Effects of BMP-2 Delivery in Calcium Phosphate Bone Graft Materials with Different Compositions on Bone Regeneration

Park, Jin Chul; Bae, Eun‐Bin; Kim, Se Eun; Kim, So Yun; Choi, Kyung Hee; Choi, Jae Won; Bae, Ji Hyeon; Ryu, Jae Jun; Huh, Jung-Bo

doi:10.3390/ma9110954

Cited by 10 publications

(5 citation statements)

References 40 publications

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“…According to study of Hänseler et al (2015) the DBB is capable of retaining half of the rhBMP‐2 adsorbed in differentiation medium during 15 days. In contrast, more than 90% rhBMP‐2 adsorbed to HA/TCP (BCP) is release during the initial 24 hr, similar to those reported by Park et al (2016). In pBCP, the high initial burst release of F1 increased vascular permeability, causing vessel leakage, and inducing the formation of malformed and nonfunctional blood vessels, as observed in high concentration of VEGF (Drake & Little, 1995; Horowitz et al, 1997; Vajanto et al, 2002).…”

Section: Discussionsupporting

confidence: 86%

Concentration‐dependent effects of latex F1‐protein fraction incorporated into deproteinized bovine bone and biphasic calcium phosphate on the repair of critical‐size bone defects

et al. 2020

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F1-protein fraction (F1) is a natural bioactive compound extracted from the rubber tree, Hevea brasiliensis, and has been recently studied for its therapeutic potential in wound healing. In this study, we investigated the concentration-dependent effects of F1 (0.01%, 0.025%, 0.05%, and 0.1%) incorporated into deproteinized bovine bone (DBB) and porous biphasic calcium phosphate (pBCP), on the repair of rat calvarial critical-size bone defects (CSBD). The defects were analyzed by 3Dmicrotomography and 2D-histomorphometry at 12 weeks postsurgery. The binding efficiency of F1 to pBCP (96.3 ± 1.4%) was higher than that to DBB (67.7 ± 3.3%). In vivo analysis showed a higher bone volume (BV) gain in all defects treated with DBB (except in 0.1% of F1) and pBCP (except in 0.05% and 0.1% of F1) compared to the CSBD without treatment/control group (9.96 ± 2.8 mm 3). DBB plus 0.025% F1 promoted the highest BV gain (29.7 ± 2.2 mm 3 , p < .0001) compared to DBB without F1 and DBB plus 0.01% and 0.1% of F1. In the pBCP group, incorporation of F1 did not promote bone gain when compared to pBCP without F1 (15.9 ± 4.2 mm 3 , p > .05). Additionally, a small BV occurred in defects treated with pBCP plus 0.1% F1 (10.4 ± 1.4 mm 3, p < .05). In conclusion, F1 showed a higher bone formation potential in combination with DBB than with pBCP, in a concentration-dependent manner. Incorporation of 0.25% F1 into DBB showed the best results with respect to bone formation/repair in CSBD. These results suggest that DBB plus 0.25% F1 can be used as a promising bioactive material for application in bone tissue engineering. K E Y W O R D S bone repair, deproteinized bovine bone, natural latex protein fraction, porous biphasic calcium phosphate, protein adsorption, rat calvarial critical-size bone defects 1 | INTRODUCTION Bone defects can heal spontaneously under suitable physiological and environmental conditions due to the bone's ability to repair (Cameron et al., 2013). However, large skeletal defects resulting from malformation, infection, trauma, or tumor resection are challenging problems in contemporary reconstructive surgery because the size of these defects exceed the bone's natural capacity to repair (Henkel et al.,

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Section: Discussionsupporting

confidence: 86%

Concentration‐dependent effects of latex F1‐protein fraction incorporated into deproteinized bovine bone and biphasic calcium phosphate on the repair of critical‐size bone defects

et al. 2020

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“…In view of the aforesaid characteristics, encouraging results emerged when BCPs were used alone [14,15] or as carriers [16,17] to promote and enhance bone regeneration in oral surgery. On the other hand, the porosity [18,19] and configurations [20,21] of BCPs are still source of investigations in view of their influence on the bioactive and bioabsorption properties.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Osteoconduction of Biphasic Calcium Phosphate Ceramic in the Calvaria of Rats: Microscopic and Histometric Analysis

Puttini

Poli

Maiorana

et al. 2019

JFB

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(1) Background: Evaluate the osteoconduction capability of a biphasic calcium phosphate (BCP) ceramic composed of hydroxyapatite and β-tricalcium phosphate 60%/40% in a rat model. (2) Methods: In the calvarial bone of 54 adult male rats, 7-mm diameter critical size defects were performed. The animals were randomly allocated to three experimental groups according to the type of material: blood clot (BCG), blood clot covered with a bovine-derived collagen membrane (MBCG), and BCP ceramic covered with a bovine-derived collagen membrane (BCPG). In each group, 6 animals were euthanatized at post-operative days 7, 30, and 60 for histological and histometric analysis. (3) Results: The qualitative analysis revealed the persistence of the collagen membrane at seven days, with no relevant newly bone formation in all groups. At 30 days, centripetal bone formation was observed residual particles of the biomaterial surrounded by fibroblasts noted in the BCPG. At 60 days, while BCG and MBCG showed a partial maturation with the central part of the defect populated by a fibrous connective tissue, in the BCPG the critical area was entirely occupied by newly formed bone. In the intra groups analysis was noted a significant increase in new bone formation during the experimental period (p < 0.05). At 60 days, BCPG showed a higher percentage area of new bone formation (p < 0.05). (4) Conclusion: BCP promoted a new bone formation by osteoconduction and might be considered a valid alternative in bone regeneration procedures.

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“…In addition, rhBMP-2 showed the potential of added autoinducing in this study and proved its potential, but no resolution of carcinogenesis, dosage, long-term results, and suitable carriers has been reported. We did not investigate the kinetic release of the rhBMP-2 in this study and instead referred to the dose concentration used in the previous article [69]. It would be meaningful to measure the releasing profile of rhBMP-2 in the Boneplant/CMC/BMP group, but this study excluded this test because the difference between fast release and slow release of rhBMP-2 was not the main evaluation variable.…”

Section: Discussionmentioning

confidence: 99%

Bone Regeneration Using a Three-Dimensional Hexahedron Channeled BCP Block Combined with Bone Morphogenic Protein-2 in Rat Calvarial Defects

et al. 2019

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It is important to obtain sufficient bone mass before implant placement on alveolar bone, and synthetic bone such as biphasic calcium phosphate (BCP) has been studied to secure this. This study used a BCP block bone with a specific structure of the three-dimensional (3D) hexahedron channel and coating with recombinant human bone morphogenetic protein-2 (rhBMP-2) impregnated carboxymethyl cellulose (CMC) was used to examine the enhancement of bone regeneration of this biomaterial in rat calvarial defect. After the preparation of critical-size calvarial defects in fifteen rats, defects were divided into three groups and were implanted with the assigned specimen (n = 5): Boneplant (untreated 3D hexahedron channeled BCP block), Boneplant/CMC (3D hexahedron channeled BCP block coated with CMC), and Boneplant/CMC/BMP (3D hexahedron channeled BCP block coated with CMC containing rhBMP-2). After 4 weeks, the volumetric, histologic, and histometric analyses were conducted to measure the newly formed bone. Histologically, defects in the Boneplant/CMC/BMP group were almost completely filled with new bone compared to the Boneplant and Boneplant/CMC groups. The new bone volume (P < 0.05) and area (P < 0.001) in the Boneplant/CMC/BMP group (20.12% ± 2.17, 33.79% ± 3.66) were much greater than those in the Boneplant (10.77% ± 4.8, 16.48% ± 9.11) and Boneplant/CMC (10.72% ± 3.29, 16.57% ± 8.94) groups, respectively. In conclusion, the 3D hexahedron channeled BCP block adapted rhBMP-2 with carrier CMC showed high possibility as an effective bone graft material.

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Effects of BMP-2 Delivery in Calcium Phosphate Bone Graft Materials with Different Compositions on Bone Regeneration

Cited by 10 publications

References 40 publications

Concentration‐dependent effects of latex F1‐protein fraction incorporated into deproteinized bovine bone and biphasic calcium phosphate on the repair of critical‐size bone defects

Concentration‐dependent effects of latex F1‐protein fraction incorporated into deproteinized bovine bone and biphasic calcium phosphate on the repair of critical‐size bone defects

Evaluation of Osteoconduction of Biphasic Calcium Phosphate Ceramic in the Calvaria of Rats: Microscopic and Histometric Analysis

Bone Regeneration Using a Three-Dimensional Hexahedron Channeled BCP Block Combined with Bone Morphogenic Protein-2 in Rat Calvarial Defects

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