Effects of Bifidobacterium longum Subsp. infantis CECT 7210 and Lactobacillus rhamnosus HN001, Combined or Not With Oligofructose-Enriched Inulin, on Weaned Pigs Orally Challenged With Salmonella Typhimurium

Rodríguez-Sorrento, Agustina; Castillejos, Lorena; López-Colom, Paola; Cifuentes-Orjuela, Gloria; Rodrı́guez-Palmero, Marı́a; Muñoz, José Antonio Moreno; Martín‐Orúe, S. M.

doi:10.3389/fmicb.2020.02012

Cited by 14 publications

(19 citation statements)

References 46 publications

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“…Similarly, L. rhamnosus HN001 is successful against pathogens such as ETEC and Staphylococcus aureus (Gopal et al, 2001;Inturri et al, 2016;Eggers et al, 2018). More recently, in previous studies of our group, we could also demonstrate the positive effects of this probiotic combination against a Salmonella challenge, leading to a faster clearance of the pathogen and recovery from the intestinal damage (Rodríguez-Sorrento et al, 2020). This putative reduction in the enteropathogen challenge promoted by the probiotic treatment could have also explained the intermediate level of mitosis observed between the CTR+ and CTR− groups at day 4 PI and the faster recovery of villus height at day 8 PI.…”

Section: Discussionsupporting

confidence: 57%

“…By contrast, Krumbeck et al (2018) did not find any synergy in the capacity of improvement intestinal barrier function when combining bifidobacteria and GOS in humans. Previous studies of our group also failed to demonstrate the synergic activities of combination of the strains CECT 7210 and HN001 with FOS in Samonella orally challenged piglets (Rodríguez-Sorrento et al, 2020). In the present study, we observed some synergy before the challenge, particularly in the number of enterobacteria and coliforms, which were lower compared to the rest of supplemented groups.…”

Section: Discussionsupporting

confidence: 50%

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Effects of the Administration of Bifidobacterium longum subsp. infantis CECT 7210 and Lactobacillus rhamnosus HN001 and Their Synbiotic Combination With Galacto-Oligosaccharides Against Enterotoxigenic Escherichia coli F4 in an Early Weaned Piglet Model

et al. 2021

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We evaluated the potential of multi-strain probiotic (Bifidobacterium longum subsp. infantis CECT 7210 and Lactobacillus rhamnosus HN001) with or without galacto-oligosaccharides against enterotoxigenic Escherichia coli (ETEC) F4 infection in post-weaning pigs. Ninety-six piglets were distributed into 32 pens assigned to five treatments: one non-challenged (CTR+) and four challenged: control diet (CTR−), with probiotics (>3 × 1010 CFU/kg body weight each, PRO), prebiotic (5%, PRE), or their combination (SYN). After 1 week, animals were orally inoculated with ETEC F4. Feed intake, weight, and clinical signs were recorded. On days 4 and 8 post-inoculation (PI), one animal per pen was euthanized and samples from blood, digesta, and tissues collected. Microbiological counts, ETEC F4 real-time PCR (qPCR) quantification, fermentation products, serum biomarkers, ileal histomorphometry, and genotype for mucin 4 (MUC4) polymorphism were determined. Animals in the PRO group had similar enterobacteria and coliform numbers to the CTR+ group, and the ETEC F4 prevalence, the number of mitotic cells at day 4 PI, and villus height at day 8 PI were between that observed in the CTR+ and CTR− groups. The PRO group exhibited reduced pig major acute-phase protein (Pig-MAP) levels on day 4 PI. The PRE diet group presented similar reductions in ETEC F4 and Pig-MAP, but there was no effect on microbial groups. The SYN group showed reduced fecal enterobacteria and coliform counts after the adaptation week but, after the inoculation, the SYN group showed lower performance and more animals with high ETEC F4 counts at day 8 PI. SYN treatment modified the colonic fermentation differently depending on the MUC4 polymorphism. These results confirm the potential of the probiotic strains and the prebiotic to fight ETEC F4, but do not show any synergy when administered together, at least in this animal model.

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Section: Discussionsupporting

confidence: 57%

Section: Discussionsupporting

confidence: 50%

Effects of the Administration of Bifidobacterium longum subsp. infantis CECT 7210 and Lactobacillus rhamnosus HN001 and Their Synbiotic Combination With Galacto-Oligosaccharides Against Enterotoxigenic Escherichia coli F4 in an Early Weaned Piglet Model

et al. 2021

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“…2g ), and a decrease in labile polysaccharide and polypeptide concentrations. The occurrence of such a high bacterial abundance is not rare inside natural sinking particles 26 , 39 , 40 . The growth rates observed in the model are high for the temperature regime but conceivable given that there are some reports of extremely high growth rates in particle-associated bacteria albeit at higher temperatures and in different environments than modeled here 41 , 42 .…”

Section: Resultsmentioning

confidence: 99%

Quantifying nitrogen fixation by heterotrophic bacteria in sinking marine particles

et al. 2021

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Nitrogen ($${{\rm{N}}}_{2}$$ N 2 ) fixation by heterotrophic bacteria associated with sinking particles contributes to marine N cycling, but a mechanistic understanding of its regulation and significance are not available. Here we develop a mathematical model for unicellular heterotrophic bacteria growing on sinking marine particles. These bacteria can fix $${{\rm{N}}}_{2}$$ N 2 under suitable environmental conditions. We find that the interactive effects of polysaccharide and polypeptide concentrations, sinking speed of particles, and surrounding $${{\rm{O}}}_{2}$$ O 2 and $${{{\rm{NO}}}_{3}}^{-}$$ NO 3 − concentrations determine the $${{\rm{N}}}_{2}$$ N 2 fixation rate inside particles. $${{\rm{N}}}_{2}$$ N 2 fixation inside sinking particles is mainly fueled by $${{{\rm{SO}}}_{4}}^{2-}$$ SO 4 2 − respiration rather than $${{{\rm{NO}}}_{3}}^{-}$$ NO 3 − respiration. Our model suggests that anaerobic processes, including heterotrophic $${{\rm{N}}}_{2}$$ N 2 fixation, can take place in anoxic microenvironments inside sinking particles even in fully oxygenated marine waters. The modelled $${{\rm{N}}}_{2}$$ N 2 fixation rates are similar to bulk rates measured in the aphotic ocean, and our study consequently suggests that particle-associated heterotrophic $${{\rm{N}}}_{2}$$ N 2 fixation contributes significantly to oceanic $${{\rm{N}}}_{2}$$ N 2 fixation.

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“…A combinational use of multiple alternatives providing potential synergic benefits, so called synbiotics, is leading a recent trend of pig nutrition research [25,[81][82][83].…”

Section: Nutritional Strategies Targeting Intestinal Healthmentioning

confidence: 99%

Understanding intestinal health in nursery pigs and the relevant nutritional strategies

Kim

Duarte

2021

Anim Biosci

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In the modern pig production, pigs are weaned at early age with immature intestine. Dietary and environmental factors challenge the intestine, specifically the jejunum, causing inflammation and oxidative stress followed by destruction of epithelial barrier and villus structures in the jejunum. Crypt cell proliferation increases to repair damages in the jejunum. Challenges to maintain the intestinal health have been shown to be related to changes in the profile of mucosa-associated microbiota in the jejunum of nursery pigs. All these processes can be quantified as biomarkers to determine status of intestinal health related to growth potential of nursery pigs. Nursery pigs with impaired intestinal health show reduced ability of nutrient digestion and thus reduced growth. A tremendous amount of research effort has been made to determine nutritional strategies to maintain or improve intestinal health and microbiota in nursery pigs. A large number of feed additives have been evaluated for their effectiveness on improving intestinal health and balancing intestinal microbiota in nursery pigs. Selected prebiotics, probiotics, postbiotics, and other bioactive compounds can be used in feeds to handle issues with intestinal health. Selection of these feed additives should aim modulating biomarkers indicating intestinal health. This review aims to define intestinal health and introduce examples of nutritional approaches to handle intestinal health in nursery pigs.

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Effects of Bifidobacterium longum Subsp. infantis CECT 7210 and Lactobacillus rhamnosus HN001, Combined or Not With Oligofructose-Enriched Inulin, on Weaned Pigs Orally Challenged With Salmonella Typhimurium

Cited by 14 publications

References 46 publications

Effects of the Administration of Bifidobacterium longum subsp. infantis CECT 7210 and Lactobacillus rhamnosus HN001 and Their Synbiotic Combination With Galacto-Oligosaccharides Against Enterotoxigenic Escherichia coli F4 in an Early Weaned Piglet Model

Effects of the Administration of Bifidobacterium longum subsp. infantis CECT 7210 and Lactobacillus rhamnosus HN001 and Their Synbiotic Combination With Galacto-Oligosaccharides Against Enterotoxigenic Escherichia coli F4 in an Early Weaned Piglet Model

Quantifying nitrogen fixation by heterotrophic bacteria in sinking marine particles

Understanding intestinal health in nursery pigs and the relevant nutritional strategies

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