furcation defects in dogs

“…Murakami et al observed that downgrowth of junctional epithelium was limited by bFGF-2 application when compared with sham operation in class II furcation defects 14) . In general, bFGF-2 has been reported to induce the proliferation of not only periodontal ligament cells but also gingival epithelial cells in a dose-dependent manner in vitro 8,32) . However, Takayama et al reported that co-stimulation with fetal calf serum inhibited bFGF2-induced-proliferation of gingival epithelial cells, but synergistically enhanced bFGF-2-induced periodontal ligament cell proliferation 32) .…”

“…We attempted to determine the optimal dose of bFGF-2 for periodontal regeneration in dogs from several previous reports. bFGF-2 had a dose-dependent effect on periodontal regeneration at a dose ranging from 0 to 0.16% per implant [3][4][5][6][7][8][9][10][11][12][13][14][15][26][27][28][29] . Hayashi et al reported that new bone formation was smaller in the 0.56% bFGF-2 (100 µg bFGF-2/3×3×2 mm gelatin hydrogel) implant compared to the 0.056% bFGF-2 (10 µg bFGF-2/3×3×2 mm gelatin hydrogel) implant; however, there were no significant differences between them 29) .…”

“…Basic fibroblast growth factor-2 (bFGF-2) is a heparinbinding growth factor that exhibits potent angiogenic activity and mitogenic ability in mesenchymal cells [1][2][3][4][5][6][7] and periodontal ligament cells [8][9][10][11] . Several studies have reported that bFGF-2 induces potent periodontal tissue regeneration along with new alveolar bone and cementum formation without aberrant healing such as root resorption and ankylosis in critical size defects of animal models [12][13][14] .…”

Growth factor delivery for oral and periodontal tissue engineering

“…The angiogenic and fibroblast stimulatory properties of FGF-2 during wound healing and its chemotactic and proliferative effects on PDL cells [90,91] suggest its use for periodontal regenerative therapeutic approaches. In preclinical studies, this growth factor was evaluated for the treatment of different types of periodontal bone defects, in dogs [92][93][94][95] and non-human primates [96].…”